1. Cell Cycle/DNA Damage
  2. DNA Alkylator/Crosslinker
  3. Cyclophosphamide

Cyclophosphamide 

Cat. No.: HY-17420 Purity: >98.0%
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Cyclophosphamide is a synthetic alkylating agent chemically related to the nitrogen mustards with antineoplastic activity, a immunosuppressant.

For research use only. We do not sell to patients.

Cyclophosphamide Chemical Structure

Cyclophosphamide Chemical Structure

CAS No. : 50-18-0

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Based on 1 publication(s) in Google Scholar

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Description

Cyclophosphamide is a synthetic alkylating agent chemically related to the nitrogen mustards with antineoplastic activity, a immunosuppressant.

IC50 & Target

DNA Alkylator[1]

In Vitro

Cyclophosphamide induces outer membrane blebbing, leads to DNA fragmentation, as revealed by TUNEL staining of free 3'-OH DNA ends, and induces cleavage of the caspase 3 and caspase 7 substrate PARP in 9L/P450 cells. Bcl-2 expression fully blocks the activation of both initiator caspases as well as the effector caspase 3 in cells treated with activated Cyclophosphamide. Bcl-2 inhibits the cytotoxic effects but not the cytostatic effects of activated Cyclophosphamide[1]. Cyclophosphamide inhibits the AChE reversibly with an IC50 of 511 μM[2]. Carbon tetrachloride does not affect the direct cytotoxicity of cyclophosphamide or 4-hydroxycyclophosphamide to cells in culture[3].

In Vivo

Cyclophosphamide (injected i.p.;2mg/mouse in 0.1 mL PBS, in C3H mice bearing SW1 tumors) increases the percentage of cells that stained for CD3, CD4 or CD8 in both spleens and tumors[4].

Animal Model: Six to eight-week old female C3H/HeN mice bearing SW1 tumors[4]
Dosage: 2 mg/mouse
Administration: Injected i.p.; 2mg/mouse in 0.1 mL PBS; 4 days
Result: Increased the percentage of cells that stained for CD3, CD4 or CD8 in both spleens and tumors.
Molecular Weight

261.09

Formula

C₇H₁₅Cl₂N₂O₂P

CAS No.

50-18-0

SMILES

ClCCN(CCCl)P1(OCCCN1)=O

Shipping

Room temperature in continental US; may vary elsewhere

Storage

4°C, protect from light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

H2O : ≥ 55 mg/mL (210.66 mM)

DMSO : ≥ 38 mg/mL (145.54 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8301 mL 19.1505 mL 38.3010 mL
5 mM 0.7660 mL 3.8301 mL 7.6602 mL
10 mM 0.3830 mL 1.9150 mL 3.8301 mL
*Please refer to the solubility information to select the appropriate solvent.
References

Purity: >98.0%

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Product Name:
Cyclophosphamide
Cat. No.:
HY-17420
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