2. Metabolic Enzyme/Protease Apoptosis NF-κB Immunology/Inflammation
  3. Endogenous Metabolite Apoptosis Reactive Oxygen Species (ROS)
  4. L-Histidine hydrochloride hydrate

L-Histidine hydrochloride hydrate  (Synonyms: H-His-OH.HCl.H2O)

Cat. No.: HY-W014423 Purity: 98.0%
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Handling Instructions Technical Support

L-Histidine hydrochloride hydrate is an endogenous metabolite. L-Histidine hydrochloride hydrate scavenges hydroxyl radicals and singlet oxygen, regulate the absorption of zinc, copper and iron, exhibits anti-inflammatory and antioxidant activities. L-Histidine hydrochloride hydrate is blood brain barrier penetrable.

For research use only. We do not sell to patients.

CAS No. : 5934-29-2

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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10 mM * 1 mL in Water In-stock
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100 mg In-stock
500 mg In-stock
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L-Histidine hydrochloride hydrate Related Antibodies

Top Publications Citing Use of Products
Cell Proliferation/Viability Assay
In Vivo Efficacy Study
2D/3D Cell Culture and Differentiation

    L-Histidine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8.  [Abstract]

    Cell viability on day 4 at OD570 of CRC cells treated with different concentrations of CRC-depleted L-histidine (0.5-10 mM).

    L-Histidine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8.  [Abstract]

    Experimental schematic and colonoscopy images. L-histidine (100 mM; 100 μL; i.g.; every other day) reduced tumor size and cell proliferation in ApcMin/+ mice.

    L-Histidine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8.  [Abstract]

    Colony formation assays after L-histidine (5 mM; 10 d) treatment.

    L-Histidine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8.  [Abstract]

    Cell viability at OD570 after L-histidine (5 mM; dissolved in PBS with 10% DMSO; 5 d) treatment.

    L-Histidine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8.  [Abstract]

    Colony formation assays after L-histidine (10 d) treatment.

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    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    L-Histidine hydrochloride hydrate is an endogenous metabolite. L-Histidine hydrochloride hydrate scavenges hydroxyl radicals and singlet oxygen, regulate the absorption of zinc, copper and iron, exhibits anti-inflammatory and antioxidant activities. L-Histidine hydrochloride hydrate is blood brain barrier penetrable[1][2][3].

    In Vitro

    L-Histidine hydrochloride hydrate (1 mM, 20 h) enhances the cytotoxicity of H2O2, induces apoptosis by inducing DNA double-strand breaks[1].
    L-Histidine hydrochloride hydrate (0.2-10 mM, 20 h) reduces zinc-induced GT1-7 cell death (ED50=0.2 mM), downregulates the expression of zinc-induced ER stress-related genes (such as GADD45, GADD34 and p8) and calcium homeostasis-related genes (such as Arc)[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    L-Histidine hydrochloride hydrate Related Antibodies

    Apoptosis Analysis[1]

    Cell Line: U937
    Concentration: 1 mM
    Incubation Time: 20 h
    Result: Induced apoptosis.
    In Vivo

    L-Histidine hydrochloride hydrate (250 mg/kg, intra-rectal, 3 times a day for 5 days) exhibits anti-inflammatory efficacy in Acetic acid (HY-Y0319)-induced rats ulcerative colitis models[3].
    L-Histidine hydrochloride hydrate (250 mg/kg, ip, single dose) exhibits neuroprotective efficacy in rat ischemia-reperfusion injury model[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Rats ulcerative colitis models[3]
    Dosage: 250 mg/kg
    Administration: intra-rectal administration, 3 times a day for 5 days
    Result: Reduced the severity of colitis, reduced myeloperoxidase activity in colonic tissue.
    Animal Model: Rat ischemia-reperfusion injury model[3]
    Dosage: 250 mg/kg
    Administration: ip, single dose
    Result: Reduced ischemia-induced neuronal death.
    Molecular Weight

    209.63

    Formula

    C6H12ClN3O3
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(O)[C@](N)([H])CC1=CN=CN1.Cl.O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Store at room temperature, keep dry and cool

    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (477.03 mM; Need ultrasonic)

    DMSO : 1 mg/mL (4.77 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.7703 mL 23.8515 mL 47.7031 mL
    5 mM 0.9541 mL 4.7703 mL 9.5406 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 50 mg/mL (238.52 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 98.0%

    SDS (393 KB)

    COA (252 KB) HNMR (254 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 4.7703 mL 23.8515 mL 47.7031 mL 119.2577 mL
    H2O 5 mM 0.9541 mL 4.7703 mL 9.5406 mL 23.8515 mL
    10 mM 0.4770 mL 2.3852 mL 4.7703 mL 11.9258 mL
    15 mM 0.3180 mL 1.5901 mL 3.1802 mL 7.9505 mL
    20 mM 0.2385 mL 1.1926 mL 2.3852 mL 5.9629 mL
    25 mM 0.1908 mL 0.9541 mL 1.9081 mL 4.7703 mL
    30 mM 0.1590 mL 0.7951 mL 1.5901 mL 3.9753 mL
    40 mM 0.1193 mL 0.5963 mL 1.1926 mL 2.9814 mL
    50 mM 0.0954 mL 0.4770 mL 0.9541 mL 2.3852 mL
    60 mM 0.0795 mL 0.3975 mL 0.7951 mL 1.9876 mL
    80 mM 0.0596 mL 0.2981 mL 0.5963 mL 1.4907 mL
    100 mM 0.0477 mL 0.2385 mL 0.4770 mL 1.1926 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    L-Histidine hydrochloride hydrate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    L-Histidine hydrochloride5934-29-2H-His-OH.HCl.H2OEndogenous MetaboliteApoptosisReactive Oxygen Species (ROS)Inhibitorinhibitorinhibit

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