L-Asparagine
Based on 6 publication(s) in Google Scholar
L-Asparagine is an essential amino acid for leukemic cells and a substrate for L-Asparaginase. L-Asparaginase is a potent anti-leukemic enzyme that promotes asparagine (Asn) and glutamine (Gln) depletion and inhibits protein biosynthesis in lymphoblasts. Removal of L-asparagine from plasma by L-Asparaginase results in inhibition of RNA and DNA synthesis and subsequent apoptosis. L-Asparaginase has cell-killing ability in vitro and in vivo, and selectively inhibits the growth of cancer cells with low asparagine synthetase (AASNS) expression. L-Asparagine can be used as a biomarker and sensor for the study of childhood acute lymphoblastic leukemia.
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- Pureza : 98.0%
- No. CAS: 70-47-3
- Fòrmula: C4H8N2O3
- Peso molecular:132.12
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Publications Citing Use of MedChemExpress (MCE) L-Asparagine
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In Vitro
L-Asparagine is the biological substrate of L-Asparaginase. The potential side effects of L-Asparaginase are causing normal cell death and inducing inflammation, metabolic disorders and neurotoxicity after use. The toxic side effects of L-Asparaginase may be caused by the action of glutaminase in it[1].
L-Asparagine and L-Asparaginase have also been used to develop biosensors for analyzing asparagine levels in leukemia or food industry. In application, the characteristics of L-Asparagine hydrolysis, which produces ammonium ions and causes pH changes, can be used to determine the level of L-Asparagine by color and absorbance changes[1].
L-Asparaginase can preferentially kill leukemia cells, and the leukemia cell killing rate can be accurately measured using leukemia cell surface markers. L-Asparaginase showed no significant dose response at doses of 0.0001-0.1 IU/mL[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
In the NOD.Cg-PRKDCscidIL2RGtm1Wjl (NSG) mouse model, L-Asparaginase (1000 or 5000 IU/kg; intraperitoneal injection; once a day for 3 consecutive days) significantly depletes L-Asparagine in the blood; in the high-dose group, L-Asparagine also recoveres slowly after the cessation of L-Asparaginase injection[3].
When L-Asparaginase is encapsulated in red blood cells (ASP-RBC) and given to Swiss mice by intravenous injection (200 or 1000 IU/kg), the depletion time of L-Asparagine in their plasma is prolonged. In the 1000 IU/kg dose group, levels of L-Asparagine remains undetectable for up to 14 days, and the half-life of the enzyme is 9-10 days[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 70-47-3
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Appearance Solid
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Peso molecular 132.12
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Fòrmula C4H8N2O3
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Color White to off-white
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SMILES
N[C@H](C(O)=O)CC(N)=O
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Synonyms
(-)-Asparagine; Asn; Asparamide
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (6)
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Journal Impact Factor
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Most Recent
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Acta Pharm Sin B
Inhibition of ASCT2 induces hepatic stellate cell senescence with modified proinflammatory secretome through an IL-1 α/NF- κ B feedback pathway to inhibit liver fibrosis. [Abstract]2022 Sep;12(9):3618-3638. PMID: 36176909 -
Commun Biol
Elevated glutamate impedes anti-HIV-1 CD8 + T cell responses in HIV-1-infected individuals on antiretroviral therapy. [Abstract]2023 Jul 7;6(1):696. PMID: 37419968 -
Int Dent J
Tooth Loss-Induced Gut Dysbiosis Promotes Neuroinflammation via L-Asparagine-Mediated Neuronal Toxicity. [Abstract]2025 Oct 1;75(6):103929. PMID: 41043307
L-Asparagine purchased from MedChemExpress. Usage Cited in: Int Dent J. 2025 Oct 1;75(6):103929. [Abstract]
L-Asparagine (LA) (1 mM; 48 h) increased the percentage of apoptotic HT22 cells.
L-Asparagine purchased from MedChemExpress. Usage Cited in: Int Dent J. 2025 Oct 1;75(6):103929. [Abstract]
viability of HT22 cells was assessed by CCK-8 assay at 0, 6, 12, 24, and 48 hours after L-Asparagine (LA) (1 mM; 48 h) treatment.
L-Asparagine purchased from MedChemExpress. Usage Cited in: Int Dent J. 2025 Oct 1;75(6):103929. [Abstract]
L-Asparagine (LA) (1 mM; 48 h) increased intracellular ROS levels of HT22 cells.
L-Asparagine purchased from MedChemExpress. Usage Cited in: Int Dent J. 2025 Oct 1;75(6):103929. [Abstract]
L-Asparagine (LA) (1 mM; 48 h) enhanced expression of TNF-α in HT22 cells, implicating an inflammatory response.
L-Asparagine purchased from MedChemExpress. Usage Cited in: Int Dent J. 2025 Oct 1;75(6):103929. [Abstract]
L-Asparagine (LA) (1 mM; 48 h) treatment increased the fluorescence intensity of p-Akt and p-NF-κB in HT22 cells, while total Akt and NF-κB levels remained unchanged.
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Amino Acids
Identification of amino acids metabolomic profiling in human plasma distinguishes lupus nephritis from systemic lupus erythematosus. [Abstract]2024 Sep 18;56(1):56. PMID: 39292313 -
Solvente y solubilidad
In Vitro:
H2O : 6.67 mg/mL (50.48 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 10 mg/mL (75.69 mM); Clear solution; Need ultrasonic
Pureza y Documentación
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Ficha de datos (274 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Batool T, et al. A Comprehensive Review on L-Asparaginase and Its Applications. Appl Biochem Biotechnol. 2016 Mar;178(5):900-23. [Content Brief]
[2]. Asselin BL, et al. In vitro and in vivo killing of acute lymphoblastic leukemia cells by L-asparaginase. Cancer Res. 1989 Aug 1;49(15):4363-8. [Content Brief]
[3]. Horvath TD, et al. Assessment of l-Asparaginase Pharmacodynamics in Mouse Models of Cancer. Metabolites. 2019 Jan 9;9(1):10. [Content Brief]
[4]. Kravtzoff R, et al. Erythrocytes as carriers for L-asparaginase. Methodological and mouse in-vivo studies. J Pharm Pharmacol. 1990 Jul;42(7):473-6. [Content Brief]
[5]. Ilaria M Michelozzi, et al. Acute myeloid leukaemia niche regulates response to L-asparaginase. Br J Haematol. 2019 Aug;186(3):420-430. doi: 10.1111/bjh.15920. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 7.5689 mL | 37.8444 mL | 75.6888 mL | 189.2219 mL |
| 5 mM | 1.5138 mL | 7.5689 mL | 15.1378 mL | 37.8444 mL | |
| 10 mM | 0.7569 mL | 3.7844 mL | 7.5689 mL | 18.9222 mL | |
| 15 mM | 0.5046 mL | 2.5230 mL | 5.0459 mL | 12.6148 mL | |
| 20 mM | 0.3784 mL | 1.8922 mL | 3.7844 mL | 9.4611 mL | |
| 25 mM | 0.3028 mL | 1.5138 mL | 3.0276 mL | 7.5689 mL | |
| 30 mM | 0.2523 mL | 1.2615 mL | 2.5230 mL | 6.3074 mL | |
| 40 mM | 0.1892 mL | 0.9461 mL | 1.8922 mL | 4.7305 mL | |
| 50 mM | 0.1514 mL | 0.7569 mL | 1.5138 mL | 3.7844 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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