Methylene blue (purity≥70%)
Based on 1 Customer Validation
Methylene blue (purity≥70%) is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue (purity≥70%) is a vasopressor and is often used as a dye in several medical procedures. Methylene blue (purity≥70%) through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue (purity≥70%) is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue (purity≥70%) is a Tau aggregation inhibitor. Methylene blue reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation.
For research use only. We do not sell to patients.
- Purity: 95.0%
- CAS No.: 61-73-4
- Formula: C16H18ClN3S
- Molecular Weight:319.86
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| B16-F10 | IC50 |
5.48 μM
Compound: MB
|
Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in light irradiation and measured for 48 hrs by MTT assay
Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in light irradiation and measured for 48 hrs by MTT assay
|
[PMID: 34871840] |
| B16-F10 | IC50 |
7.02 μM
Compound: MB
|
Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
|
[PMID: 34871840] |
| Fibroblast | EC50 |
43 nM
Compound: MB
|
Inhibition of erastin-induced cell death in FRDA patient-derived fibroblast assessed as depletion of cellular ATP incubated for 12 hrs followed by erastin stimulation and measured after by luciferase-linked ATPase enzymatic assay
Inhibition of erastin-induced cell death in FRDA patient-derived fibroblast assessed as depletion of cellular ATP incubated for 12 hrs followed by erastin stimulation and measured after by luciferase-linked ATPase enzymatic assay
|
[PMID: 33214825] |
| HepG2 | IC50 |
<1 μM
Compound: Methylene blue
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Antimalarial activity against sporozoite stage of Plasmodium yoelii assessed as invasion of human HepG2 cells expressing CD81 incubated for 2 hrs prior to inoculation measured after 1 hr by immunofluorescence assay in presence of penicillin/streptomycin
Antimalarial activity against sporozoite stage of Plasmodium yoelii assessed as invasion of human HepG2 cells expressing CD81 incubated for 2 hrs prior to inoculation measured after 1 hr by immunofluorescence assay in presence of penicillin/streptomycin
|
[PMID: 23927658] |
| HepG2 | IC50 |
6 μM
Compound: MB
|
Anticancer activity against human HepG2 cells
Anticancer activity against human HepG2 cells
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[PMID: 33460833] |
| HT-29 | IC50 |
>10 μM
Compound: MB
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Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 24 hrs in dark by MTT assay
Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 24 hrs in dark by MTT assay
|
[PMID: 32267685] |
| HT-29 | IC50 |
9.2 μM
Compound: MB
|
Cytotoxicity against human HT-29 cells assessed as reduction in cell viability preincubated for 1 hr followed by light irradiation at 3.2 J/cm2 for 30 sec and measured after 24 hrs by MTT assay
Cytotoxicity against human HT-29 cells assessed as reduction in cell viability preincubated for 1 hr followed by light irradiation at 3.2 J/cm2 for 30 sec and measured after 24 hrs by MTT assay
|
[PMID: 32267685] |
| Lymphocyte | EC50 |
88 nM
Compound: MB
|
Anti-ferroptotic activity in FRDA patient-derived Lymphocyte assessed as reduction in RSL3-induced lipid peroxidation incubated for overnight followed by RSL3 stimulation and measured after 90 mins by FACS analysis
Anti-ferroptotic activity in FRDA patient-derived Lymphocyte assessed as reduction in RSL3-induced lipid peroxidation incubated for overnight followed by RSL3 stimulation and measured after 90 mins by FACS analysis
|
[PMID: 33214825] |
| MCF7 | IC50 |
10.58 μM
Compound: MB
|
Antiproliferative activity against human MCF7 cells assessed as cell viability in light irradiation and measured after 48 hrs by MTT assay
Antiproliferative activity against human MCF7 cells assessed as cell viability in light irradiation and measured after 48 hrs by MTT assay
|
[PMID: 34871840] |
| MCF7 | IC50 |
14.64 μM
Compound: MB
|
Antiproliferative activity against human MCF7 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
Antiproliferative activity against human MCF7 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
|
[PMID: 34871840] |
Guidelines (The following is our recommended protocol. This protocol is provided for reference only and should be modified according to your specific needs).
1. Sample treatment
1.1 For paraffin sections: dewax and rehydrate as usual.
1.2 For frozen sections: distilled water for 2 min.
1.3 For cultured cells: Fix with 4% paraformaldehyde. Wash with distilled water for 2 min. Replace with fresh distilled water and wash for another 2 min.
2. Methylene blue staining
2.1 Stain with 0.1% Methylene blue for 2 min.
2.2 Wash thoroughly with distilled water, observe and take photos.
2.3 Staining results:
Tissue or cells: blue
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 61-73-4
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Appearance Solid
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Molecular Weight 319.86
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Formula C16H18ClN3S
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Color Brown to black
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SMILES
CN(C)C1=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C2C=C1.[Cl-]
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Synonyms
Basic Blue 9 (purity≥70%); CI-52015 (purity≥70%); Methylthioninium chloride (purity≥70%)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 20 mg/mL (62.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (397 KB)
- English - EN (397 KB)
- Français - FR (397 KB)
- Deutsch - DE (397 KB)
- Norwegian - NO (397 KB)
- Español - ES (397 KB)
- Swedish - SV (397 KB)
- Italian - IT (397 KB)
- Portuguese - PT (397 KB)
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Handling Instructions (2659 KB)
References
[1]. Klamer D, et, al. Phencyclidine-induced behaviour in mice prevented by methylene blue. Basic Clin Pharmacol Toxicol. 2004 Feb;94(2):65-72. [Content Brief]
[2]. Hochgräfe K, et, al. Preventive methylene blue treatment preserves cognition in mice expressing full-length pro-aggregant human Tau. Acta Neuropathol Commun. 2015 May 10;3:25. [Content Brief]
[3]. Fenn AM, et, al. Methylene blue attenuates traumatic brain injury-associated neuroinflammation and acute depressive-like behavior in mice. J Neurotrauma. 2015 Jan 15;32(2):127-38. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1264 mL | 15.6318 mL | 31.2637 mL | 78.1592 mL |
| 5 mM | 0.6253 mL | 3.1264 mL | 6.2527 mL | 15.6318 mL | |
| 10 mM | 0.3126 mL | 1.5632 mL | 3.1264 mL | 7.8159 mL | |
| 15 mM | 0.2084 mL | 1.0421 mL | 2.0842 mL | 5.2106 mL | |
| 20 mM | 0.1563 mL | 0.7816 mL | 1.5632 mL | 3.9080 mL | |
| 25 mM | 0.1251 mL | 0.6253 mL | 1.2505 mL | 3.1264 mL | |
| 30 mM | 0.1042 mL | 0.5211 mL | 1.0421 mL | 2.6053 mL | |
| 40 mM | 0.0782 mL | 0.3908 mL | 0.7816 mL | 1.9540 mL | |
| 50 mM | 0.0625 mL | 0.3126 mL | 0.6253 mL | 1.5632 mL | |
| 60 mM | 0.0521 mL | 0.2605 mL | 0.5211 mL | 1.3027 mL |
Methylene blue (purity≥70%) Related Classifications
Cat. No.: HY-14536A
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- Methylene blue (purity≥70%)
- 61-73-4
- Basic Blue 9 (purity≥70%)
- CI-52015 (purity≥70%)
- Methylthioninium chloride (purity≥70%)
- Environmental Pollutants
- Microtubule/Tubulin
- Guanylate Cyclase
- Monoamine Oxidase
- NO Synthase
- vasopressor
- dye
- antinociception
- antimalarial
- antidepressant
- anxiolytic
- methemoglobinemias
- cGMP
- Inhibitor
- inhibitor
- inhibit