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  6. IFN-gamma R1/CD119 Protein, Mouse (HEK293, Fc)

IFN-gamma R1/CD119 Protein, Mouse (HEK293, Fc)

Cat. No.: HY-P73133
Handling Instructions

IFN-gamma R1 (CD119), one of the subunit of IFN-gamma receptor, is a receptor for IFN-gamma. IFN-gamma R1 forms the functional receptor with IFN-gamma R2. Upon binding with IFN-gamma, IFN-gamma R1 and IFN-gamma R2 oligomerize and transphosphorylate. Then, JAK1 and JAK2 are phosphorylated and activated, and STAT1 is recruited to the receptor complex. Phosphorylated STAT1 translocates to the nucleus, where it regulates the expression of IFN-responsive genes (e.g. CD54). IFN-gamma R1/CD119 Protein, Mouse (HEK293, Fc) is a recombinant mouse IFN-gamma R1 (M1-D253) with C-terminal hFc tag, which is produced in HEK293 cells.

For research use only. We do not sell to patients.

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Description

IFN-gamma R1 (CD119), one of the subunit of IFN-gamma receptor, is a receptor for IFN-gamma. IFN-gamma R1 forms the functional receptor with IFN-gamma R2. Upon binding with IFN-gamma, IFN-gamma R1 and IFN-gamma R2 oligomerize and transphosphorylate[1]. Then, JAK1 and JAK2 are phosphorylated and activated, and STAT1 is recruited to the receptor complex. Phosphorylated STAT1 translocates to the nucleus, where it regulates the expression of IFN-responsive genes (e.g. CD54)[2]. IFN-gamma R1/CD119 Protein, Mouse (HEK293, Fc) is a recombinant mouse IFN-gamma R1 (M1-D253) with C-terminal hFc tag, which is produced in HEK293 cells.

Background

IFN-gamma R1 (CD119), one of the subunit of IFN-gamma receptor, is a receptor for IFN-gamma. IFN-gamma R1 is constitutively expressed on the surface of almost all cells[1].
IFN-gamma R1 can associate with IFN-gamma R2 to form a functional receptor. Upon binding with IFN-gamma, IFNγR1 and IFNγR2 oligomerize and transphosphorylate[1]. Then, JAK1 and JAK2 are phosphorylated and activated, and STAT1 is recruited to the receptor complex. The phosphorylation of IFNγR1 creates a docking site for STAT1 and leads to the phosphorylation of STAT1. Phosphorylated STAT1 translocates to the nucleus, where it regulates the expression of IFN-responsive genes (e.g. CD54). IFN-gamma R1 deficiencies are associated with immune responses mediated by IFN-γ, and increased susceptibility to infections. IFN-gamma R1 signaling pathway is important in activating cancer cell death and inhibiting cancer progression[3]
Mouse IFN-gamma R1 consists of extracellular domain (A26-S254), helical domain (I255-Y275), and cytoplasmic domain (W276-S477). The sequence of amino acids in IFNAR1 differs in different species. Mouse IFN-gamma R1 shares 50% aa sequence identity with human. IFN-gamma R1 plays a critical role in antimicrobial, antiviral, and antitumor responses[2].

In Vitro

IFN-gamma R1 (murine, -2 μg/mL, 4 h) suppresses AP(+)-exosome-mediated NO synthesis in RAW264.7 cells[4].

Species

Mouse

Source

HEK293

Tag

C-hFc

Accession

P15261 (M1-D253)

Gene ID

15979  [NCBI]

Molecular Construction
N-term
IFNGR1 (M1-D253)
Accession # P15261
hFc
C-term
Synonyms
Interferon gamma receptor 1; IFN-gamma-R1; CD119; Ifngr1; Ifngr
Molecular Weight

70-75 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
References

IFN-gamma R1/CD119 Protein, Mouse (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IFN-gamma R1/CD119 Protein, Mouse (HEK293, Fc)
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