L-Asparagine-13C4,15N2,d3 monohydrate
Based on 1 Customer Validation
L-Asparagine-13C4,15N2,d3 monohydrate is the deuterium, 13C-, and 15N-labeled L-Asparagine monohydrate (HY-W017443). L-Asparagine monohydrate is an essential amino acid for leukemic cells and a substrate for L-Asparaginase. L-Asparaginase is a potent anti-leukemic enzyme that promotes asparagine (Asn) and glutamine (Gln) depletion and inhibits protein biosynthesis in lymphoblasts. Removal of L-asparagine from plasma by L-Asparaginase results in inhibition of RNA and DNA synthesis and subsequent apoptosis. L-Asparaginase has cell-killing ability in vitro and in vivo, and selectively inhibits the growth of cancer cells with low asparagine synthetase (AASNS) expression. L-Asparagine monohydrate can be used as a biomarker and sensor for the study of childhood acute lymphoblastic leukemia.
For research use only. We do not sell to patients.
- Purity : 99.90%
- CAS No.: 2483829-30-5
- Formula: 13C4H7D3 15N2O4
- Molecular Weight:159.11
-
Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
In Vitro
Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Application
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
-
CAS No. 2483829-30-5
-
Unlabeled CAS 70-47-3
-
Appearance Solid
-
Molecular Weight 159.11
-
Formula 13C4H7D3 15N2O4
-
Color White to off-white
-
SMILES
[15NH2][13C]([13C]([2H])([2H])[13C@@]([15NH2])([2H])[13C](O)=O)=O.O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
In Vitro:
H2O : 20 mg/mL (125.70 mM; Need ultrasonic and warming)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (278 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019;53(2):211-236. [Content Brief]
[2]. Batool T, et al. A Comprehensive Review on L-Asparaginase and Its Applications. Appl Biochem Biotechnol. 2016 Mar;178(5):900-23. [Content Brief]
[3]. Asselin BL, et al. In vitro and in vivo killing of acute lymphoblastic leukemia cells by L-asparaginase. Cancer Res. 1989 Aug 1;49(15):4363-8. [Content Brief]
[4]. Horvath TD, et al. Assessment of l-Asparaginase Pharmacodynamics in Mouse Models of Cancer. Metabolites. 2019 Jan 9;9(1):10. [Content Brief]
[5]. Kravtzoff R, et al. Erythrocytes as carriers for L-asparaginase. Methodological and mouse in-vivo studies. J Pharm Pharmacol. 1990 Jul;42(7):473-6. [Content Brief]
[6]. Ilaria M Michelozzi, et al. Acute myeloid leukaemia niche regulates response to L-asparaginase. Br J Haematol. 2019 Aug;186(3):420-430. doi: 10.1111/bjh.15920. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 6.2850 mL | 31.4248 mL | 62.8496 mL | 157.1240 mL |
| 5 mM | 1.2570 mL | 6.2850 mL | 12.5699 mL | 31.4248 mL | |
| 10 mM | 0.6285 mL | 3.1425 mL | 6.2850 mL | 15.7124 mL | |
| 15 mM | 0.4190 mL | 2.0950 mL | 4.1900 mL | 10.4749 mL | |
| 20 mM | 0.3142 mL | 1.5712 mL | 3.1425 mL | 7.8562 mL | |
| 25 mM | 0.2514 mL | 1.2570 mL | 2.5140 mL | 6.2850 mL | |
| 30 mM | 0.2095 mL | 1.0475 mL | 2.0950 mL | 5.2375 mL | |
| 40 mM | 0.1571 mL | 0.7856 mL | 1.5712 mL | 3.9281 mL | |
| 50 mM | 0.1257 mL | 0.6285 mL | 1.2570 mL | 3.1425 mL | |
| 60 mM | 0.1047 mL | 0.5237 mL | 1.0475 mL | 2.6187 mL | |
| 80 mM | 0.0786 mL | 0.3928 mL | 0.7856 mL | 1.9641 mL | |
| 100 mM | 0.0628 mL | 0.3142 mL | 0.6285 mL | 1.5712 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
L-Asparagine-13C4,15N2,d3 monohydrate Related Classifications
HY-W017443S2 Related Classifications
Powered by Bioz