Pozelimab  (Synonyms: REGN3918)

Pozelimab (REGN3918) is a fully human IgG4 anti-C5 monoclonal antibody. Pozelimab binds to C5 and C5 variants with high affinity and blocks complement-mediated hemolysis. Pozelimab can be used for the research of complement-mediated diseases^[1].

Human IgG4 kappa

Human IgG4 (S228P) kappa, Isotype Control

Human

Complement C5

Pozelimab binds with human C5 and cynomolgus monkey C5 with K_d values of 262 pM and 8.55 nM, respectively at 37˚C, pH7.4^[1].
Pozelimab blocks classic pathway (CP)-mediated hemolysis in normal human and cynomolgus monkey serum with IC₅₀ values of 2.8 and 20 nM, respectively at 37˚C, pH7.4^[1].
Pozelimab blocks alternative pathway (AP)-mediated hemolysis in normal human and cynomolgus monkey serum with IC₅₀ values of 26 and 8.9 nM, respectively at 37˚C, pH7.4^[1].
Pozelimab binds to human C5, cynomolgus monkey C5, human R885C C5 and human C5b,6 with K_d values of 0.189, 2.73, 0.422, 0.465 and 0.137 nM respectively at 25˚C, pH7.4^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pozelimab (15 mg/kg; s.c., once) has a prolonged pharmacokinetic (PK) with a half-life of approximately 13 days in male and female C5^hu/hu mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

727  [NCBI]

P01031

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

144.78 kDa

2096328-94-6

Liquid

Colorless to light yellow

[Pozelimab]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Human IgG4 kappa

• 
  Flow cytometric analysis of 1X10⁶ HepG2 cells with Pozelimab (HY-P99786, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG4 kappa Isotype Control (HY-P99003, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Latuszek A, et al. Inhibition of complement pathway activation with Pozelimab, a fully human antibody to complement component C5. PLoS One. 2020 May 8;15(5):e0231892.  [Content Brief]