トリス(ヒドロキシメチル)アミノメタン  (Synonyms: Tris; THAM; Tris(hydroxymethyl)aminomethane)

THAM (Tris) is a low-toxicity amino alcohol buffer, a specific CO₂-consuming proton acceptor that buffers carbon dioxide and acid both in vitro and in vivo. THAM binds protons to form bicarbonate, reduces PaCO₂, and induces intracellular alkalization, thereby ameliorating hypercapnia-induced elevation of pulmonary blood vessels and pulmonary arterial pressure. THAM may cause PaCO₂ rebound, hypoglycemia, and respiratory depression. THAM removes amniotic epithelium and preserves the basement membrane, but depletes extracellular matrix and reduces the adhesion rate of limbal epithelial cells. THAM can act as a CO₂ carrier to enhance the productivity and carbon utilization rate of Scenedesmus obliquus. THAM is a key component of buffer solutions used in various biological, cell culture, biochemical, and molecular biology applications^{[1][2][3][4][5]}.

THAM (5 min) induces intracellular alkalization in human HepG2 hepatocytes perfused with acidotic artificial media, with a larger effect in the presence of low extracellular non-bicarbonate buffer compared to high extracellular non-bicarbonate buffer^[3].
THAM (110 μL) induces intracellular alkalization in human HepG2 hepatocytes bathed in acidotic human blood^[3].
THAM (2-8 mmol/L; 40 min) enhances CO₂ gas absorptivity and total inorganic carbon storage capacity of modified BG-11 medium, with the highest TIC absorption observed at 8 mmol·L^-1[5].
THAM (2-8 mmol/L; 8 days) improves biomass productivity of Scenedesmus dimorphus in indoor shake flask culture, while also increasing polyunsaturated fatty acid content and showing no biodegradation over 8 days^[5].
THAM (6 mmol/L; 8 days) increases biomass areal productivity of Scenedesmus dimorphus by 32-33% and CO₂ utilisation efficiency by 6-12% in outdoor 2 m² raceway pond culture, while also increasing polyunsaturated fatty acid content^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

THAM (weight in kg × 9.2 mEq; i.v.; 1 hour or 3 hours) reduces pulmonary vascular resistance and maintains lower pulmonary arterial pressure in lung-injured piglets with hypercapnia, even after pH rebounds to control levels, while increasing base excess and inducing a post-infusion rebound in PaCO₂^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

121.14

C₄H₁₁NO₃

77-86-1

Solid

White to off-white

OCC(CO)(N)CO

Room temperature in continental US; may vary elsewhere.

Store at room temperature, keep dry and cool

*In solvent : -80°C, 6 months; -20°C, 1 month (Store at room temperature, keep dry and cool)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Nahas GG, et al. Guidelines for the treatment of acidaemia with THAM. Drugs. 1998 Feb;55(2):191-224.  [Content Brief]

  [2]. Höstman S, et al. THAM reduces CO2-associated increase in pulmonary vascular resistance - an experimental study in lung-injured piglets. Crit Care. 2015 Sep 17;19(1):331.  [Content Brief]