N-Carbamoyl-DL-aspartic acid
Based on 1 Customer Validation
N-Carbamoyl-DL-aspartic acid (Ureidosuccinic acid) is a precursor of nucleic acid pyrimidines. N-Carbamoyl-DL-aspartic acid has antitumor activities.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 923-37-5
- Formula: C5H8N2O5
- Molecular Weight:176.13
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
All Endogenous Metabolite Isoforms
More
Biological Activity
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Male B6D2Fl mice were subcutaneously injected Lewis lung carcinoma cells[2]
-
Dosage:350 mg/kg
-
Administration:Subcutaneous injection; twice daily; for 18 days
-
Result:Inhibited antitumor activity and toxicity induced by N-phosphonacetyl-L-aspartate (PALA).
Chemical Information
-
CAS No. 923-37-5
-
Appearance Solid
-
Molecular Weight 176.13
-
Formula C5H8N2O5
-
Color White to off-white
-
SMILES
O=C(O)CC(C(O)=O)NC(N)=O
-
Synonyms
Ureidosuccinic Acid
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : 70 mg/mL (397.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (11.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (11.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (272 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. ANDERSON EP, et al. Ureidosuccinic acid as a precursor of nucleic acid pyrimidines in normal and tumor-bearing mice. J Biol Chem. 1955 Apr;213(2):625-33. [Content Brief]
[2]. Johnson RK. Reversal of toxicity and antitumor activity of N-(phosphonacetyl)-L-aspartate by uridine or carbamyl-DL-asparate in vivo. Biochem Pharmacol. 1977 Jan 1;26(1):81-4. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.6776 mL | 28.3881 mL | 56.7762 mL | 141.9406 mL |
| 5 mM | 1.1355 mL | 5.6776 mL | 11.3552 mL | 28.3881 mL | |
| 10 mM | 0.5678 mL | 2.8388 mL | 5.6776 mL | 14.1941 mL | |
| 15 mM | 0.3785 mL | 1.8925 mL | 3.7851 mL | 9.4627 mL | |
| 20 mM | 0.2839 mL | 1.4194 mL | 2.8388 mL | 7.0970 mL | |
| 25 mM | 0.2271 mL | 1.1355 mL | 2.2710 mL | 5.6776 mL | |
| 30 mM | 0.1893 mL | 0.9463 mL | 1.8925 mL | 4.7314 mL | |
| 40 mM | 0.1419 mL | 0.7097 mL | 1.4194 mL | 3.5485 mL | |
| 50 mM | 0.1136 mL | 0.5678 mL | 1.1355 mL | 2.8388 mL | |
| 60 mM | 0.0946 mL | 0.4731 mL | 0.9463 mL | 2.3657 mL | |
| 80 mM | 0.0710 mL | 0.3549 mL | 0.7097 mL | 1.7743 mL | |
| 100 mM | 0.0568 mL | 0.2839 mL | 0.5678 mL | 1.4194 mL |
N-Carbamoyl-DL-aspartic acid Related Classifications
HY-128425 Related Classifications
Powered by Bioz