1. Cell Cycle/DNA Damage
  2. DNA Alkylator/Crosslinker
  3. Cyclophosphamide hydrate

Cyclophosphamide hydrate (Synonyms: Cyclophosphamide monohydrate)

Cat. No.: HY-17420A Purity: >98.0%
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Cyclophosphamide hydrate is a synthetic alkylating agent chemically related to the nitrogen mustards with antineoplastic and immunosuppressive activities.

For research use only. We do not sell to patients.

Cyclophosphamide hydrate Chemical Structure

Cyclophosphamide hydrate Chemical Structure

CAS No. : 6055-19-2

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Based on 1 publication(s) in Google Scholar

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Description

Cyclophosphamide hydrate is a synthetic alkylating agent chemically related to the nitrogen mustards with antineoplastic and immunosuppressive activities.

In Vitro

Cyclophosphamide induces outer membrane blebbing, leads to DNA fragmentation, as revealed by TUNEL staining of free 3'-OH DNA ends, and induces cleavage of the caspase 3 and caspase 7 substrate PARP in 9L/P450 cells. Bcl-2 expression fully blocks the activation of both initiator caspases as well as the effector caspase 3 in cells treated with activated Cyclophosphamide. Bcl-2 inhibits the cytotoxic effects but not the cytostatic effects of activated Cyclophosphamide[1]. Cyclophosphamide inhibits the AChE reversibly with an IC50 of 511 μM[2]. Carbon tetrachloride does not affect the direct cytotoxicity of cyclophosphamide or 4-hydroxycyclophosphamide to cells in culture[3].

In Vivo

Cyclophosphamide (injected i.p.;2mg/mouse in 0.1 mL PBS, in C3H mice bearing SW1 tumors) increases the percentage of cells that stained for CD3, CD4 or CD8 in both spleens and tumors[4].

Animal Model: Six to eight-week old female C3H/HeN mice bearing SW1 tumors[4]
Dosage: 2 mg/mouse
Administration: Injected i.p.; 2mg/mouse in 0.1 mL PBS; Four days
Result: Increased the percentage of cells that stained for CD3, CD4 or CD8 in both spleens and tumors.
Molecular Weight

279.10

Formula

C₇H₁₇Cl₂N₂O₃P

CAS No.

6055-19-2

SMILES

ClCCN(CCCl)P1(OCCCN1)=O.[H]O[H]

Shipping

Room temperature in continental US; may vary elsewhere

Storage
Powder -20°C 3 years
  4°C 2 years

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

H2O : ≥ 50 mg/mL (179.15 mM)

DMSO : ≥ 38 mg/mL (136.15 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5829 mL 17.9147 mL 35.8295 mL
5 mM 0.7166 mL 3.5829 mL 7.1659 mL
10 mM 0.3583 mL 1.7915 mL 3.5829 mL
*Please refer to the solubility information to select the appropriate solvent.
References
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Cyclophosphamide hydrate
Cat. No.:
HY-17420A
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