SILAC Reagents
Stable isotope labeling by amino acids in cell culture (SILAC) is the most commonly used method for quantitative proteomic analysis, thanks to its simplicity and high reproducibility. It was first used in quantitative proteomics by Ong et al. from the Mann Laboratory in Denmark in 2022. It can provide accurate relative quantification without any chemical derivatization or other operations, and is widely used in research on cell biology, biochemistry, pharmacology, etc [1][2].
Workflow for SILAC experiments
Figure 1. Workflow for SILAC
References:
[1] Nat Protoc. 2006; 1(6):2650-60.
[2] Proteomics. 2015 Sep; 15(18):3175-92.
- Formula: C14H14D5NO4
- Molecular Weight: 270.34
BOC-L-phenylalanine-d5 is the deuterium labeled BOC-L-phenylalanine (HY-79096). Boc-L-phenylalanine is a Boc-protected L-phenylalanine that can be used in the synthesis of other active compounds. Boc-L-phenylalanine is also a capping agent.
- Formula: C3H6DNO2
- Molecular Weight: 90.10
D-Alanine-d is the deuterium labeled D-Alanine. D-Alanine is a weak GlyR (inhibitory glycine receptor) and PMBA agonist, with an EC50 of 9 mM for GlyR.
- Formula: C8H11D4NO4
- Molecular Weight: 193.23
Boc-L-Ala-OH-d4 is the deuterium labeled Boc-L-Ala-OH.
- Formula: C7H13D3NO6P
- Molecular Weight: 244.20
N-Acetyl-DL-glufosinate-d3 (hydrate) is the deuterium labeled N-Acetyl-DL-glufosinate.
- Formula: C8H8D7NO3S
- Molecular Weight: 212.32
N-Acetyl-S-propyl-L-cysteine-d7 is the deuterium labeled N-Acetyl-S-propyl-L-cysteine.
- Formula: C313C2H13ClN2O2
- Molecular Weight: 170.61
L-Ornithine-1,2-13C2 hydrochloride is the 13C-labeled L-Ornithine hydrochloride (HY-W017018). L-Ornithine hydrochloride is an orally active CaSR and p38 activator. L-Ornithine hydrochloride inhibits ROS and supports urea cycle function. L-Ornithine hydrochloride can be used to study kidney diseases involving proximal tubular cell damage caused by oxidative stress or crystallopathy, as well as research related to anxiety disorders.
- Formula: C6H12Cl15N3O3
- Molecular Weight: 212.61
L-Histidine-15N3 hydrochloride hydrate is the 15N-labeled L-Histidine hydrochloride hydrate (HY-W014423). L-Histidine hydrochloride hydrate is an endogenous metabolite. L-Histidine hydrochloride hydrate scavenges hydroxyl radicals and singlet oxygen, regulate the absorption of zinc, copper and iron, exhibits anti-inflammatory and antioxidant activities. L-Histidine hydrochloride hydrate is blood brain barrier penetrable.
- Formula: 13C6H7D5Cl15N3O3
- Molecular Weight: 223.60
L-Histidine-13C6,15N3,d5 hydrochloride hydrate is the deuterium, 13C-, and 15-labeled L-Histidine hydrochloride hydrate (HY-W014423). L-Histidine hydrochloride hydrate is an endogenous metabolite. L-Histidine hydrochloride hydrate scavenges hydroxyl radicals and singlet oxygen, regulate the absorption of zinc, copper and iron, exhibits anti-inflammatory and antioxidant activities. L-Histidine hydrochloride hydrate is blood brain barrier penetrable.
- Formula: C413CH1115NO2
- Molecular Weight: 119.13
L-Valine-1-13C,15N ((S)-Valine-1-13C,15N) is the 13C- and 15N-labeled L-Valine (HY-N0717). L-Valine ((S)-Valine) is a nonlinear semiorganic material. L-Valine causes lipid peroxidation and accumulation of malondialdehyde (MDA), exhibits inhibitory activity against cyanobacteria. L-Valine inhibits multidrug-resistant bacteria through activation of PI3K/Akt signaling pathway and inhibition of arginase.
- Formula: C3H4D315NO3
- Molecular Weight: 109.10
L-Serine-15N,d3 is the deuterium and 15N-labeled L-Serine. L-Serine ((-)-Serine; (S)-Serine), one of the so-called non-essential amino acids, plays a central role in cellular proliferation.
- Formula: C11H4D815N2O2
- Molecular Weight: 214.26
L-Tryptophan-15N2,d8 is the deuterium and 15N-labeled L-Tryptophan. L-Tryptophan (Tryptophan) is an essential amino acid that is the precursor of serotonin, melatonin, and vitamin B3.
- Formula: C6H8D7Cl15N4O2
- Molecular Weight: 221.68
L-Arginine-15N4,d7 (hydrochloride) is the deuterium and 15N-labeled L-Arginine hydrochloride. L-Arginine hydrochloride ((S)-(+)-Arginine hydrochloride) is the nitrogen donor for synthesis of nitric oxide, a potent vasodilator that is deficient during times of sickle cell crisis.
- Formula: C9H1115NO2
- Molecular Weight: 166.18
DL-3-Phenylalanine-15N is the 15N labeled DL-3-Phenylalanine.
- Formula: C813C3H19NO9
- Molecular Weight: 312.25
N-Acetyl-α-neuraminic acid-13C3 is the 13C labeled N-Acetyl-α-neuraminic acid.
