2. Cell Cycle/DNA Damage Metabolic Enzyme/Protease
  3. DNA Alkylator/Crosslinker Drug Metabolite
  4. Phosphoramide mustard cyclohexanamine

Phosphoramide mustard cyclohexanamine 

Cat. No.: HY-137316A Purity: 98.0%
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Phosphoramide mustard cyclohexanamine is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard cyclohexanamine induces DNA damage.

For research use only. We do not sell to patients.

CAS No. : 1566-15-0

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of Phosphoramide mustard cyclohexanamine:

Phosphoramide mustard cyclohexanamine Related Antibodies

Top Publications Citing Use of Products

    Phosphoramide mustard cyclohexanamine purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

    Phosphoramide mustard cyclohexanamine (PM) (3–120 μM; 48 h) significantly inhibited Caco-2 cell viability compared with the control-treated group.

    Phosphoramide mustard cyclohexanamine purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

    Phosphoramide mustard cyclohexanamine (PM) (15–120 μM; 48 h) (PM) promoted the increase of MDA content in Caco-2 cells in a concentration-dependent manner.

    Phosphoramide mustard cyclohexanamine purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

    Phosphoramide mustard cyclohexanamine (PM) (15–120 μM; 48 h) significantly enhanced the red fluorescence intensity emitted by RhoNox-1 staining, indicating the specific accumulation of Fe2+ in Caco-2 cells.

    Phosphoramide mustard cyclohexanamine purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

    Phosphoramide mustard cyclohexanamine (PM) (15–120 μM; 48 h) treatment of Caco-2 cells caused overexpression of NCOA4 protein in a concentration-dependent manner.

    Phosphoramide mustard cyclohexanamine purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

    Phosphoramide mustard cyclohexanamine (PM) (30 μM; 48 h) induced autophagy in Caco-2 cells, as evidenced by increased autophagosomes and autophagolysosomes.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Phosphoramide mustard cyclohexanamine is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard cyclohexanamine induces DNA damage[1][2].

    In Vitro

    Phosphoramide mustard cyclohexanamine causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication[1].
    Phosphoramide mustard cyclohexanamine (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells (SIGCs)[1].
    Phosphoramide mustard cyclohexanamine (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage[1].
    Phosphoramide mustard cyclohexanamine (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Phosphoramide mustard cyclohexanamine Related Antibodies

    Cell Viability Assay[1]

    Cell Line: SIGCs
    Concentration: 0.5 μM, 1 μM, 3 μM, 6 μM
    Incubation Time: 48 hours
    Result: Reduced cell viability at concentrations of 3 μM and higher.

    RT-PCR[1]

    Cell Line: SIGCs
    Concentration: 3 μM, 6 μM
    Incubation Time: 24 hours, 48 hours
    Result: Increased DDR gene mRNA expression levels.

    Western Blot Analysis[1]

    Cell Line: SIGCs
    Concentration: 3 μM, 6 μM
    Incubation Time: 24 hours, 48 hours
    Result: Generally increased DDR proteins.
    In Vivo

    Phosphoramide mustard cyclohexanamine (2.1-20.7 mg/kg; i.p.; daily; for 5 days) inhibits subcutaneous tumor growth in rats[2].
    Phosphoramide mustard cyclohexanamine exhibits terminal elimination half-lives (rat 15.1 min) following intravenous administration (rat 59.4 mg/kg)[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Rat, subcutaneously implanted Walker 256 carcinosarcoma tumor[2]
    Dosage: 2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg
    Administration: Intraperitoneal injection, once daily, for 5 consecutive days
    Result: Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg.
    Animal Model: Rats[2]
    Dosage: 86.0 mg/kg (Pharmacokinetic Analysis)
    Administration: Intravenous injection
    Result: T1/2 (15.1 min).
    Molecular Weight

    320.20

    Formula

    C10H24Cl2N3O2P
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=P(N(CCCl)CCCl)(N)O.NC1CCCCC1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, protect from light, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 50 mg/mL (156.15 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1230 mL 15.6152 mL 31.2305 mL
    5 mM 0.6246 mL 3.1230 mL 6.2461 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (312.30 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 98.0%

    SDS (393 KB)

    COA (253 KB) HNMR (362 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 3.1230 mL 15.6152 mL 31.2305 mL 78.0762 mL
    5 mM 0.6246 mL 3.1230 mL 6.2461 mL 15.6152 mL
    10 mM 0.3123 mL 1.5615 mL 3.1230 mL 7.8076 mL
    15 mM 0.2082 mL 1.0410 mL 2.0820 mL 5.2051 mL
    20 mM 0.1562 mL 0.7808 mL 1.5615 mL 3.9038 mL
    25 mM 0.1249 mL 0.6246 mL 1.2492 mL 3.1230 mL
    30 mM 0.1041 mL 0.5205 mL 1.0410 mL 2.6025 mL
    40 mM 0.0781 mL 0.3904 mL 0.7808 mL 1.9519 mL
    50 mM 0.0625 mL 0.3123 mL 0.6246 mL 1.5615 mL
    60 mM 0.0521 mL 0.2603 mL 0.5205 mL 1.3013 mL
    80 mM 0.0390 mL 0.1952 mL 0.3904 mL 0.9760 mL
    100 mM 0.0312 mL 0.1562 mL 0.3123 mL 0.7808 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Phosphoramide mustard cyclohexanamine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Phosphoramide mustard1566-15-0DNA Alkylator/CrosslinkerDrug Metabolitecyclophosphamidephosphoramide mustardDNAadductdamagerepairgenotoxicityalkylatorInhibitorinhibitorinhibit

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