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  3. OVA Peptide(257-264) acetate salt

OVA Peptide(257-264) acetate salt is a class I (Kb)-restricted peptide epitope of OVA, an octameric peptide can be from ovalbumin presented by the class I MHC molecule, H-2Kb.

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Top Publications Citing Use of Products

    OVA Peptide(257-264) acetate salt purchased from MedChemExpress. Usage Cited in: BIO Web of Conferences. 2025 Jul 2.

    OT-1 CD8+ T cells were stimulated with OVA Peptide(257-264) TFA (SIINFEKL peptide) (1 ug/mL; 24-48 h) and sampled for CFSE detection via flow cytometry.

    OVA Peptide(257-264) acetate salt purchased from MedChemExpress. Usage Cited in: BIO Web of Conferences. 2025 Jul 2.

    B16F10-FLuc cells were pre-treated with/without OVA Peptide(257-264) TFA (SIINFEKL peptide) (25 nM; 2 h) and then co-cultured with OT-1 CD8+ T cells for 4 h. Luminescence was measured by mixing 50 μL of supernatant with 100 μL of luciferase substrate.

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    • Biological Activity

    • 순도&문서

    • References

    • 고객리뷰

    제품 설명

    OVA Peptide(257-264) acetate salt is a class I (Kb)-restricted peptide epitope of OVA, an octameric peptide can be from ovalbumin presented by the class I MHC molecule, H-2Kb[1].

    In Vitro

    TAP1-I- and C57BL/6 macrophages may process Crl-OVA and full-length OVA in different cellular compartments and that the protein context of the OVA Peptide(257-264) epitope influences the extent of TAP-independent processing for MHC class I presentation. OVA Peptide(257-264) epitope is presented with a differential dependence on the TAP transporter depending on the protein context of the OVA epitope: OVA Peptide(257-264) contained within the MBPCrl-OVA or Crl-OVA bacterial fusion proteins is presented with little dependence on the TAP transporter, while OVA Peptide(257-264) contained within full-length ovalbumin is largely dependent on the TAP transporter, regardless of whether recombinant OVA is expressed in bacteria or the native protein is coupled to polystyrene beads[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    분자량

    1023.18

    화학식

    C47H78N10O15

    선적

    Room temperature in continental US; may vary elsewhere.

    보관

    Please store the product under the recommended conditions in the Certificate of Analysis.

    용액&용해도
    In Vitro: 

    H2O

    Peptide Solubility and Storage Guidelines:

    1.  Calculate the length of the peptide.

    2.  Calculate the overall charge of the entire peptide according to the following table:

      Contents Assign value
    Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
    Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
    Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

    3.  Recommended solution:

    Overall charge of peptide Details
    Negative (<0) 1.  Try to dissolve the peptide in water first.
    2.  If water fails, add NH4OH (<50 μL).
    3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
    Positive (>0) 1.  Try to dissolve the peptide in water first.
    2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
    3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
    Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
    2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
    순도&문서
    References
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    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2
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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    상품명:
    OVA Peptide(257-264) acetate salt
    Cat. No.:
    HY-P1489B
    수량:
    MCE Japan Authorized Agent: