2. Isotope-Labeled Compounds
  3. Research Area
  4. SILAC Reagents

SILAC Reagents

Stable isotope labeling by amino acids in cell culture (SILAC) is the most commonly used method for quantitative proteomic analysis, thanks to its simplicity and high reproducibility. It was first used in quantitative proteomics by Ong et al. from the Mann Laboratory in Denmark in 2022. It can provide accurate relative quantification without any chemical derivatization or other operations, and is widely used in research on cell biology, biochemistry, pharmacology, etc [1][2].

Workflow for SILAC experiments

 

Figure 1. Workflow for SILAC

 

References:

[1] Nat Protoc. 2006; 1(6):2650-60.

[2] Proteomics. 2015 Sep; 15(18):3175-92.

SILAC Reagents (539):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-I1124
    L-Valine-d8 35045-72-8 99.10%
    L-Valine-d8 is a deuterated form of L-Valine. L-Valine-d8 can be used in the labelled synthesis of L-valineamide-d8 intermediate[1]. L-Valine is one of 20 proteinogenic amino acids. L-Valine is an essential amino acid[2].
    L-Valine-d<sub>8</sub>
  • HY-N0067S
    γ-Aminobutyric acid-d6 70607-85-1 99.12%
    γ-Aminobutyric acid-d6 is the deuterium labeled γ-Aminobutyric acid. γ-Aminobutyric acid (4-Aminobutyric acid) is a major inhibitory neurotransmitter in the adult mammalian brain[1][2], binding to the ionotropic GABA receptors (GABAA receptors) and metabotropic receptors (GABAB receptors)[2].
    γ-Aminobutyric acid-d<sub>6</sub>
  • HY-N0215S12
    L-Phenylalanine-d5 56253-90-8 99.15%
    L-Phenylalanine-d5 is the deuterium labeled L-Phenylalanine. L-Phenylalanine ((S)-2-Amino-3-phenylpropionic acid) is an essential amino acid isolated from Escherichia coli. L-Phenylalanine is a α2δ subunit of voltage-dependent Ca+ channels antagonist with a Ki of 980 nM. L-phenylalanine is a competitive antagonist for the glycine- and glutamate-binding sites of N-methyl-D-aspartate receptors (NMDARs) (KB of 573 μM ) and non-NMDARs, respectively. L-Phenylalanine is widely used in the production of food flavors and pharmaceuticals[1][2][3][4].
    L-Phenylalanine-d<sub>5</sub>
  • HY-N0623S
    L-Tryptophan-d5 62595-11-3 99.85%
    L-Tryptophan-d5 is the deuterium labeled L-Tryptophan. L-Tryptophan (Tryptophan) is an essential amino acid that is the precursor of serotonin, melatonin, and vitamin B3[1].
    L-Tryptophan-d<sub>5</sub>
  • HY-N0486S1
    L-Leucine-13C 74292-94-7 ≥98.00%
    L-Leucine-13C is the 13C-labeled L-Leucine. L-Leucine is an essential branched-chain amino acid (BCAA), which activates the mTOR signaling pathway[1].
    L-Leucine-<sup>13</sup>C
  • HY-N0486S9
    L-Leucine-d3 87828-86-2 ≥98.0%
    L-Leucine-d3 is the deuterium labeled L-Leucine. L-Leucine is an essential branched-chain amino acid (BCAA), which activates the mTOR signaling pathway[1].
    L-Leucine-d<sub>3</sub>
  • HY-N0390S9
    L-Glutamine-15N-1 59681-32-2 ≥98.0%
    L-Glutamine-15N-1 is the 15N-labeled L-Glutamine. L-Glutamine (L-Glutamic acid 5-amide) is a non-essential amino acid present abundantly throughout the body and involved in many metabolic processes. L-Glutamine provides a source of carbons for oxidation in some cells[1][2].
    L-Glutamine-<sup>15</sup>N-1
  • HY-W010347S
    DL-Homocysteine-d4 416845-90-4 ≥98.0%
    DL-Homocysteine-d4 is the deuterium labeled DL-Homocysteine. DL-Homocysteine is a weak neurotoxin, and can affect the production of kynurenic acid in the brain[1][2].
    DL-Homocysteine-d<sub>4</sub>
  • HY-N0229S3
    L-Alanine-d4 18806-29-6 99.97%
    L-Alanine-d4 is the deuterium labeled L-Alanine. L-Alanine is a non-essential amino acid, involved in sugar and acid metabolism, increases immunity, and provides energy for muscle tissue, brain, and central nervous system.
    L-Alanine-d<sub>4</sub>
  • HY-N0455AS8
    L-Arginine-13C6,15N4 hydrochloride 202468-25-5 ≥99.0%
    L-Arginine-13C6,15N4 (hydrochloride) is the 13C- and 15N-labeled L-Arginine hydrochloride. L-Arginine hydrochloride ((S)-(+)-Arginine hydrochloride) is the nitrogen donor for synthesis of nitric oxide, a potent vasodilator that is deficient during times of sickle cell crisis.
    L-Arginine-<sup>13</sup>C<sub>6</sub>,<sup>15</sup>N<sub>4</sub> hydrochloride
  • HY-N0771S3
    L-Isoleucine-d10 35045-71-7 ≥98.0%
    L-Isoleucine-d10 is the deuterium labeled L-Isoleucine. L-isoleucine is a nonpolar hydrophobic amino acid[1]. L-Isoleucine is an essential amino acid.
    L-Isoleucine-d<sub>10</sub>
  • HY-N0390S5
    L-Glutamine-1-13C 159663-16-8 ≥98.0%
    L-Glutamine-1-13C is the 13C-labeled L-Glutamine. L-Glutamine (L-Glutamic acid 5-amide) is a non-essential amino acid present abundantly throughout the body and involved in many metabolic processes. L-Glutamine provides a source of carbons for oxidation in some cells[1][2].
    L-Glutamine-1-<sup>13</sup>C
  • HY-Y0252S
    L-Proline-13C5 201740-83-2 99.00%
    L-Proline-13C5 is the 13C-labeled L-Proline. L-Proline is one of the twenty amino acids used in living organisms as the building blocks of proteins.
    L-Proline-<sup>13</sup>C<sub>5</sub>
  • HY-D0187S
    L-Glutathione reduced-13C2,15N 815610-65-2
    L-Glutathione reduced-13C2,15N is the 13C- and 15N-labeled L-Glutathione reduced. L-Glutathione reduced (GSH) is an endogenous antioxidant and is capable of scavenging oxygen-derived free radicals.
    L-Glutathione reduced-<sup>13</sup>C<sub>2</sub>,<sup>15</sup>N
  • HY-N0326S7
    L-Methionine-d3 13010-53-2 ≥98.0%
    L-Methionine-d3 is the deuterium labeled L-Methionine. L-Methionine is the L-isomer of Methionine, an essential amino acid for human development. Methionine acts as a hepatoprotectant.
    L-Methionine-d<sub>3</sub>
  • HY-N0390S8
    L-Glutamine-15N2 204451-48-9 ≥98.0%
    L-Glutamine-15N2 is the 15N-labeled L-Glutamine. L-Glutamine (L-Glutamic acid 5-amide) is a non-essential amino acid present abundantly throughout the body and involved in many metabolic processes. L-Glutamine provides a source of carbons for oxidation in some cells[1][2].
    L-Glutamine-<sup>15</sup>N<sub>2</sub>
  • HY-N0650S
    L-Serine-13C3 201595-68-8 98.20%
    L-Serine-13C3 is the 13C-labeled L-Serine. L-Serine ((-)-Serine; (S)-Serine), one of the so-called non-essential amino acids, plays a central role in cellular proliferation.
    L-Serine-<sup>13</sup>C<sub>3</sub>
  • HY-N0666S8
    L-Aspartic acid-d3 3842-25-9 ≥99.0%
    L-Aspartic acid-d3 is the deuterium labeled L-Aspartic acid. L-Aspartic acid is is an amino acid, shown to be a suitable proagent for colon-specific agent deliverly.
    L-Aspartic acid-d<sub>3</sub>
  • HY-N0717S6
    L-Valine-13C5 55443-52-2 ≥98.0%
    L-Valine-13C5 is the 13C-labeled L-Valine. L-Valine is one of 20 proteinogenic amino acids. L-Valine is an essential amino acid[1].
    L-Valine-<sup>13</sup>C<sub>5</sub>
  • HY-N0486S3
    L-Leucine-15N 59935-31-8 ≥98.0%
    L-Leucine-15N is the 15N-labeled L-Leucine. L-Leucine is an essential branched-chain amino acid (BCAA), which activates the mTOR signaling pathway[1][2].
    L-Leucine-<sup>15</sup>N