L-Hisidine (monohydrocholoride)

Name: L-Hisidine (monohydrocholoride)
Brand: MedChemExpress
SKU: HY-N0833
Rating: 5.0 (3 reviews)

Cat. No.: HY-N0833 Purity: 99.76%: Data Sheet
COA

SDS
Handling Instructions
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L-Hisidine is an essential amino acid for infants. L-Hisidine is an inhibitor of mitochondrial glutamine transport.

For research use only. We do not sell to patients.

CAS No. : 645-35-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
100 g	In-stock	Estimated Time of Arrival: December 31
250 g	In-stock	Estimated Time of Arrival: December 31
500 g	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of L-Hisidine (monohydrocholoride):

L-Histidine In-stock

3 Publications Citing Use of MCE L-Hisidine (monohydrocholoride)

Cell Proliferation/Viability Assay

In Vivo Efficacy Study

2D/3D Cell Culture and Differentiation

: L-Hisidine (monohydrocholoride) purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8. [Abstract]; Cell viability on day 4 at OD₅₇₀ of CRC cells treated with different concentrations of CRC-depleted L-histidine (0.5-10 mM).

: L-Hisidine (monohydrocholoride) purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8. [Abstract]; Experimental schematic and colonoscopy images. L-histidine (100 mM; 100 μL; i.g.; every other day) reduced tumor size and cell proliferation in Apc^Min/+ mice.

: L-Hisidine (monohydrocholoride) purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8. [Abstract]; Colony formation assays after L-histidine (5 mM; 10 d) treatment.

: L-Hisidine (monohydrocholoride) purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8. [Abstract]; Cell viability at OD₅₇₀ after L-histidine (5 mM; dissolved in PBS with 10% DMSO; 5 d) treatment.

: L-Hisidine (monohydrocholoride) purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8. [Abstract]; Colony formation assays after L-histidine (10 d) treatment.

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Customer Review

Description

L-Hisidine is an essential amino acid for infants. L-Hisidine is an inhibitor of mitochondrial glutamine transport.

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

In Vitro

L-histidine completely inhibits growth and its effect on viability is inversely related to FLO11 expression. L-histidine does not affect the viability of the Δflo11 and S288c strains. L-histidine dramatically decreases air-liquid biofilm formation and adhesion to polystyrene of the flor yeasts with no effect on the transcription level of the FLO11 gene. Moreover, L-histidine modifies the chitin and glycans content on the cell-wall of flor yeasts^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

L-histidine (100 mg/kg) completely inhibits the brain edema in thioacetamide-treated rats^[2]. Histamine release stimulated by high K⁺ from the hypothalamus in the L-histidine diet group is 60% of that in the control group. However, the concentrations of other monoamines and their metabolites are not changed by the L-histidine diet. The open-field tests show that the L-histidine diet group spends a shorter amount of time in the central zone, and the light/dark box tests demonstrate that the L-histidine diet group spends a shorter amount of time in the light box, suggesting that the L-histidine diet induced anxiety-like behaviors^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

191.62

Formula

C₆H₁₀ClN₃O₂

CAS No.

645-35-2

Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CC1=CNC=N1)C(O)=O.Cl

Structure Classification

Amino acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature, keep dry and cool

In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (260.93 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.2187 mL	26.0933 mL	52.1866 mL
5 mM	1.0437 mL	5.2187 mL	10.4373 mL
10 mM	0.5219 mL	2.6093 mL	5.2187 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.76%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (250 KB) HNMR (130 KB) RP-HPLC (221 KB)

Handling Instructions (2659 KB)

References

[1]. Bou Zeidan M, et al. L-histidine inhibits biofilm formation and FLO11-associated phenotypes in Saccharomyces cerevisiae flor yeasts. PLoS One. 2014 Nov 4;9(11):e112141. [Content Brief]

[2]. Rama Rao KV, et al. Brain edema in acute liver failure: inhibition by L-histidine. Am J Pathol. 2010 Mar;176(3):1400-8. [Content Brief]

[3]. Yoshikawa T, et al. Insufficient intake of L-histidine reduces brain histamine and causes anxiety-like behaviors in male mice. J Nutr. 2014 Oct;144(10):1637-41. [Content Brief]

Animal Administration
^[2]^[3]

Rats: TAA (300 mg/kg i.p) is given to animals daily for 3 days. L-histidine (100 mg/kg) is dissolved in saline and injected (i.p.) daily 2 hours before each TAA injection. To prevent hypoglycemia and dehydration, rats are given 12.5 ml/kg of fluid therapy (5% dextrose and 0.45% saline with 20 mEq/L of potassium chloride) every 12 hours, s.c. Normal controls receive saline (vehicle used for TAA), whereas another group of rats are given L-histidine alone (100 mg/kg) daily for 3 days. TAA-treated rats are clinically monitored, and stages of encephalopathy are graded^[2]. Mice: The control group is fed with the AIN-93G purified diet that contains 5.08 g L-histidine/kg, whereas the L-histidine diet group is fed with AIN-93G that contains 1.28 g L-histidine/kg (25% of the histidine content in AIN-93G). To equalize the total amount of amino acids, glutamine is added to the L-histidine diet to counterbalance the changes in the histidine content (18.32 g L-glutamine/kg AIN-93G vs. 23.72 g L-glutamine/kg L-histidine diet). Both diets are isonitrogenous. At 8 wk of age, the mice are weighed and assigned to 2 different diets. The mice are allowed ad libitum access to water and their respective diets, and they are housed for at least 2 wk in the laboratory before starting the experiments^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Bou Zeidan M, et al. L-histidine inhibits biofilm formation and FLO11-associated phenotypes in Saccharomyces cerevisiae flor yeasts. PLoS One. 2014 Nov 4;9(11):e112141. [Content Brief]

[2]. Rama Rao KV, et al. Brain edema in acute liver failure: inhibition by L-histidine. Am J Pathol. 2010 Mar;176(3):1400-8. [Content Brief]

[3]. Yoshikawa T, et al. Insufficient intake of L-histidine reduces brain histamine and causes anxiety-like behaviors in male mice. J Nutr. 2014 Oct;144(10):1637-41. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	5.2187 mL	26.0933 mL	52.1866 mL	130.4666 mL
	5 mM	1.0437 mL	5.2187 mL	10.4373 mL	26.0933 mL
	10 mM	0.5219 mL	2.6093 mL	5.2187 mL	13.0467 mL
	15 mM	0.3479 mL	1.7396 mL	3.4791 mL	8.6978 mL
	20 mM	0.2609 mL	1.3047 mL	2.6093 mL	6.5233 mL
	25 mM	0.2087 mL	1.0437 mL	2.0875 mL	5.2187 mL
	30 mM	0.1740 mL	0.8698 mL	1.7396 mL	4.3489 mL
	40 mM	0.1305 mL	0.6523 mL	1.3047 mL	3.2617 mL
	50 mM	0.1044 mL	0.5219 mL	1.0437 mL	2.6093 mL
	60 mM	0.0870 mL	0.4349 mL	0.8698 mL	2.1744 mL
	80 mM	0.0652 mL	0.3262 mL	0.6523 mL	1.6308 mL
	100 mM	0.0522 mL	0.2609 mL	0.5219 mL	1.3047 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.