OVA Peptide(257-264) TFA

Name: OVA Peptide(257-264) TFA
Brand: MedChemExpress
SKU: HY-P1489A
Rating: 5.0 (15 reviews)

Cat. No.: HY-P1489A Purity: 99.91%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

OVA Peptide(257-264) TFA is a class I (Kb)-restricted peptide epitope of OVA, an octameric peptide can be from ovalbumin presented by the class I MHC molecule, H-2Kb.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

CAS No. : 1262751-08-5

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 15 publication(s) in Google Scholar

Other Forms of OVA Peptide(257-264) TFA:

OVA Peptide(257-264) In-stock
OVA Peptide(257-264) acetate salt Get quote

15 Publications Citing Use of MCE OVA Peptide(257-264) TFA

Flow Cytometry

Cell Imaging/Staining

: OVA Peptide(257-264) TFA purchased from MedChemExpress. Usage Cited in: BIO Web of Conferences. 2025 Jul 2.; OT-1 CD8⁺ T cells were stimulated with OVA Peptide(257-264) TFA (SIINFEKL peptide) (1 ug/mL; 24-48 h) and sampled for CFSE detection via flow cytometry.

: OVA Peptide(257-264) TFA purchased from MedChemExpress. Usage Cited in: BIO Web of Conferences. 2025 Jul 2.; B16F10-FLuc cells were pre-treated with/without OVA Peptide(257-264) TFA (SIINFEKL peptide) (25 nM; 2 h) and then co-cultured with OT-1 CD8⁺ T cells for 4 h. Luminescence was measured by mixing 50 μL of supernatant with 100 μL of luciferase substrate.

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

OVA Peptide(257-264) TFA is a class I (Kb)-restricted peptide epitope of OVA, an octameric peptide can be from ovalbumin presented by the class I MHC molecule, H-2Kb.

In Vitro

TAP1-I- and C57BL/6 macrophages may process Crl-OVA and full-length OVA in different cellular compartments and that the protein context of the OVA Peptide(257-264) epitope influences the extent of TAP-independent processing for MHC class I presentation. OVA Peptide(257-264) epitope is presented with a differential dependence on the TAP transporter depending on the protein context of the OVA epitope: OVA Peptide(257-264) contained within the MBPCrl-OVA or Crl-OVA bacterial fusion proteins is presented with little dependence on the TAP transporter, while OVA Peptide(257-264) contained within full-length ovalbumin is largely dependent on the TAP transporter, regardless of whether recombinant OVA is expressed in bacteria or the native protein is coupled to polystyrene beads^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1077.15

Formula

C₄₇H₇₅F₃N₁₀O₁₅

CAS No.

1262751-08-5

Appearance

Solid

Color

White to off-white

Sequence

Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu

Sequence Shortening

SIINFEKL

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (116.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 5 mg/mL (4.64 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.9284 mL	4.6419 mL	9.2838 mL
5 mM	0.1857 mL	0.9284 mL	1.8568 mL
10 mM	0.0928 mL	0.4642 mL	0.9284 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (1.93 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (250 KB) RP-HPLC (250 KB) MS (72 KB)

Handling Instructions (2659 KB)

References

[1]. Wick MJ, et al. Major histocompatibility complex class I presentation of ovalbumin peptide 257-264 from exogenous sources: protein context influences the degree of TAP-independent presentation. Eur J Immunol. 1996 Nov;26(11):2790-9. [Content Brief]

Cell Assay

TAPl^-/- or C57BL/6 macrophages are co-incubated with either bacteria or polystyrene beads containing the 257-264 epitope from ovalbumin [OVA Peptide(257-264)], which binds the mouse class I molecule Kb. The source of the OVA(257-264) epitope is either the Crl-OVA(257-264) (Crl-OVA) fusion protein, the maltose binding protein (MBP)-Crl-OVA fusion protein, native OVA or bacterial recombinant OVA (rOVA); Crl-OVA, MBP-Crl-OVA and rOVA are each expressed in bacteria, and Crl-OVA and MBP-Crl-OVA purified from bacterial lysates and native egg OVA are coated onto polystyrene beads^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Wick MJ, et al. Major histocompatibility complex class I presentation of ovalbumin peptide 257-264 from exogenous sources: protein context influences the degree of TAP-independent presentation. Eur J Immunol. 1996 Nov;26(11):2790-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	0.9284 mL	4.6419 mL	9.2838 mL	23.2094 mL
DMSO	5 mM	0.1857 mL	0.9284 mL	1.8568 mL	4.6419 mL
	10 mM	0.0928 mL	0.4642 mL	0.9284 mL	2.3209 mL
	15 mM	0.0619 mL	0.3095 mL	0.6189 mL	1.5473 mL
	20 mM	0.0464 mL	0.2321 mL	0.4642 mL	1.1605 mL
	25 mM	0.0371 mL	0.1857 mL	0.3714 mL	0.9284 mL
	30 mM	0.0309 mL	0.1547 mL	0.3095 mL	0.7736 mL
	40 mM	0.0232 mL	0.1160 mL	0.2321 mL	0.5802 mL
	50 mM	0.0186 mL	0.0928 mL	0.1857 mL	0.4642 mL
	60 mM	0.0155 mL	0.0774 mL	0.1547 mL	0.3868 mL
	80 mM	0.0116 mL	0.0580 mL	0.1160 mL	0.2901 mL
	100 mM	0.0093 mL	0.0464 mL	0.0928 mL	0.2321 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.