1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite

L-Alanine (Synonyms: L-2-Aminopropionic acid)

Cat. No.: HY-N0229 Purity: >98.0%
Handling Instructions

L-Alanine is a non-essential amino acid, involved in sugar and acid metabolism, increases immunity, and provides energy for muscle tissue, brain, and central nervous system.

For research use only. We do not sell to patients.

L-Alanine Chemical Structure

L-Alanine Chemical Structure

CAS No. : 56-41-7

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

L-Alanine is a non-essential amino acid, involved in sugar and acid metabolism, increases immunity, and provides energy for muscle tissue, brain, and central nervous system.

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

The viability of both hiPSCs, 201B7 cells and ehiPSCs decrease with an increase in L-Alanine concentration, and reach 7.5±1.3% and 3.7±0.7% respectively at 1.2 M of L-Alanine. On the other hand, no decrease in the viability of hFBs and hSkMCs are observed. Although the viability of iCMs slightly decreases along with the increase of the L-Alanine concentration, viability of iCMs at 1.2 M concentration of L-Alanine, 49.4±6.9%, is significantly higher than that of undifferentiated iPSCs, 201B7 cells and ehiPSCs (p< 0.01). The viability of hiPSCs, 201B7 cells and ehiPSCs, drastically decrease even after 2 or 4 h treatment. In contrast, the viability of hFBs fails to decrease at 1, 2, and 4 h and shows a small decrease at 24 h treatment. The viability of 201B7 cells in suspension culture decreases to 11.8±6.0% following treatment with 1.2 M L-Alanine for 2 h, whereas that of hFBs is 72.9±14.2%[1].

Clinical Trial
Solvent & Solubility
In Vitro: 

H2O : 150 mg/mL (1683.69 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 11.2246 mL 56.1230 mL 112.2460 mL
5 mM 2.2449 mL 11.2246 mL 22.4492 mL
10 mM 1.1225 mL 5.6123 mL 11.2246 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay

To investigate the selective removal of hiPSCs from differentiated cells by the high L-Alanine medium, two types of hiPSCs, 201B7 hiPSCs (201B7 cells) and an hiPSC line derived by episomal system (ehiPSCs) are used, along with normal human dermal fibroblasts (hFBs), human skeletal muscle cells (hSkMCs) and hiPSC-derived cardiomyocytes (iCMs) as differentiated cells. The cells are incubated in a medium supplemented with L-Alanine at various concentrations (0-1.2 M) or treatment times (1-24 h). The medium is replaced with a normal medium and the relative cell viability is measured after 24 h[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

89.09

Formula

C₃H₇NO₂

CAS No.

56-41-7

SMILES

N[[email protected]@H](C)C(O)=O

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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L-Alanine
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L-Alanine

Cat. No.: HY-N0229