2. Immunology/Inflammation
  3. Interleukin Related
  4. ST2-IN-1

ST2-IN-1 is a ST2 inhibitor. ST2-IN-1 blocks the binding interaction between ST2 and IL-33, thereby attenuating the downstream ST2/IL-33 signaling pathway. ST2-IN-1 reduces IL-1β release from mast cells and alleviates ST2 upregulation in cells. ST2-IN-1 can be used for research on inflammatory and immune-related diseases.

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ST2-IN-1

ST2-IN-1 화학구조

CAS No. : 3099811-61-4

사이즈 가격 재고 수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 해외재고보유
Solution
10 mM * 1 mL in DMSO 해외재고보유
Solid
5 mg 해외재고보유
10 mg 해외재고보유
25 mg 해외재고보유
50 mg 해외재고보유
100 mg 해외재고보유
200 mg   견적 받기  
500 mg   견적 받기  

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ST2-IN-1 Related Antibodies

Top Publications Citing Use of Products

View All Interleukin Related Isoform Specific Products:

View All Isoforms
IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

  • Biological Activity

  • 순도&문서

  • References

  • 고객리뷰

제품 설명

ST2-IN-1 is a ST2 inhibitor. ST2-IN-1 blocks the binding interaction between ST2 and IL-33, thereby attenuating the downstream ST2/IL-33 signaling pathway. ST2-IN-1 reduces IL-1β release from mast cells and alleviates ST2 upregulation in cells. ST2-IN-1 can be used for research on inflammatory and immune-related diseases[1].

In Vitro

ST2-IN-1 (Compound 31) inhibits the binding of ST2/IL-33 in a cell-free AlphaLISA assay with an IC50 of 7 μM[1].
ST2-IN-1 inhibits ST2/IL-33-mediated NF-κB activation in ST2-overexpressing HEK293 HEKBlue reporter cells, with an IC50 of 7.19 μM[1].
ST2-IN-1 (10-30 μM; 24 h) dose-dependently inhibits IL-1β release from human LAD2 mast cells co-stimulated with IL-33 and 48/80[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ST2-IN-1 Related Antibodies

ELISA Assay[1]

Cell Line: human LAD2 mast cells
Concentration: 10 μM, 30 μM
Incubation Time: 24 h (ST2-IN-1 treatment); 30 min (48/80 stimulation)
Result: Dose-dependently reduced IL-1β release from LAD2 cells co-stimulated with IL-33 and 48/80.
Yielded a partial reduction in IL-1β levels at 10 μM compared to the IL-33 + 48/80 control group.
Yielded a greater reduction in IL-1β levels at 30 μM compared to the IL-33 + 48/80 control group.
분자량

459.58

화학식

C27H33N5O2
CAS No.
Appearance

Solid

Color

Yellow to brown

SMILES

CN(C1=CC=C(C=C1)C2CCCN2CC3=CC=C(N3)C4=CC=C(C(N5CCCC5)=C4)[N+]([O-])=O)C
선적

Room temperature in continental US; may vary elsewhere.
보관
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
용액&용해도
In Vitro: 

DMSO : 100 mg/mL (217.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1759 mL 10.8795 mL 21.7590 mL
5 mM 0.4352 mL 2.1759 mL 4.3518 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • 몰농도 계산기

  • 농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.44 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (5.44 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1759 mL 10.8795 mL 21.7590 mL 54.3975 mL
5 mM 0.4352 mL 2.1759 mL 4.3518 mL 10.8795 mL
10 mM 0.2176 mL 1.0879 mL 2.1759 mL 5.4397 mL
15 mM 0.1451 mL 0.7253 mL 1.4506 mL 3.6265 mL
20 mM 0.1088 mL 0.5440 mL 1.0879 mL 2.7199 mL
25 mM 0.0870 mL 0.4352 mL 0.8704 mL 2.1759 mL
30 mM 0.0725 mL 0.3626 mL 0.7253 mL 1.8132 mL
40 mM 0.0544 mL 0.2720 mL 0.5440 mL 1.3599 mL
50 mM 0.0435 mL 0.2176 mL 0.4352 mL 1.0879 mL
60 mM 0.0363 mL 0.1813 mL 0.3626 mL 0.9066 mL
80 mM 0.0272 mL 0.1360 mL 0.2720 mL 0.6800 mL
100 mM 0.0218 mL 0.1088 mL 0.2176 mL 0.5440 mL
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ST2-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ST2-IN-13099811-61-4Interleukin RelatedILST2 inhibitorHEK293 HEKBlue reporter cellsLAD2 mast cellsInhibitorinhibitorinhibit

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상품명:
ST2-IN-1
Cat. No.:
HY-168532
수량:
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