1. Cell Cycle/DNA Damage
    Metabolic Enzyme/Protease
  2. Nucleoside Antimetabolite/Analog
    Endogenous Metabolite
  3. 8-Azaguanine

8-Azaguanine 

Cat. No.: HY-B1468 Purity: ≥98.0%
Handling Instructions

8-Azaguanine is a purine analogue that shows antineoplastic activity. 8-Azaguanine functions as an antimetabolite and easily incorporates into ribonucleic acids, interfering with normal biosynthetic pathways, thus inhibiting cellular growth.

For research use only. We do not sell to patients.

8-Azaguanine Chemical Structure

8-Azaguanine Chemical Structure

CAS No. : 134-58-7

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 55 In-stock
Estimated Time of Arrival: December 31
Solid
100 mg USD 50 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE 8-Azaguanine

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

8-Azaguanine is a purine analogue that shows antineoplastic activity. 8-Azaguanine functions as an antimetabolite and easily incorporates into ribonucleic acids, interfering with normal biosynthetic pathways, thus inhibiting cellular growth[1].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

8-Azaguanine induces a decrease in cell viability in a dose, time and cell type dependent manner with MOLT3 cells being the most sensitive to 8-Azaguanine citotoxic effects (24h IC50: 10 μM) when compared with CEM cells (24h IC50: 100 μM). MOLT3 cell treated with 8-Azaguanine shows an increase in CD26 expression (MIF) compared with that of CEM cell submitted to the same conditions (65.4 versus 18.7)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

152.11

Formula

C₄H₄N₆O

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 2.44 mg/mL (16.04 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.5742 mL 32.8709 mL 65.7419 mL
5 mM 1.3148 mL 6.5742 mL 13.1484 mL
10 mM 0.6574 mL 3.2871 mL 6.5742 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 0.24 mg/mL (1.58 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.24 mg/mL (1.58 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 0.24 mg/mL (1.58 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[1]

Detection of apoptosis and necrosis is analyzed by Annexin V and propidium iodide staining. After incubation in the absence or in the presence of 8-Azaguanine, cells are washed (centrifuged at 300×g during 5 min) and incubated for 10 min at 4°C in 440 μL Annexin buffer, containing 5 μL Annexin V and 5 μL propidium iodide. Then cells are washed and re-suspended in phosphate buffered saline (PBS) until analysis[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: ≥98.0%

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Product Name:
8-Azaguanine
Cat. No.:
HY-B1468
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