Nimotuzumab  (Synonyms: h-R3)

Nimotuzumab is a humanized IgG1 monoclonal antibody targeting EGFR with a K_D of 0.21 nM. Nimotuzumab is directed against the extracellular domain of the EGFR blocking the binding to its ligands. Nimotuzumab, a strong antitumor agent, is cytolytic on target tumors by its capacity to cause antibody dependent cell mediated cytotoxicity (ADCC) and complement dependent cytotoxicity (CDC)^[1][2].

Human IgG1 kappa

Human IgG1 kappa, Isotype Control

Human

ERBB1/EGFR/HER1

Nimotuzumab (10 μg/mL; 24 hours) induces significant downregulation of CD16 on NK cells^[1].
Nimotuzumab (10 μg/mL; 48 hours) induces the upregulation of PD-L1 molecule on DCs when co-cultured with the NK: DC: HNSCC cells^[1].
The intrinsic properties of Nimotuzumab requires bivalent binding (i.e., binding with both antibody arms to two targets simultaneously) for stable attachment to cellular surface, which leads to Nimotuzumab selectively binding to cells that express moderate to high EGFR levels^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1956  [NCBI]

P00533

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

147.58 kDa

780758-10-3

Liquid

Colorless to light yellow

[Nimotuzumab]

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

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  Human IgG1 kappa

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  Immobilized EGFR vIII Protein, Human (HEK293, His, HY-P70189) can bind Nimotuzumab. The EC₅₀ for this effect is 19 ng/mL.
• 
  Flow Cytometry analysis of A431 cells labelling ERBB1/EGFR/HER1 (red) with Nimotuzumab (anti-ERBB1/EGFR/HER1) (HY-P9968). Goat Anti-Human IgG (Alexa Fluor 488) (HY-P83776) at a dilution of 1/1000 was used as the secondary antibody. Blue-Human IgG1 kappa (HY-P99001). Black-Unlabelled control, cells without incubation with primary antibody.

• [1]. Zaima Mazorra, et al. Nimotuzumab Induces NK Cell Activation, Cytotoxicity, Dendritic Cell Maturation and Expansion of EGFR-Specific T Cells in Head and Neck Cancer Patients. Front Pharmacol. 2017 Jun 19;8:382.  [Content Brief]

  [2]. Melarkode S Ramakrishnan, et al. Nimotuzumab, a promising therapeutic monoclonal for treatment of tumors of epithelial origin. MAbs. Jan-Feb 2009;1(1):41-8.  [Content Brief]