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  4. N-Carbamoyl-L-aspartic acid

N-Carbamoyl-L-aspartic acid  (Synonyms: L-Ureidosuccinic acid)

製品番号: HY-128425A 純度: 99.83%
COA 取扱説明書 Technical Support

N-Carbamoyl-L-aspartic acid (L-Ureidosuccinic acid) is an important pyrimidine metabolic precursor and intermediate metabolite. N-Carbamoyl-L-aspartic acid reverses the growth inhibition of Ura+ strains induced by 2-thiouracil (with growth rate increasing linearly with its concentration), but fails to support the growth of uracil-requiring Ura- strains. N-Carbamoyl-L-aspartic acid inhibits the cell growth of *Saccharomyces cerevisiae* by suppressing the purine biosynthetic pathway at a pre-step of 5-aminoimidazole nucleotide synthesis. The growth inhibitory effect of N-Carbamoyl-L-aspartic acid on yeast can be alleviated by purines, and the sensitivity of strains is closely related to the activity level of dihydroorotase.

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N-Carbamoyl-L-aspartic acid

N-Carbamoyl-L-aspartic acid 構造式

CAS 番号 : 13184-27-5

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 82 在庫あり
Solution
10 mM * 1 mL in Water USD 82 在庫あり
Solid
5 mg $75 在庫あり
10 mg $120 在庫あり
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Other Forms of N-Carbamoyl-L-aspartic acid:

N-Carbamoyl-L-aspartic acid 関連抗体

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製品説明

N-Carbamoyl-L-aspartic acid (L-Ureidosuccinic acid) is an important pyrimidine metabolic precursor and intermediate metabolite. N-Carbamoyl-L-aspartic acid reverses the growth inhibition of Ura+ strains induced by 2-thiouracil (with growth rate increasing linearly with its concentration), but fails to support the growth of uracil-requiring Ura- strains. N-Carbamoyl-L-aspartic acid inhibits the cell growth of *Saccharomyces cerevisiae* by suppressing the purine biosynthetic pathway at a pre-step of 5-aminoimidazole nucleotide synthesis. The growth inhibitory effect of N-Carbamoyl-L-aspartic acid on yeast can be alleviated by purines, and the sensitivity of strains is closely related to the activity level of dihydroorotase[1][2].

体外実験

N-Carbamoyl-L-aspartic acid (25-100 μg/mL, 10 mg/mL; up to 70 hrs) potently inhibits growth of Saccharomyces cerevisiae wild type FL 100, ura2-60, ura4-20, and ure1 strains, while ura1-8, ura3-6, and ura5-5 strains are highly resistant to this inhibition[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

N-Carbamoyl-L-aspartic acid 関連抗体

Cell Proliferation Assay[1]

Cell Line: Saccharomyces cerevisiae strains (wild type FL 100, ura2-60, ura4-20, ure1, ura1-8, ura3-6, ura5-5)
Concentration: 25 μg/mL, 50 μg/mL, 100 μg/mL, 10 mg/mL; 100 μg/mL
Incubation Time: up to 70 hrs; 375 min
Result: Dramatically inhibited growth of wild type FL 100, ura2-60, ura4-20, and ure1 strains, with initial reduced growth rates that returned to normal after 12-20 hrs (depending on concentration).
Matched untreated cell density by 48 hrs with 100 μg/mL treatment.
Showed no concentration-dependent recovery of normal growth rate in ura4-20 strain.
Only slightly or not inhibited ura1-8, ura3-6, and ura5-5 strains.
Created a halo of no growth surrounding ura4-20 streak near ura2-60 lawns, confirming it is the inhibitory enantiomorph.
Showed unimpeded growth of USA-resistant ura2-60 derivative adjacent to ura4-20.
分子量

176.13

分子式

C5H8N2O5
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

O=C(O)C[C@@H](C(O)=O)NC(N)=O
Structure Classification
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

H2O : 31.25 mg/mL (177.43 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.6776 mL 28.3881 mL 56.7762 mL
5 mM 1.1355 mL 5.6776 mL 11.3552 mL
10 mM 0.5678 mL 2.8388 mL 5.6776 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
純度とドキュメンテーション

純度: 99.83%

COA (247 KB) HNMR (241 KB) LCMS (322 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 5.6776 mL 28.3881 mL 56.7762 mL 141.9406 mL
5 mM 1.1355 mL 5.6776 mL 11.3552 mL 28.3881 mL
10 mM 0.5678 mL 2.8388 mL 5.6776 mL 14.1941 mL
15 mM 0.3785 mL 1.8925 mL 3.7851 mL 9.4627 mL
20 mM 0.2839 mL 1.4194 mL 2.8388 mL 7.0970 mL
25 mM 0.2271 mL 1.1355 mL 2.2710 mL 5.6776 mL
30 mM 0.1893 mL 0.9463 mL 1.8925 mL 4.7314 mL
40 mM 0.1419 mL 0.7097 mL 1.4194 mL 3.5485 mL
50 mM 0.1136 mL 0.5678 mL 1.1355 mL 2.8388 mL
60 mM 0.0946 mL 0.4731 mL 0.9463 mL 2.3657 mL
80 mM 0.0710 mL 0.3549 mL 0.7097 mL 1.7743 mL
100 mM 0.0568 mL 0.2839 mL 0.5678 mL 1.4194 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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N-Carbamoyl-L-aspartic acid Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-Carbamoyl-L-aspartic acid13184-27-5L-Ureidosuccinic acidFungalcytosineade2 mutantpyrimidine metabolismSaccharomyces cerevisiaeEscherichia colipurine biosynthetic pathwayadeninedihydroorotase5-aminoimidazole ribonucleotideuracilInhibitorinhibitorinhibit

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製品名:
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