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  4. Glycine

Glycine is an inhibitory neurotransmitter in the CNS and also acts as a co-agonist along with glutamate, facilitating an excitatory potential at the glutaminergic N-methyl-D-aspartic acid (NMDA) receptors. Glycine is orally active. Glycine inhibits the membrane aggregation of NINJ1 and prevents plasma membrane rupture during cell death. Glycine can be used to study cell protection, cancer, neurological diseases, and angiogenesis.

For research use only. We do not sell to patients.

CAS No. : 56-40-6

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10 mM * 1 mL in Water
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Based on 15 publication(s) in Google Scholar

Other Forms of Glycine:

Glycine Related Antibodies

Top Publications Citing Use of Products

    Glycine purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Mar 29;23(1):258.  [Abstract]

    (A) Schematic diagram of the synthesis steps of PN@Col nanoparticles(61 mg (-)-Epigallocatechin Gallate (EGCG), 10 mg Glycine and 2 mg Colivelin);(B) Morphological features of PN (top) and PN@Col (bottom) observed using TEM (scale bar: 100 nm/200 nm);(C) Morphology of PN (top) and PN@Col (bottom) observed using SEM (scale bar: 5 μm/1 μm);

    Glycine purchased from MedChemExpress. Usage Cited in: Diabetologia. 2024 Apr;67(4):738-754.  [Abstract]

    Representative image of kidneys stained with H&E, PAS and Masson (×400, scale bar, 25 μm) and TEM (×8000, scale bar, 1 μm) in different groups (Glycine (Gly，0.5-1% wt/vol.，drinking water for 12 weeks). The arrows indicate glomerular dilatation, mesangial matrix hyperplasia, and interstitial fibrosis, respectively.

    Glycine purchased from MedChemExpress. Usage Cited in: Diabetologia. 2024 Apr;67(4):738-754.  [Abstract]

    (a) Schematic of the animal experiment design. The experimental groups received water supplemented with Glycine (Gly，0.5-1% wt/vol., 12 weeks). (b) FBG levels in mice during the intervention. (c) Levels of insulin in the different groups. (d, e) Body weight (d) and food intake (e) in mice during the intervention.(f, g) Serum creatinine (f) and UACR (g) in the different groups.

    Glycine purchased from MedChemExpress. Usage Cited in: Diabetologia. 2024 Apr;67(4):738-754.  [Abstract]

    Western blot analysis of AMPK, p-AMPK, mTOR and p-mTOR protein expression in kidneys of experimental groups received water supplemented with Glycine (Gly，0.5-1% wt/vol., 12 weeks).

    Glycine purchased from MedChemExpress. Usage Cited in: Diabetologia. 2024 Apr;67(4):738-754.  [Abstract]

    Expression of AMPK, p-AMPK, mTOR, p-mTOR, BCL-2 and BAX in HK-2 cells with Glycine (GLY, 0.4-0.8 mM, 48 h) supplementation was detected by western blot.

    Glycine purchased from MedChemExpress. Usage Cited in: Diabetologia. 2024 Apr;67(4):738-754.  [Abstract]

    Cell viability of HK-2 cells in a high-glucose environment with Glycine (GLY, 0.4-0.8 mM, 48 h) supplementation was measured by CCK-8 assay.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Glycine is an inhibitory neurotransmitter in the CNS and also acts as a co-agonist along with glutamate, facilitating an excitatory potential at the glutaminergic N-methyl-D-aspartic acid (NMDA) receptors. Glycine is orally active. Glycine inhibits the membrane aggregation of NINJ1 and prevents plasma membrane rupture during cell death. Glycine can be used to study cell protection, cancer, neurological diseases, and angiogenesis[1][2][3][4][5][6].

    IC50 & Target[1][2][3][4][5][6]

    NMDA Receptor

     

    Human Endogenous Metabolite

     

    Microbial Metabolite

     

    NINJ1

     

    Cellular Effect
    Cell Line Type Value Description References
    HEK293 EC50
    106 μM
    Compound: 750
    Substrate uptake and inhibition of the Glycine Transporter-1 (GlyT1, SLC6A9) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC6A9 cells (PubChem AID: 1794809)
    Substrate uptake and inhibition of the Glycine Transporter-1 (GlyT1, SLC6A9) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC6A9 cells (PubChem AID: 1794809)
    		10.5281/zenodo.7361399
    HEK293 EC50
    128 μM
    Compound: 750
    Substrate uptake by the Glycine Transporter-2 (GlyT2, SLC6A5) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC6A5 cells (PubChem AID: 1794825)
    Substrate uptake by the Glycine Transporter-2 (GlyT2, SLC6A5) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC6A5 cells (PubChem AID: 1794825)
    		10.5281/zenodo.7360571
    HEK293 EC50
    94 μM
    Compound: glycine
    Antagonist activity at human glycine alpha-1 receptor in HEK293 cells by FMP assay
    Antagonist activity at human glycine alpha-1 receptor in HEK293 cells by FMP assay
    		[PMID: 17722904]
    KB 3-1 IC50
    > 50 mM
    Compound: glycine
    Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay
    Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay
    		[PMID: 21657271]
    KB-V1 IC50
    > 50 mM
    Compound: glycine
    Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay
    Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay
    		[PMID: 21657271]
    In Vitro

    Glycine (0-1 mM, 0-48 h) has anti-tumor and anti-angiogenic effects. It can downregulate the production of VEGF in tumor cells HCT-116, HT-29 and CC-531, inhibit the growth of endothelial cells HUVEC through GlyR, and indirectly inhibit HUVEC migration and capillary formation[3].
    Glycine (5 mM, 50 mM, 10 min) can prevent the aggregation of NINJ1 in the plasma membrane and inhibit cell death caused by the breakdown of NINJ1 dependent property membrane, thereby exerting its cytoprotective effect[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Glycine Related Antibodies

    Cell Migration Assay [3]

    Cell Line: HUVEC cells
    Concentration: 0, 0.01, 0.1, or 1 mM
    Incubation Time: 0-48 h
    Result: Reduced VEGF or conditioned medium-stimulated HUVEC migration and angiogenesis but had no direct effect on HUVEC migration.
    In Vivo

    Glycine (single 40-800 mg/kg, i.p.) dose-dependently prevents scopolamine (HY-N0296)-induced social cognitive impairment in adult rats[2].
    Glycine (500 and 1000 mg/kg/day for 14 days, i.p.) can significantly reduce kidney damage in lead-exposed mice by inhibiting ROS production[5].
    Glycine (single 1 or 2 g/kg, p.o.) increases extracellular serotonin but not dopamine in the prefrontal cortex of rats

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: renal injury in mice exposed to Pb, adult male BALB/c mice (25–30 g)[5]
    Dosage: 500 and 1000 mg/kg/day for 14 days
    Administration: i.p.
    Result: Significantly reduces elevated serum BUN and creatinine levels due to lead exposure.
    Significantly reduced ROS elevation in renal tissue due to lead exposure.
    Mitigated renal histopathological alterations in mice exposed to lead, included tubular dilation, protein cast, vacuolization, and inflammation.
    Clinical Trial
    Molecular Weight

    75.07

    Formula

    C2H5NO2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NCC(O)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Store at room temperature 3 years

    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    H2O : 25 mg/mL (333.02 mM; Need ultrasonic)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

    Methanol : < 1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 13.3209 mL 66.6045 mL 133.2090 mL
    5 mM 2.6642 mL 13.3209 mL 26.6418 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.93%

    SDS (393 KB)

    COA (243 KB) HNMR (215 KB) RP-HPLC (197 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 13.3209 mL 66.6045 mL 133.2090 mL 333.0225 mL
    5 mM 2.6642 mL 13.3209 mL 26.6418 mL 66.6045 mL
    10 mM 1.3321 mL 6.6605 mL 13.3209 mL 33.3023 mL
    15 mM 0.8881 mL 4.4403 mL 8.8806 mL 22.2015 mL
    20 mM 0.6660 mL 3.3302 mL 6.6605 mL 16.6511 mL
    25 mM 0.5328 mL 2.6642 mL 5.3284 mL 13.3209 mL
    30 mM 0.4440 mL 2.2202 mL 4.4403 mL 11.1008 mL
    40 mM 0.3330 mL 1.6651 mL 3.3302 mL 8.3256 mL
    50 mM 0.2664 mL 1.3321 mL 2.6642 mL 6.6605 mL
    60 mM 0.2220 mL 1.1101 mL 2.2202 mL 5.5504 mL
    80 mM 0.1665 mL 0.8326 mL 1.6651 mL 4.1628 mL
    100 mM 0.1332 mL 0.6660 mL 1.3321 mL 3.3302 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Glycine Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Glycine56-40-6VEGFRNinjurinEnvironmental PollutantsEndogenous MetaboliteiGluRVascular endothelial growth factor receptorEnvironmental ContaminantsIonotropic glutamate receptorscancerangiogenesisHCT-116 cellHT-29 cellCC-531 cellHUVEC cellcell migrationkidney damage in lead-exposed micesocial cognitive impairment in adult ratsInhibitorinhibitorinhibit

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