L-Ornithine hydrochloride
Based on 9 publication(s) in Google Scholar
L-Ornithine hydrochloride is an orally active CaSR and p38 activator. L-Ornithine hydrochloride inhibits ROS and supports urea cycle function. L-Ornithine hydrochloride can be used to study kidney diseases involving proximal tubular cell damage caused by oxidative stress or crystallopathy, as well as research related to anxiety disorders.
For research use only. We do not sell to patients.
- Purity: 99.78%
- CAS No.: 3184-13-2
- Formula: C5H13ClN2O2
- Molecular Weight:168.62
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) L-Ornithine hydrochloride
More- Nature. 2025 Jul;643(8070):192-200. [Abstract]
- Information Processing in Agriculture. 2026 Mar 3.
- PLoS Biol. 2024 Jun 27;22(6):e3002672. [Abstract]
- Molecules. 2025 Mar 9;30(6):1224. [Abstract]
- Molecules. 2023 Apr 11;28(8):3375. [Abstract]
- Amino Acids. 2024 Sep 18;56(1):56. [Abstract]
- J Cardiovasc Transl Res. 2025 Jun;18(3):459-470. [Abstract]
- ChemistrySelect. 2023 Jan 12.
- bioRxiv. 2024 Apr 3:2023.06.02.542933. [Abstract]
Customer Validation & Images
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ELISA
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WB
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | EC50 |
237 μM
Compound: 76654
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Substrate uptake by the Cationic Amino Acid Transporter (CAT-3, SLC7A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC7A3 cells
Substrate uptake by the Cationic Amino Acid Transporter (CAT-3, SLC7A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC7A3 cells
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10.5281/zenodo.6782708 |
| HEK293 | EC50 |
237 μM
Compound: 76654
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Substrate uptake by the Cationic Amino Acid Transporter (CAT-3, SLC7A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC7A3 cells (PubChem AID: 1745863)
Substrate uptake by the Cationic Amino Acid Transporter (CAT-3, SLC7A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC7A3 cells (PubChem AID: 1745863)
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10.5281/zenodo.6782708 |
L-ornithine (1-10 mM) monohydrochloride , suitable for cell culture, activates the calcium-sensing receptor (CaSR) in HK-2 cells in a concentration-dependent manner, leading to an increase in intracellular calcium ([Ca2+]i) levels, and this increase is inhibited by CaSR-specific siRNA[1].
L-ornithine (100 μM; 60 min) monohydrochloride, suitable for cell culture, activates p38 MAPK in HK-2 cells via the ROCE pathway, and this activation mediates the protective effect against H2O2-induced apoptosis[1].
L-ornithine (100-300 μM) monohydrochloride, suitable for cell culture, protects HK-2 cells against damage induced by H2O2-mediated ROS production and excessive Ca2+ influx via the ROCE pathway[1].
L-ornithine monohydrochloride, suitable for cell culture, protects HK-2 cells against crystal-induced nuclear damage and H2O2-induced apoptosis/necrosis via the ROCE pathway[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human kidney 2 (HK-2) proximal tubular cells
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Concentration:100 μM (p38 phosphorylation measurement)
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Incubation Time:60 min (p38 phosphorylation measurement)
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Result:Optimally activated p38 phosphorylation after 60 min of exposure (P = 0.034).
Markedly reduced L-ornithine-induced p38 activation with inhibition of ROCE by Pyr10 (P = 0.005).
Showed no effect on basal p38 phosphorylation with H2O2 alone (P = 0.237) and Pyr10 alone (P = 0.761).
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 3184-13-2
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Appearance Solid
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Molecular Weight 168.62
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Formula C5H13ClN2O2
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Color White to off-white
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SMILES
NCCC[C@@](N)([H])C(O)=O.Cl
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (9)
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Journal Impact Factor
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Most Recent
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Nature
2025 Jul;643(8070):192-200. PMID: 39695227 -
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PLoS Biol
2024 Jun 27;22(6):e3002672. PMID: 38935621 -
Molecules
Seeking Correlation Among Porin Permeabilities and Minimum Inhibitory Concentrations Through Machine Learning: A Promising Route to the Essential Molecular Descriptors. [Abstract]2025 Mar 9;30(6):1224. PMID: 40142001 -
Molecules
Untargeted Metabolomics Using UHPLC-HRMS Reveals Metabolic Changes of Fresh-Cut Potato during Browning Process. [Abstract]2023 Apr 11;28(8):3375. PMID: 37110608 -
Amino Acids
Identification of amino acids metabolomic profiling in human plasma distinguishes lupus nephritis from systemic lupus erythematosus. [Abstract]2024 Sep 18;56(1):56. PMID: 39292313 -
J Cardiovasc Transl Res
LACC1 Enhances Polyamine Immunometabolism in Inflammatory Macrophages to Inhibit Atherosclerosis Progression. [Abstract]2025 Jun;18(3):459-470. PMID: 40293654
L-Ornithine hydrochloride purchased from MedChemExpress. Usage Cited in: J Cardiovasc Transl Res. 2025 Jun;18(3):459-470. [Abstract]
After treating macrophages with siLACC1 and L-ornithine (Orn, 1 nM, 48 h), ELISA was utilized to detect proinflammatory factors in macrophages.
L-Ornithine hydrochloride purchased from MedChemExpress. Usage Cited in: J Cardiovasc Transl Res. 2025 Jun;18(3):459-470. [Abstract]
After treating macrophages with siLACC1 and L-ornithine (Orn, 1 nM, 48 h), Western blot was utilized to measure the expression of proinflammatory factors in macrophages.
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bioRxiv
An efficient behavioral screening platform classifies natural products and other chemical cues according to their chemosensory valence in C. elegans. [Abstract]2024 Apr 3:2023.06.02.542933. PMID: 37333363
Solvent & Solubility
H2O : 50 mg/mL (296.52 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (593.05 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Shin S, et al. l-ornithine activates Ca signaling to exert its protective function on human proximal tubular cells. Cellular signalling. 2020 Mar;67:109484. [Content Brief]
[2]. Demura S, et al. Effect of L-ornithine hydrochloride ingestion on intermittent maximal anaerobic cycle ergometer performance and fatigue recovery after exercise. European journal of applied physiology. 2011 Nov;111(11):2837-43. [Content Brief]
[3]. Kurata K, et al. Orally administered L-ornithine elevates brain L-ornithine levels and has an anxiolytic-like effect in mice[J]. Nutritional Neuroscience, 2011, 14(6): 243-248. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 5.9305 mL | 29.6525 mL | 59.3049 mL | 148.2624 mL |
| 5 mM | 1.1861 mL | 5.9305 mL | 11.8610 mL | 29.6525 mL | |
| 10 mM | 0.5930 mL | 2.9652 mL | 5.9305 mL | 14.8262 mL | |
| 15 mM | 0.3954 mL | 1.9768 mL | 3.9537 mL | 9.8842 mL | |
| 20 mM | 0.2965 mL | 1.4826 mL | 2.9652 mL | 7.4131 mL | |
| 25 mM | 0.2372 mL | 1.1861 mL | 2.3722 mL | 5.9305 mL | |
| 30 mM | 0.1977 mL | 0.9884 mL | 1.9768 mL | 4.9421 mL | |
| 40 mM | 0.1483 mL | 0.7413 mL | 1.4826 mL | 3.7066 mL | |
| 50 mM | 0.1186 mL | 0.5930 mL | 1.1861 mL | 2.9652 mL | |
| 60 mM | 0.0988 mL | 0.4942 mL | 0.9884 mL | 2.4710 mL | |
| 80 mM | 0.0741 mL | 0.3707 mL | 0.7413 mL | 1.8533 mL | |
| 100 mM | 0.0593 mL | 0.2965 mL | 0.5930 mL | 1.4826 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
L-Ornithine hydrochloride Related Classifications
HY-W017018 Related Classifications
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