L-Histidine-d5 hydrochloride hydrate
Based on 1 Customer Validation
L-Histidine-d5 hydrochloride hydrate is the deuterium labeled L-Histidine hydrochloride hydrate (HY-W014423). L-Histidine hydrochloride hydrate is an endogenous metabolite. L-Histidine hydrochloride hydrate scavenges hydroxyl radicals and singlet oxygen, regulate the absorption of zinc, copper and iron, exhibits anti-inflammatory and antioxidant activities. L-Histidine hydrochloride hydrate is blood brain barrier penetrable.
For research use only. We do not sell to patients.
- Purity : 99.96%
- CAS No.: 2483831-75-8
- Formula: C6H7D5ClN3O3
- Molecular Weight:214.66
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Application
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
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CAS No. 2483831-75-8
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Unlabeled CAS 5934-29-2
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Appearance Solid
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Molecular Weight 214.66
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Formula C6H7D5ClN3O3
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Color White to off-white
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SMILES
OC([C@](N)([2H])C([2H])([2H])C1=C([2H])N=C([2H])N1)=O.Cl.O
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Synonyms
H-His-OH-d5 hydrochloride hydrate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
In Vitro:
H2O : 125 mg/mL (582.32 mM; ultrasonic and adjust pH to 6 with NaOH)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Palomba L, et al., Low levels of hydrogen peroxide and L-histidine induce DNA double-strand breakage and apoptosis. Eur J Pharmacol. 1996 Dec 27;318(1):167-73. [Content Brief]
[2]. Kawahara M, et al., D-histidine and L-histidine attenuate zinc-induced neuronal death in GT1-7 cells. Metallomics. 2013 May;5(5):453-60. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 4.6585 mL | 23.2926 mL | 46.5853 mL | 116.4632 mL |
| 5 mM | 0.9317 mL | 4.6585 mL | 9.3171 mL | 23.2926 mL | |
| 10 mM | 0.4659 mL | 2.3293 mL | 4.6585 mL | 11.6463 mL | |
| 15 mM | 0.3106 mL | 1.5528 mL | 3.1057 mL | 7.7642 mL | |
| 20 mM | 0.2329 mL | 1.1646 mL | 2.3293 mL | 5.8232 mL | |
| 25 mM | 0.1863 mL | 0.9317 mL | 1.8634 mL | 4.6585 mL | |
| 30 mM | 0.1553 mL | 0.7764 mL | 1.5528 mL | 3.8821 mL | |
| 40 mM | 0.1165 mL | 0.5823 mL | 1.1646 mL | 2.9116 mL | |
| 50 mM | 0.0932 mL | 0.4659 mL | 0.9317 mL | 2.3293 mL | |
| 60 mM | 0.0776 mL | 0.3882 mL | 0.7764 mL | 1.9411 mL | |
| 80 mM | 0.0582 mL | 0.2912 mL | 0.5823 mL | 1.4558 mL | |
| 100 mM | 0.0466 mL | 0.2329 mL | 0.4659 mL | 1.1646 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
L-Histidine-d5 hydrochloride hydrate Related Classifications
HY-W014423S4 Related Classifications
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