Cat. No.: HY-N0832

L-Histidine

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

L-Histidine is an essential amino acid for infants. L-Histidine is an inhibitor of mitochondrial glutamine transport.

For research use only. We do not sell to patients.

Purity: 99.97%
CAS No.: 71-00-1
Formula: C₆H₉N₃O₂
Molecular Weight:155.15

Storage:

Store at room temperature 3 years.

In solvent -80°C, 1 year , -20°C, 6 months

L-Histidine Chemical Structure

CAS No.: 71-00-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

L-Histidine is an essential amino acid for infants. L-Histidine is an inhibitor of mitochondrial glutamine transport.

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

All Ligands for E3 Ligase Isoforms :

In Vitro

L-histidine completely inhibits growth and its effect on viability is inversely related to FLO11 expression. L-histidine does not affect the viability of the Δflo11 and S288c strains. L-histidine dramatically decreases air-liquid biofilm formation and adhesion to polystyrene of the flor yeasts with no effect on the transcription level of the FLO11 gene. Moreover, L-histidine modifies the chitin and glycans content on the cell-wall of flor yeasts^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

L-histidine (100 mg/kg) completely inhibits the brain edema in thioacetamide-treated rats^[2]. Histamine release stimulated by high K⁺ from the hypothalamus in the L-histidine diet group is 60% of that in the control group. However, the concentrations of other monoamines and their metabolites are not changed by the L-histidine diet. The open-field tests show that the L-histidine diet group spends a shorter amount of time in the central zone, and the light/dark box tests demonstrate that the L-histidine diet group spends a shorter amount of time in the light box, suggesting that the L-histidine diet induced anxiety-like behaviors^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 71-00-1
Appearance Solid
Molecular Weight 155.15
Formula C₆H₉N₃O₂
Color White to off-white
SMILES

N[C@@H](CC1=CNC=N1)C(O)=O
Structure Classification
- Amino acids
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	1 year
	-20°C	6 months

Publications (3)

All Cell Proliferation/Viability AssayIn Vivo Efficacy Study2D/3D Cell Culture and Differentiation

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Journal Impact Factor

Cancer Cell

Integrative plasma and fecal metabolomics identify functional metabolites in adenoma-colorectal cancer progression and as early diagnostic biomarkers. [Abstract]

2024 Aug 12;42(8):1386-1400.e8. PMID: 39137727

L-Histidine purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8. [Abstract]

Cell viability on day 4 at OD₅₇₀ of CRC cells treated with different concentrations of CRC-depleted L-histidine (0.5-10 mM).

L-Histidine purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8. [Abstract]

Experimental schematic and colonoscopy images. L-histidine (100 mM; 100 μL; i.g.; every other day) reduced tumor size and cell proliferation in Apc^Min/+ mice.

L-Histidine purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8. [Abstract]

Colony formation assays after L-histidine (5 mM; 10 d) treatment.

L-Histidine purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8. [Abstract]

Cell viability at OD₅₇₀ after L-histidine (5 mM; dissolved in PBS with 10% DMSO; 5 d) treatment.

L-Histidine purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8. [Abstract]

Colony formation assays after L-histidine (10 d) treatment.
Mol Med

Microbially produced imidazole propionate impairs prostate cancer progression through PDZK1. [Abstract]

2025 Jan 16;31(1):14. PMID: 39819421
Amino Acids

Identification of amino acids metabolomic profiling in human plasma distinguishes lupus nephritis from systemic lupus erythematosus. [Abstract]

2024 Sep 18;56(1):56. PMID: 39292313

Solvent & Solubility

In Vitro:

H₂O : 20.83 mg/mL (134.26 mM; ultrasonic and warming and heat to 80°C)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

6.4454 mL

32.2269 mL

64.4538 mL

5 mM

1.2891 mL

6.4454 mL

12.8908 mL

10 mM

0.6445 mL

3.2227 mL

6.4454 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 25 mg/mL (161.13 mM); Clear solution; Need ultrasonic

Protocol

Animal Administration^[2]^[3]

Rats: TAA (300 mg/kg i.p) is given to animals daily for 3 days. L-histidine (100 mg/kg) is dissolved in saline and injected (i.p.) daily 2 hours before each TAA injection. To prevent hypoglycemia and dehydration, rats are given 12.5 ml/kg of fluid therapy (5% dextrose and 0.45% saline with 20 mEq/L of potassium chloride) every 12 hours, s.c. Normal controls receive saline (vehicle used for TAA), whereas another group of rats are given L-histidine alone (100 mg/kg) daily for 3 days. TAA-treated rats are clinically monitored, and stages of encephalopathy are graded^[2].

Mice: The control group is fed with the AIN-93G purified diet that contains 5.08 g L-histidine/kg, whereas the L-histidine diet group is fed with AIN-93G that contains 1.28 g L-histidine/kg (25% of the histidine content in AIN-93G). To equalize the total amount of amino acids, glutamine is added to the L-histidine diet to counterbalance the changes in the histidine content (18.32 g L-glutamine/kg AIN-93G vs. 23.72 g L-glutamine/kg L-histidine diet). Both diets are isonitrogenous. At 8 wk of age, the mice are weighed and assigned to 2 different diets. The mice are allowed ad libitum access to water and their respective diets, and they are housed for at least 2 wk in the laboratory before starting the experiments^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bou Zeidan M, et al. L-histidine inhibits biofilm formation and FLO11-associated phenotypes in Saccharomyces cerevisiae flor yeasts. PLoS One. 2014 Nov 4;9(11):e112141. [Content Brief]

[2]. Rama Rao KV, et al. Brain edema in acute liver failure: inhibition by L-histidine. Am J Pathol. 2010 Mar;176(3):1400-8. [Content Brief]

[3]. Yoshikawa T, et al. Insufficient intake of L-histidine reduces brain histamine and causes anxiety-like behaviors in male mice. J Nutr. 2014 Oct;144(10):1637-41. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	6.4454 mL	32.2269 mL	64.4538 mL	161.1344 mL
	5 mM	1.2891 mL	6.4454 mL	12.8908 mL	32.2269 mL
	10 mM	0.6445 mL	3.2227 mL	6.4454 mL	16.1134 mL
	15 mM	0.4297 mL	2.1485 mL	4.2969 mL	10.7423 mL
	20 mM	0.3223 mL	1.6113 mL	3.2227 mL	8.0567 mL
	25 mM	0.2578 mL	1.2891 mL	2.5782 mL	6.4454 mL
	30 mM	0.2148 mL	1.0742 mL	2.1485 mL	5.3711 mL
	40 mM	0.1611 mL	0.8057 mL	1.6113 mL	4.0284 mL
	50 mM	0.1289 mL	0.6445 mL	1.2891 mL	3.2227 mL
	60 mM	0.1074 mL	0.5371 mL	1.0742 mL	2.6856 mL
	80 mM	0.0806 mL	0.4028 mL	0.8057 mL	2.0142 mL
	100 mM	0.0645 mL	0.3223 mL	0.6445 mL	1.6113 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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L-Histidine

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Publications Citing Use of MedChemExpress (MCE) L-Histidine

All Endogenous Metabolite Isoforms

All Ligands for E3 Ligase Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

L-Histidine Related Classifications

Cat. No.: HY-N0832