Phenylthiourea (Synonyms: Phenylthiocarbamide)

Name: Phenylthiourea
Brand: MedChemExpress
SKU: HY-Y0351
Rating: 5.0 (3 reviews)

Cat. No.: HY-Y0351 Pureza: 99.26%: Ficha de datos
COA

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Phenylthiourea (Phenylthiocarbamide) is an inhibitor for phenoloxidase. Phenylthiourea inhibits enzymatic oxidation of DOPA by phenoloxidase (K_i = 0.21 μM). Phenylthiourea is an effective inhibitor for tyrosinase. Phenylthiourea can lead to graying of hair in black rats due to the interference with melanin formation.

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Phenylthiourea Estructura química

No. CAS : 103-85-5

Tamaño	Stock	Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
5 g	En stock	Estimated Time of Arrival: December 31
10 g	En stock	Estimated Time of Arrival: December 31
25 g	En stock	Estimated Time of Arrival: December 31
50 g	En stock	Estimated Time of Arrival: December 31
100 g	En stock	Estimated Time of Arrival: December 31
> 100 g	Obtener un presupuesto

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Revisión del cliente

Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE Phenylthiourea

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Descripciòn

IC₅₀ & Target

phenoloxidase^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
B16	IC₅₀	1.8 μM Compound: PTU	Inhibition of tyrosinase in alpha-MSH-induced mouse B16 cells Inhibition of tyrosinase in alpha-MSH-induced mouse B16 cells	[PMID: 20638280]
B16	IC₅₀	5.4 μM Compound: 1a	Inhibition of melanogenesis in mouse B16 cells Inhibition of melanogenesis in mouse B16 cells	[PMID: 20359890]

In Vitro

Phenylthiourea (Compound PTU) (0-20 μM, 0-300 s) affects the melanization process by either inhibiting the action of phenoloxidase directly or reducing DOPA-quinine to DOPA^[1].
Phenylthiourea (0-2.5 μM) has inhibitory effect on DOPA oxidation catalyzed by phenoloxidase (K_i = 0.21 μM)^[1].
Phenylthiourea (Compound Phenylthiocarbamide) inhibits the methemoglobin production in rat tissues^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

152.22

Fòrmula

C₇H₈N₂S

No. CAS

103-85-5

Appearance

Solid

Color

White to off-white

SMILES

S=C(NC1=CC=CC=C1)N

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (656.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.5694 mL	32.8472 mL	65.6944 mL
5 mM	1.3139 mL	6.5694 mL	13.1389 mL
10 mM	0.6569 mL	3.2847 mL	6.5694 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (16.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (16.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (16.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 99.26%

Select Batch:

Ficha de datos (273 KB) SDS (480 KB)

COA (245 KB) HNMR (233 KB) RP-HPLC (247 KB) LCMS (339 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Ryazanova AD, et al. The phenylthiourea is a competitive inhibitor of the enzymatic oxidation of DOPA by phenoloxidase. J Enzyme Inhib Med Chem. 2012 Feb;27(1):78-83. [Content Brief]

[2]. Frederick Bernheim & Mary L.C. Bernheim (1942) THE ACTION OF PHENYLTHIOCARBAMIDE ON TYROSINASE, Journal of Biological Chemistry, Volume 145, Issue 1, Pages 213-217, ISSN 0021-9258.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.5694 mL	32.8472 mL	65.6944 mL	164.2360 mL
	5 mM	1.3139 mL	6.5694 mL	13.1389 mL	32.8472 mL
	10 mM	0.6569 mL	3.2847 mL	6.5694 mL	16.4236 mL
	15 mM	0.4380 mL	2.1898 mL	4.3796 mL	10.9491 mL
	20 mM	0.3285 mL	1.6424 mL	3.2847 mL	8.2118 mL
	25 mM	0.2628 mL	1.3139 mL	2.6278 mL	6.5694 mL
	30 mM	0.2190 mL	1.0949 mL	2.1898 mL	5.4745 mL
	40 mM	0.1642 mL	0.8212 mL	1.6424 mL	4.1059 mL
	50 mM	0.1314 mL	0.6569 mL	1.3139 mL	3.2847 mL
	60 mM	0.1095 mL	0.5475 mL	1.0949 mL	2.7373 mL
	80 mM	0.0821 mL	0.4106 mL	0.8212 mL	2.0529 mL
	100 mM	0.0657 mL	0.3285 mL	0.6569 mL	1.6424 mL

Phenylthiourea Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phenylthiourea103-85-5PhenylthiocarbamideTyrosinaseEndogenous MetaboliteDOPAphenoloxidaseInhibitorinhibitorinhibit

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Phenylthiourea (Synonyms: Phenylthiocarbamide)

Revisión del cliente

Phenylthiourea Related Antibodies

3 Publications Citing Use of MCE Phenylthiourea

Recomendaciones destacadas

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas Endogenous Metabolite:

Actividad biológica

Pureza y Documentación

Referencias

Revisión del cliente

Phenylthiourea Related Antibodies

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

Phenylthiourea Related Classifications

Keywords:

Productos vistos recientemente:

Revisión del cliente

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SAFETY DATA SHEET (SDS)

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