2. Cell Cycle/DNA Damage Metabolic Enzyme/Protease
  3. DNA Alkylator/Crosslinker Drug Metabolite
  4. Phosphoramide mustard

Phosphoramide mustard is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard induces DNA damage.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (Phosphoramide mustard cyclohexanamine) that retains the same biological activity.

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CAS 番号 : 10159-53-2

容量 在庫状況
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Other 在庫あり Forms of Phosphoramide mustard:

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Phosphoramide mustard 関連抗体

Top Publications Citing Use of Products

    Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

    Phosphoramide mustard cyclohexanamine (PM) (3–120 μM; 48 h) significantly inhibited Caco-2 cell viability compared with the control-treated group.

    Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

    Phosphoramide mustard cyclohexanamine (PM) (15–120 μM; 48 h) (PM) promoted the increase of MDA content in Caco-2 cells in a concentration-dependent manner.

    Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

    Phosphoramide mustard cyclohexanamine (PM) (15–120 μM; 48 h) significantly enhanced the red fluorescence intensity emitted by RhoNox-1 staining, indicating the specific accumulation of Fe2+ in Caco-2 cells.

    Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

    Phosphoramide mustard cyclohexanamine (PM) (15–120 μM; 48 h) treatment of Caco-2 cells caused overexpression of NCOA4 protein in a concentration-dependent manner.

    Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

    Phosphoramide mustard cyclohexanamine (PM) (30 μM; 48 h) induced autophagy in Caco-2 cells, as evidenced by increased autophagosomes and autophagolysosomes.

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    製品説明

    Phosphoramide mustard is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard induces DNA damage[1][2].

    IC50 & Target

    DNA Alkylator[1]
    Cellular Effect
    Cell Line Type Value Description References
    V79 IC50
    77 3
    Compound: 27
    In vitro cytotoxicity against V-79 chinese hamster lung fibroblasts (3 hr drug exposure time) by clonogenic assay
    In vitro cytotoxicity against V-79 chinese hamster lung fibroblasts (3 hr drug exposure time) by clonogenic assay
    		[PMID: 15239662]
    V79 IC50
    77 3
    Compound: 27
    In vitro cytotoxicity against V-79 chinese hamster lung fibroblasts (3 hr drug exposure time) by clonogenic assay
    In vitro cytotoxicity against V-79 chinese hamster lung fibroblasts (3 hr drug exposure time) by clonogenic assay
    		[PMID: 15239662]
    V79 IC50
    77 3
    Compound: 27
    In vitro cytotoxicity against V-79 chinese hamster lung fibroblasts (3 hr drug exposure time) by clonogenic assay
    In vitro cytotoxicity against V-79 chinese hamster lung fibroblasts (3 hr drug exposure time) by clonogenic assay
    		[PMID: 15239662]
    体外実験

    Phosphoramide mustard causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication[1].
    Phosphoramide mustard (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells (SIGCs)[1].
    Phosphoramide mustard (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage[1].
    Phosphoramide mustard (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Phosphoramide mustard 関連抗体

    Cell Viability Assay[1]

    Cell Line: SIGCs
    Concentration: 0.5 μM, 1 μM, 3 μM, 6 μM
    Incubation Time: 48 hours
    Result: Reduced cell viability at concentrations of 3 μM and higher.

    RT-PCR[1]

    Cell Line: SIGCs
    Concentration: 3 μM, 6 μM
    Incubation Time: 24 hours, 48 hours
    Result: Increased DDR gene mRNA expression levels.

    Western Blot Analysis[1]

    Cell Line: SIGCs
    Concentration: 3 μM, 6 μM
    Incubation Time: 24 hours, 48 hours
    Result: Generally increased DDR proteins.
    体内実験

    Phosphoramide mustard (2.1-20.7 mg/kg; i.p.; daily; for 5 days) inhibits subcutaneous tumor growth in rats[2].
    Phosphoramide mustard exhibits terminal elimination half-lives (rat 15.1 min) following intravenous administration (rat 59.4 mg/kg)[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Rat, subcutaneously implanted Walker 256 carcinosarcoma tumor[2]
    Dosage: 2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg
    Administration: Intraperitoneal injection, once daily, for 5 consecutive days
    Result: Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg.
    Animal Model: Rats[2]
    Dosage: 59.4 mg/kg (Pharmacokinetic Analysis)
    Administration: Intravenous injection
    Result: T1/2 (15.1 min).
    分子量

    221.02

    分子式

    C4H11Cl2N2O2P
    CAS 番号
    SMILES

    O=P(N(CCCl)CCCl)(N)O
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    Please store the product under the recommended conditions in the Certificate of Analysis.
    純度とドキュメンテーション
    参考文献
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    Phosphoramide mustard Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Phosphoramide mustard10159-53-2DNA Alkylator/CrosslinkerDrug Metabolitecyclophosphamidephosphoramide mustardDNAadductdamagerepairgenotoxicityalkylatorInhibitorinhibitorinhibit

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    製品名:
    Phosphoramide mustard
    製品番号:
    HY-137316
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