1. Metabolic Enzyme/Protease
  2. Aminopeptidase
  3. Suc-Ala-Ala-Pro-Leu-pNA

Suc-Ala-Ala-Pro-Leu-pNA  (Synonyms: N-Succinyl-AAPL-p-nitroanilide)

Art. -Nr.: HY-P2648 Reinheit: 99.81%
Handling Instructions Technical Support

Suc-Ala-Ala-Pro-Leu-pNA (N-Succinyl-AAPL-p-nitroanilide) is a tripeptidyl p-nitroanilide substrate that can be catalytically hydrolyzed by acyl amidase-like leucine aminopeptidase in seminal plasma. Amastatin (HY-129298) and Actinonin (HY-113952) exert potent inhibitory effects on this hydrolytic activity. Suc-Ala-Ala-Pro-Leu-pNA can be used for the research of diseases such as azoospermia.

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Kundenspezifische Peptidsynthese

Suc-Ala-Ala-Pro-Leu-pNA

Suc-Ala-Ala-Pro-Leu-pNA Chemische Struktur

CAS. Nr. : 70968-04-6

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Beschreibung

Suc-Ala-Ala-Pro-Leu-pNA (N-Succinyl-AAPL-p-nitroanilide) is a tripeptidyl p-nitroanilide substrate that can be catalytically hydrolyzed by acyl amidase-like leucine aminopeptidase in seminal plasma. Amastatin (HY-129298) and Actinonin (HY-113952) exert potent inhibitory effects on this hydrolytic activity. Suc-Ala-Ala-Pro-Leu-pNA can be used for the research of diseases such as azoospermia[1].

Molekulargewicht

590.63

Formel

C27H38N6O9

CAS. Nr.
Appearance

Solid

Color

White to off-white

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : ≥ 100 mg/mL (169.31 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6931 mL 8.4655 mL 16.9311 mL
5 mM 0.3386 mL 1.6931 mL 3.3862 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Reinheit & Dokumentation
Verweise

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6931 mL 8.4655 mL 16.9311 mL 42.3277 mL
5 mM 0.3386 mL 1.6931 mL 3.3862 mL 8.4655 mL
10 mM 0.1693 mL 0.8466 mL 1.6931 mL 4.2328 mL
15 mM 0.1129 mL 0.5644 mL 1.1287 mL 2.8218 mL
20 mM 0.0847 mL 0.4233 mL 0.8466 mL 2.1164 mL
25 mM 0.0677 mL 0.3386 mL 0.6772 mL 1.6931 mL
30 mM 0.0564 mL 0.2822 mL 0.5644 mL 1.4109 mL
40 mM 0.0423 mL 0.2116 mL 0.4233 mL 1.0582 mL
50 mM 0.0339 mL 0.1693 mL 0.3386 mL 0.8466 mL
60 mM 0.0282 mL 0.1411 mL 0.2822 mL 0.7055 mL
80 mM 0.0212 mL 0.1058 mL 0.2116 mL 0.5291 mL
100 mM 0.0169 mL 0.0847 mL 0.1693 mL 0.4233 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Suc-Ala-Ala-Pro-Leu-pNA
Art. -Nr.:
HY-P2648
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