2. Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis
  4. HRO761

HRO761  (Synonyms: Werner syndrome RecQ helicase-IN-1)

Cat. No.: HY-148699 Pureté: 99.52%
COA
SDS
Instruction de manipulation Technical Support

HRO761 (Werner syndrome RecQ helicase-IN-1) (example 42) is a potent Werner syndrome RecQ DNA helicase enzyme (WRN) inhibitor and can be used in cancer research.

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CAS No. : 2869954-34-5

Size Prix Stock Quantité
Échantillon gratuit (0.1 - 0.2 mg)   Appliquer maintenant  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
5 mg En stock
10 mg En stock
25 mg En stock
50 mg En stock
100 mg En stock
1 g En stock
5 g   Obtenir un devis  
10 g   Obtenir un devis  

* Veuillez sélectionner la quantité avant d'ajouter des articles.

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Based on 3 publication(s) in Google Scholar

HRO761 Related Antibodies

Top Publications Citing Use of Products
Bio/Physico-chemical Assay
Cell Proliferation/Viability Assay
Cell Imaging/Staining

    HRO761 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 25;16(1):7901.  [Abstract]

    Representative immunofluorescence images and quantification of prometaphase cells showed MiDAS. HRO761 (10 μM; 1.5 h) reduced MiDAS significantly in U2OS cells.

    HRO761 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Dec 31:e2413368.  [Abstract]

    Imaging and relative fluorescence intensity of the ATED probe in HCT116 cells treated with HRO761 (HRO-761) (2–30 μM; 2 h). HRO761 (HRO-761) significantly decreased the intracellular fluorescence signal in a dose-dependent manner, reflecting a progressive decline in WRN enzymatic activity.

    HRO761 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2024 Sep 6;14(1):20824.  [Abstract]

    Graph represented the ratio of mCherry HCT-116 Bd-WRN clone 44 to BFP SW620 Bd-WRN clone 17 at 0 h, 48 h and 96 h after treatment with 0.1% DMSO (UT) or HRO761 (40–400 nM) from four biological repeats.

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    • Activité biologique

    • Pureté et documentation

    • Références

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    Description

    HRO761 (Werner syndrome RecQ helicase-IN-1) (example 42) is a potent Werner syndrome RecQ DNA helicase enzyme (WRN) inhibitor and can be used in cancer research[1].

    IC50 & Target

    Helicase

     

    In Vitro

    HRO761 (Werner syndrome RecQ helicase-IN-1) (Example 42) has an IC50 value of 100 nM for WRN ATPase and GI50 values of 50 nM and >10 μM for SW48 and DLD1 WRN-KO cell lines, respectively[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    HRO761 Related Antibodies
    Essai clinique
    Masse moléculaire

    702.08

    Formule

    C31H31ClF3N9O5
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    O=C(N1CCN(CC1)C2=C(CC)N(CC(NC3=CC=C(C(F)(F)F)C=C3Cl)=O)C4=NC(C5=CCOCC5)=NN4C2=O)C6=NC=NC(C)=C6O
    Livraison

    Room temperature in continental US; may vary elsewhere.
    Stockage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvant et solubilité
    In Vitro: 

    DMSO : 100 mg/mL (142.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.4243 mL 7.1217 mL 14.2434 mL
    5 mM 0.2849 mL 1.4243 mL 2.8487 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Calculateur de molarité

    • Calculateur de dilution

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (3.56 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Pureté et documentation

    Purity: 99.58%

    SDS (394 KB)

    COA (254 KB) HNMR (325 KB) LCMS (96 KB)

    Instruction de manipulation (2659 KB)
    Références

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.4243 mL 7.1217 mL 14.2434 mL 35.6085 mL
    5 mM 0.2849 mL 1.4243 mL 2.8487 mL 7.1217 mL
    10 mM 0.1424 mL 0.7122 mL 1.4243 mL 3.5608 mL
    15 mM 0.0950 mL 0.4748 mL 0.9496 mL 2.3739 mL
    20 mM 0.0712 mL 0.3561 mL 0.7122 mL 1.7804 mL
    25 mM 0.0570 mL 0.2849 mL 0.5697 mL 1.4243 mL
    30 mM 0.0475 mL 0.2374 mL 0.4748 mL 1.1869 mL
    40 mM 0.0356 mL 0.1780 mL 0.3561 mL 0.8902 mL
    50 mM 0.0285 mL 0.1424 mL 0.2849 mL 0.7122 mL
    60 mM 0.0237 mL 0.1187 mL 0.2374 mL 0.5935 mL
    80 mM 0.0178 mL 0.0890 mL 0.1780 mL 0.4451 mL
    100 mM 0.0142 mL 0.0712 mL 0.1424 mL 0.3561 mL
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    HRO761 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    HRO7612869954-34-5Werner syndrome RecQ helicase-IN-1HRO 761HRO-761DNA/RNA SynthesisWRNcancerInhibitorinhibitorinhibit

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