SILAC Reagents
Stable isotope labeling by amino acids in cell culture (SILAC) is the most commonly used method for quantitative proteomic analysis, thanks to its simplicity and high reproducibility. It was first used in quantitative proteomics by Ong et al. from the Mann Laboratory in Denmark in 2022. It can provide accurate relative quantification without any chemical derivatization or other operations, and is widely used in research on cell biology, biochemistry, pharmacology, etc [1][2].
Workflow for SILAC experiments
Figure 1. Workflow for SILAC
References:
[1] Nat Protoc. 2006; 1(6):2650-60.
[2] Proteomics. 2015 Sep; 15(18):3175-92.
- Formula: C1513C6H2315NO4
- Molecular Weight: 360.36
Fmoc-Ile-OH-13C6,15N is the 13C and 15N labeled Fmoc-Ile-OH. Fmoc-Ile-OH is an isoleucine derivative.
- Formula: C24H2715NO6
- Molecular Weight: 426.47
Fmoc-Glu(OtBu)-OH-15N is a 15N-labeled Fmoc-Glu(OtBu)-OH.
- Formula: C1513C5H1915NO4
- Molecular Weight: 343.33
Fmoc-Pro-OH-13C5,15N is a 15N-labeled and 13C-labled Fmoc-Pro-OH (HY-W013780). Fmoc-Pro-OH is a proline derivative.
- Formula: C4H6D3NO2
- Molecular Weight: 106.14
(Rac)-2-Aminobutyric acid-d3 is the deuterium labeled (Rac)-2-Aminobutyric acid.
- Formula: 13C6H915N3O2
- Molecular Weight: 164.09
DL-Histidine-13C6,15N3 N-Acetyl-S-allyl-L-cysteine
- Formula: C1513C3H17NO4
- Molecular Weight: 314.31
Fmoc-Ala-OH-13C3 is a 13C-labeled Fmoc-Ala-OH (HY-W009204). Fmoc-Ala-OH is an alanine derivative.
- Formula: C1913C9H2915NO5
- Molecular Weight: 469.46
Fmoc-Tyr(tBu)-OH-13C9,15N is a 15N-labeled and 13C-labled Fmoc-Tyr(tBu)-OH (HY-W008016). Fmoc-Tyr(tBu)-OH is a tyrosine derivative.
- Formula: C1913C3H2515NO5
- Molecular Weight: 387.41
Fmoc-Ser(tBu)-OH-13C3,15N is a 15N-labeled and 13C-labled Fmoc-Ser(tBu)-OH (HY-W007941). Fmoc-Ser(tBu)-OH is a serine derivative.
- Formula: C1913C4H2715NO5
- Molecular Weight: 402.43
Fmoc-Thr(tBu)-OH-13C4,15N is a 15N-labeled and 13C-labled Fmoc-Thr(tBu)-OH (HY-W007706). Fmoc-Thr(tBu)-OH is a threonine derivative.
- Formula: C1513C5H2115NO4
- Molecular Weight: 345.34
Fmoc-L-Val-OH-13C5,15N is a 15N-labeled and 13C-labled Fmoc-L-Val-OH (HY-I1111). Fmoc-L-Val-OH is a valine derivative.
- Formula: C19H16D3NO4
- Molecular Weight: 328.38
Fmoc-Aib-OH-d3 is the deuterium labeled Fmoc-Aib-OH (HY-79648). Fmoc-Aib-OH is an alanine derivative.
- Formula: C313CH10ClNO2
- Molecular Weight: 140.57
- Formula: C213C2H10ClNO2
- Molecular Weight: 141.57
- Formula: C6H8D8Cl2N2O2
- Molecular Weight: 227.16
DL-Lysine-d8 (dihydrochloride) is the deuterium labeled DL-Lysine.
- Formula: C5H9D2NO2
- Molecular Weight: 119.16
DL-Valine-d2 is the deuterium labeled DL-Valine.
- Formula: C5H10DNO2
- Molecular Weight: 118.15
DL-Valine-d-1 is the deuterium labeled DL-Valine.
- Formula: C5H10DNO2
- Molecular Weight: 118.15
DL-Valine-d is the deuterium labeled DL-Valine.
- Formula: C3H5D3ClNO2
- Molecular Weight: 128.57
2-(Methylamino)acetic acid-d3 (hydrochloride) is the deuterium labeled 2-(Methylamino)acetic acid.
- Formula: C18H13D4NO4
- Molecular Weight: 315.36
Fmoc-Ala-OH-d4 is the deuterium labeled Fmoc-Ala-OH.
- Formula: C9H8D3NO3
- Molecular Weight: 184.21
DL-m-Tyrosine-d3 is the deuterium labeled DL-m-Tyrosine (HY-W001940). DL-m-Tyrosine is a non-protein amino acid. DL-m-Tyrosine inhibits microbial growth and spore formation. DL-m-Tyrosine also inhibits root growth in various plants, such as lettuce and Arabidopsis. DL-m-Tyrosine, when used in combination with Carbidopa (HY-B0311), has a potent antihypertensive effect. DL-m-Tyrosine can be used in research on biopesticide design.
