Phosphoramide mustard cyclohexanamine

Name: Phosphoramide mustard cyclohexanamine
Brand: MedChemExpress
SKU: HY-137316A
Rating: 4.5 (1 reviews)

Cat. No.: HY-137316A Purity: ≥95.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Phosphoramide mustard cyclohexanamine is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard cyclohexanamine induces DNA damage.

For research use only. We do not sell to patients.

Phosphoramide mustard cyclohexanamine Chemical Structure

CAS No. : 1566-15-0

Size	Price	Stock	Quantity
5 mg	USD 340	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 600	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1250	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2000	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 3200	In-stock	Estimated Time of Arrival: December 31
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500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Phosphoramide mustard cyclohexanamine:

Phosphoramide mustard Get quote

1 Publications Citing Use of MCE Phosphoramide mustard cyclohexanamine

•SSRN. 2024 Mar 21.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Phosphoramide mustard cyclohexanamine is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard cyclohexanamine induces DNA damage^[1]^[2].

In Vitro

Phosphoramide mustard cyclohexanamine causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication^[1].
Phosphoramide mustard cyclohexanamine (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells (SIGCs)^[1].
Phosphoramide mustard cyclohexanamine (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage^[1].
Phosphoramide mustard cyclohexanamine (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	SIGCs
Concentration:	0.5 μM, 1 μM, 3 μM, 6 μM
Incubation Time:	48 hours
Result:	Reduced cell viability at concentrations of 3 μM and higher.

RT-PCR^[1]

Cell Line:	SIGCs
Concentration:	3 μM, 6 μM
Incubation Time:	24 hours, 48 hours
Result:	Increased DDR gene mRNA expression levels.

Western Blot Analysis^[1]

Cell Line:	SIGCs
Concentration:	3 μM, 6 μM
Incubation Time:	24 hours, 48 hours
Result:	Generally increased DDR proteins.

In Vivo

Phosphoramide mustard cyclohexanamine (2.1-20.7 mg/kg; i.p.; daily; for 5 days) inhibits subcutaneous tumor growth in rats^[2].
? Phosphoramide mustard cyclohexanamine exhibits terminal elimination half-lives (rat 15.1 min) following intravenous administration (rat 59.4 mg/kg)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Rat, subcutaneously implanted Walker 256 carcinosarcoma tumor^[2]
Dosage:	2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg
Administration:	Intraperitoneal injection, once daily, for 5 consecutive days
Result:	Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg.

Animal Model:	Rats^[2]
Dosage:	86.0 mg/kg (Pharmacokinetic Analysis)
Administration:	Intravenous injection
Result:	T_1/2 (15.1 min).

Molecular Weight

320.20

Formula

C₁₀H₂₄Cl₂N₃O₂P

CAS No.

1566-15-0

Appearance

Solid

Color

White to off-white

SMILES

O=P(N(CCCl)CCCl)(N)O.NC1CCCCC1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (312.30 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1230 mL	15.6152 mL	31.2305 mL
5 mM	0.6246 mL	3.1230 mL	6.2461 mL
10 mM	0.3123 mL	1.5615 mL	3.1230 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (312.30 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥95.0%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (253 KB) HNMR (141 KB)

Handling Instructions (2659 KB)

References

[1]. Shanthi Ganesan, et al. Phosphoramide mustard exposure induces DNA adduct formation and the DNA damage repair response in rat ovarian granulosa cells. Toxicol Appl Pharmacol. 2015 Feb 1; 282(3): 252–258. [Content Brief]

[2]. S Genka, et al. Brain and plasma pharmacokinetics and anticancer activities of cyclophosphamide and phosphoramide mustard in the rat. Cancer Chemother Pharmacol. 1990;27(1):1-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	3.1230 mL	15.6152 mL	31.2305 mL	78.0762 mL
	5 mM	0.6246 mL	3.1230 mL	6.2461 mL	15.6152 mL
	10 mM	0.3123 mL	1.5615 mL	3.1230 mL	7.8076 mL
	15 mM	0.2082 mL	1.0410 mL	2.0820 mL	5.2051 mL
	20 mM	0.1562 mL	0.7808 mL	1.5615 mL	3.9038 mL
	25 mM	0.1249 mL	0.6246 mL	1.2492 mL	3.1230 mL
	30 mM	0.1041 mL	0.5205 mL	1.0410 mL	2.6025 mL
	40 mM	0.0781 mL	0.3904 mL	0.7808 mL	1.9519 mL
	50 mM	0.0625 mL	0.3123 mL	0.6246 mL	1.5615 mL
	60 mM	0.0521 mL	0.2603 mL	0.5205 mL	1.3013 mL
	80 mM	0.0390 mL	0.1952 mL	0.3904 mL	0.9760 mL
	100 mM	0.0312 mL	0.1562 mL	0.3123 mL	0.7808 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.