N-Carbamoyl-L-aspartic acid
Based on 1 publication(s) in Google Scholar
N-Carbamoyl-L-aspartic acid (L-Ureidosuccinic acid) is an important pyrimidine metabolic precursor and intermediate metabolite. N-Carbamoyl-L-aspartic acid reverses the growth inhibition of Ura+ strains induced by 2-thiouracil (with growth rate increasing linearly with its concentration), but fails to support the growth of uracil-requiring Ura- strains. N-Carbamoyl-L-aspartic acid inhibits the cell growth of *Saccharomyces cerevisiae* by suppressing the purine biosynthetic pathway at a pre-step of 5-aminoimidazole nucleotide synthesis. The growth inhibitory effect of N-Carbamoyl-L-aspartic acid on yeast can be alleviated by purines, and the sensitivity of strains is closely related to the activity level of dihydroorotase.
For research use only. We do not sell to patients.
- Purity: 99.83%
- CAS No.: 13184-27-5
- Formula: C5H8N2O5
- Molecular Weight:176.13
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) N-Carbamoyl-L-aspartic acid
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Biological Activity
N-Carbamoyl-L-aspartic acid (25-100 μg/mL, 10 mg/mL; up to 70 hrs) potently inhibits growth of Saccharomyces cerevisiae wild type FL 100, ura2-60, ura4-20, and ure1 strains, while ura1-8, ura3-6, and ura5-5 strains are highly resistant to this inhibition[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Saccharomyces cerevisiae strains (wild type FL 100, ura2-60, ura4-20, ure1, ura1-8, ura3-6, ura5-5)
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Concentration:25 μg/mL, 50 μg/mL, 100 μg/mL, 10 mg/mL; 100 μg/mL
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Incubation Time:up to 70 hrs; 375 min
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Result:Dramatically inhibited growth of wild type FL 100, ura2-60, ura4-20, and ure1 strains, with initial reduced growth rates that returned to normal after 12-20 hrs (depending on concentration).
Matched untreated cell density by 48 hrs with 100 μg/mL treatment.
Showed no concentration-dependent recovery of normal growth rate in ura4-20 strain.
Only slightly or not inhibited ura1-8, ura3-6, and ura5-5 strains.
Created a halo of no growth surrounding ura4-20 streak near ura2-60 lawns, confirming it is the inhibitory enantiomorph.
Showed unimpeded growth of USA-resistant ura2-60 derivative adjacent to ura4-20.
Chemical Information
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CAS No. 13184-27-5
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Appearance Solid
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Molecular Weight 176.13
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Formula C5H8N2O5
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Color White to off-white
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SMILES
O=C(O)C[C@@H](C(O)=O)NC(N)=O
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Synonyms
L-Ureidosuccinic acid
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Mol Cell
2025 Dec 4;85(23):4347-4364.e12. PMID: 41270758
Solvent & Solubility
H2O : 31.25 mg/mL (177.43 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 5.6776 mL | 28.3881 mL | 56.7762 mL | 141.9406 mL |
| 5 mM | 1.1355 mL | 5.6776 mL | 11.3552 mL | 28.3881 mL | |
| 10 mM | 0.5678 mL | 2.8388 mL | 5.6776 mL | 14.1941 mL | |
| 15 mM | 0.3785 mL | 1.8925 mL | 3.7851 mL | 9.4627 mL | |
| 20 mM | 0.2839 mL | 1.4194 mL | 2.8388 mL | 7.0970 mL | |
| 25 mM | 0.2271 mL | 1.1355 mL | 2.2710 mL | 5.6776 mL | |
| 30 mM | 0.1893 mL | 0.9463 mL | 1.8925 mL | 4.7314 mL | |
| 40 mM | 0.1419 mL | 0.7097 mL | 1.4194 mL | 3.5485 mL | |
| 50 mM | 0.1136 mL | 0.5678 mL | 1.1355 mL | 2.8388 mL | |
| 60 mM | 0.0946 mL | 0.4731 mL | 0.9463 mL | 2.3657 mL | |
| 80 mM | 0.0710 mL | 0.3549 mL | 0.7097 mL | 1.7743 mL | |
| 100 mM | 0.0568 mL | 0.2839 mL | 0.5678 mL | 1.4194 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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