2. Immunology/Inflammation
  3. MHC
  4. OVA Peptide(257-264)

OVA Peptide(257-264) is a class I (Kb)-restricted peptide epitope of OVA, an octameric peptide can be from ovalbumin presented by the class I MHC molecule, H-2Kb.

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CAS No. : 138831-86-4

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Based on 15 publication(s) in Google Scholar

Other Forms of OVA Peptide(257-264):

OVA Peptide(257-264) Related Antibodies

Top Publications Citing Use of Products

    OVA Peptide(257-264) purchased from MedChemExpress. Usage Cited in: BIO Web of Conferences. 2025 Jul 2.

    OT-1 CD8+ T cells were stimulated with OVA Peptide(257-264) TFA (SIINFEKL peptide) (1 ug/mL; 24-48 h) and sampled for CFSE detection via flow cytometry.

    OVA Peptide(257-264) purchased from MedChemExpress. Usage Cited in: BIO Web of Conferences. 2025 Jul 2.

    B16F10-FLuc cells were pre-treated with/without OVA Peptide(257-264) TFA (SIINFEKL peptide) (25 nM; 2 h) and then co-cultured with OT-1 CD8+ T cells for 4 h. Luminescence was measured by mixing 50 μL of supernatant with 100 μL of luciferase substrate.

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    MHC I MHC II

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    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    OVA Peptide(257-264) is a class I (Kb)-restricted peptide epitope of OVA, an octameric peptide can be from ovalbumin presented by the class I MHC molecule, H-2Kb.

    In Vitro

    TAP1-I- and C57BL/6 macrophages may process Crl-OVA and full-length OVA in different cellular compartments and that the protein context of the OVA Peptide(257-264) epitope influences the extent of TAP-independent processing for MHC class I presentation. OVA Peptide(257-264) epitope is presented with a differential dependence on the TAP transporter depending on the protein context of the OVA epitope: OVA Peptide(257-264) contained within the MBPCrl-OVA or Crl-OVA bacterial fusion proteins is presented with little dependence on the TAP transporter, while OVA Peptide(257-264) contained within full-length ovalbumin is largely dependent on the TAP transporter, regardless of whether recombinant OVA is expressed in bacteria or the native protein is coupled to polystyrene beads[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    OVA Peptide(257-264) Related Antibodies
    Molecular Weight

    963.13

    Formula

    C45H74N10O13
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu
    Sequence Shortening

    SIINFEKL
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 40 mg/mL (41.53 mM; ultrasonic and adjust pH to 3 with 1 M HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    0.1% TFA in Water : 1.83 mg/mL (1.90 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.0383 mL 5.1914 mL 10.3828 mL
    5 mM 0.2077 mL 1.0383 mL 2.0766 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
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    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.65%

    SDS (252 KB)

    COA (248 KB) RP-HPLC (251 KB) MS (69 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay

    TAPl-/- or C57BL/6 macrophages are co-incubated with either bacteria or polystyrene beads containing the 257-264 epitope from ovalbumin [OVA Peptide(257-264)], which binds the mouse class I molecule Kb. The source of the OVA(257-264) epitope is either the Crl-OVA(257-264) (Crl-OVA) fusion protein, the maltose binding protein (MBP)-Crl-OVA fusion protein, native OVA or bacterial recombinant OVA (rOVA); Crl-OVA, MBP-Crl-OVA and rOVA are each expressed in bacteria, and Crl-OVA and MBP-Crl-OVA purified from bacterial lysates and native egg OVA are coated onto polystyrene beads[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    0.1% TFA in Water / DMSO 1 mM 1.0383 mL 5.1914 mL 10.3828 mL 25.9570 mL
    DMSO 5 mM 0.2077 mL 1.0383 mL 2.0766 mL 5.1914 mL
    10 mM 0.1038 mL 0.5191 mL 1.0383 mL 2.5957 mL
    15 mM 0.0692 mL 0.3461 mL 0.6922 mL 1.7305 mL
    20 mM 0.0519 mL 0.2596 mL 0.5191 mL 1.2979 mL
    25 mM 0.0415 mL 0.2077 mL 0.4153 mL 1.0383 mL
    30 mM 0.0346 mL 0.1730 mL 0.3461 mL 0.8652 mL
    40 mM 0.0260 mL 0.1298 mL 0.2596 mL 0.6489 mL
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    OVA Peptide(257-264) Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    OVA Peptide(257-264)138831-86-4MHCMajor Histocompatibility ComplexInhibitorinhibitorinhibit

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