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  5. Methylnitronitrosoguanidine

Methylnitronitrosoguanidine  (Synonyms: MNNG)

製品番号: HY-128612 純度: 99.97%
COA 取扱説明書 Technical Support

Methylnitronitrosoguanidine (MNNG) is an orally active alkylating agent with toxic and mutagenic effects. Methylnitronitrosoguanidine can as a carcinogen and mutagen. 50% water content, specifications are based on dry weight.

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CAS 番号 : 70-25-7

容量 価格（税別） 在庫状況 数量
1 g $180 在庫あり
5 g $450 在庫あり
10 g   お問い合わせ  
50 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 15 publication(s) in Google Scholar

Methylnitronitrosoguanidine 関連抗体

Top Publications Citing Use of Products

    Methylnitronitrosoguanidine purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2025 Apr 9:345:119591.  [Abstract]

    N-methyl-N'-nitro-N-nitrosoguanidine (MNNG, 60 μM, 24 h) increased TNF-α, IL-1β, and IL-12 and decreased IL-10 in GES-1 cells.

    Methylnitronitrosoguanidine purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2025 Apr 9:345:119591.  [Abstract]

    N-methyl-N'-nitro-N-nitrosoguanidine (MNNG, 60 μM, 24 h) induced apoptosis in GES-1 cells.

    Methylnitronitrosoguanidine purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2025 Apr 9:345:119591.  [Abstract]

    N-methyl-N'-nitro-N-nitrosoguanidine (MNNG, 60 μM, 24 h) upregulated the mRNA expression of PTEN, PI3K, AKT, Bcl-2, and Caspase-3, while downregulating Bax mRNA in GES-1 cells.

    Methylnitronitrosoguanidine purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2025 Apr 9:345:119591.  [Abstract]

    N-methyl-N'-nitro-N-nitrosoguanidine (MNNG, 60 μM, 24 h) increased the expression of PTEN, PI3K, AKT, Bcl-2, and Caspase-3, and decreased the expression of Bax in GES-1 cells.

    Methylnitronitrosoguanidine purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Oct 27;15(10):779.  [Abstract]

    N-methyl-N'-nitro-N-nitrosoguanidine (MNNG, 0.5 mM, 16 h) induced cell death in WT and ZBP1−/− dermal fibroblasts.

    Methylnitronitrosoguanidine purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Oct 27;15(10):779.  [Abstract]

    N-methyl-N'-nitro-N-nitrosoguanidine (MNNG, 0.5 mM, 16 h) induced Casp-3 cleavage, MLKL phosphorylation, and GSDME cleavage in WT dermal fibroblasts.

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    製品説明

    Methylnitronitrosoguanidine (MNNG) is an orally active alkylating agent with toxic and mutagenic effects. Methylnitronitrosoguanidine can as a carcinogen and mutagen[1][2]. 50% water content, specifications are based on dry weight.

    体外実験

    Methylnitronitrosoguanidine (MNNG, 0-1100 nM; 24 h) has toxic and mutagenic effects among 3 human diploid lymphoblast lines, MIT-2, WI-L2, and GM 130[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Methylnitronitrosoguanidine 関連抗体
    体内実験

    Note:
    Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

    Methylnitronitrosoguanidine (MNNG) is an alkylating agent that can be used to induce models of chronic atrophic gastritis and gastric cancer. In complete medium, the half-life of MNNG is approximately 70 minutes[3].

    Induction of chronic atrophic gastritis[4]
    Background
    MNNG mainly simulates the transformation of nitrate into nitrosamine and other carcinogens in the stomach, which leads to chronic atrophic gastritis, dysplasia and even gastric carcinoma.
    Specific Modeling Methods
    Rat: Sprague-Dawley (SD) • male • 200 ~ 220 g (period: 10 weeks)
    Administration: fed with pure water containing 170 μg/ml MNNG for 10 weeks
    Note
    (1) Pure water containing 170 μg/ml MNNG is stored in black bottles and refreshed every day since MNNG is not stable in solvent.
    Modeling Indicators
    Histopathological changes : Gastric mucosa pathological changes detected by HE staining.
    Opposite Product(s): HY-N0103

    Induction of gastric carcinoma[5]
    Background
    MNNG is an alkylating agent, and its carcinogenicity is mainly due to its ability to covalently bond with the bases in DNA molecules to form alkylation products. This alkylation alters the way DNA is normally constructed and replicated, which leads to mutations in cells that can then develop into cancer.
    Specific Modeling Methods
    Rat: Wistar • male • 17w (period: 2 weeks)
    Administration: 200 mg/kg • po • once daily for 2 weeks
    Note
    (1) Rats were given MNNG for 2 weeks followed by oral administration of NaCl (1 mL/rat) once every 3 days for 4 weeks.
    (2) After 33 weeks, animals were allowed to fast and then were killed.
    Modeling Indicators
    Histopathological changes : Gastric mucosa pathological changes detected by HE staining.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Wistar albino rats[2]
    Dosage: 200 mg/kg
    Administration: Oral administration, at 0 and 14th days
    Result: Had the incidence of gastric cancer in Wistar albino rats is 100%.
    分子量

    147.09

    分子式

    C2H5N5O3
    CAS 番号
    Appearance

    Solid-Liquid Mixture

    Color

    Light yellow to yellow

    SMILES

    N=C(N[N+]([O-])=O)N(C)N=O
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Pure form -20°C 3 years

    *The compound is unstable in solutions, freshly prepared is recommended.
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (679.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 6.7986 mL 33.9928 mL 67.9856 mL
    5 mM 1.3597 mL 6.7986 mL 13.5971 mL
    10 mM 0.6799 mL 3.3993 mL 6.7986 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (14.14 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (14.14 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *The compound is unstable in solutions, freshly prepared is recommended.

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.97%

    COA (245 KB) HNMR (232 KB) RP-HPLC (244 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 6.7986 mL 33.9928 mL 67.9856 mL 169.9640 mL
    5 mM 1.3597 mL 6.7986 mL 13.5971 mL 33.9928 mL
    10 mM 0.6799 mL 3.3993 mL 6.7986 mL 16.9964 mL
    15 mM 0.4532 mL 2.2662 mL 4.5324 mL 11.3309 mL
    20 mM 0.3399 mL 1.6996 mL 3.3993 mL 8.4982 mL
    25 mM 0.2719 mL 1.3597 mL 2.7194 mL 6.7986 mL
    30 mM 0.2266 mL 1.1331 mL 2.2662 mL 5.6655 mL
    40 mM 0.1700 mL 0.8498 mL 1.6996 mL 4.2491 mL
    50 mM 0.1360 mL 0.6799 mL 1.3597 mL 3.3993 mL
    60 mM 0.1133 mL 0.5665 mL 1.1331 mL 2.8327 mL
    80 mM 0.0850 mL 0.4249 mL 0.8498 mL 2.1245 mL
    100 mM 0.0680 mL 0.3399 mL 0.6799 mL 1.6996 mL
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    Methylnitronitrosoguanidine Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Methylnitronitrosoguanidine70-25-7MNNGDNA Alkylator/CrosslinkertoxicmutageniccarcinogenmutagenInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
    HY-128612
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