1. Cell Cycle/DNA Damage
    Metabolic Enzyme/Protease
  2. DNA Alkylator/Crosslinker
    Drug Metabolite
  3. Phosphoramide mustard (cyclohexanamine)

Phosphoramide mustard (cyclohexanamine) 

Cat. No.: HY-137316A Purity: ≥95.0%
Handling Instructions

Phosphoramide mustard cyclohexanamine is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard cyclohexanamine induces DNA damage.

For research use only. We do not sell to patients.

Phosphoramide mustard (cyclohexanamine) Chemical Structure

Phosphoramide mustard (cyclohexanamine) Chemical Structure

CAS No. : 1566-15-0

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5 mg USD 680 In-stock
Estimated Time of Arrival: December 31
10 mg USD 1250 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

Other Forms of Phosphoramide mustard (cyclohexanamine):

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Description

Phosphoramide mustard cyclohexanamine is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard cyclohexanamine induces DNA damage[1][2].

In Vitro

Phosphoramide mustard cyclohexanamine causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication[1].
Phosphoramide mustard cyclohexanamine (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells (SIGCs)[1].
Phosphoramide mustard cyclohexanamine (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage[1].
Phosphoramide mustard cyclohexanamine (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: SIGCs
Concentration: 0.5 μM, 1 μM, 3 μM, 6 μM
Incubation Time: 48 hours
Result: Reduced cell viability at concentrations of 3 μM and higher.

RT-PCR[1]

Cell Line: SIGCs
Concentration: 3 μM, 6 μM
Incubation Time: 24 hours, 48 hours
Result: Increased DDR gene mRNA expression levels.

Western Blot Analysis[1]

Cell Line: SIGCs
Concentration: 3 μM, 6 μM
Incubation Time: 24 hours, 48 hours
Result: Generally increased DDR proteins.
In Vivo

Phosphoramide mustard cyclohexanamine (2.1-20.7 mg/kg; i.p.; daily; for 5 days) inhibits subcutaneous tumor growth in rats[2].
Phosphoramide mustard cyclohexanamine exhibits terminal elimination half-lives (rat 15.1 min) following intravenous administration (rat 59.4 mg/kg)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Rat, subcutaneously implanted Walker 256 carcinosarcoma tumor[2]
Dosage: 2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg
Administration: Intraperitoneal injection, once daily, for 5 consecutive days
Result: Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg.
Animal Model: Rats[2]
Dosage: 86.0 mg/kg (Pharmacokinetic Analysis)
Administration: Intravenous injection
Result: T1/2 (15.1 min).
Molecular Weight

320.20

Formula

C₁₀H₂₄Cl₂N₃O₂P

CAS No.
SMILES

O=P(N(CCCl)CCCl)(N)O.NC1CCCCC1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (156.15 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1230 mL 15.6152 mL 31.2305 mL
5 mM 0.6246 mL 3.1230 mL 6.2461 mL
10 mM 0.3123 mL 1.5615 mL 3.1230 mL
*Please refer to the solubility information to select the appropriate solvent.
References

Purity: ≥95.0%

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Keywords:

Phosphoramide mustard (cyclohexanamine)DNA Alkylator/CrosslinkerDrug Metabolitecyclophosphamidephosphoramide mustardDNAadductdamagerepairgenotoxicityalkylatorInhibitorinhibitorinhibit

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Product Name:
Phosphoramide mustard (cyclohexanamine)
Cat. No.:
HY-137316A
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