1. Cell Cycle/DNA Damage
  2. Nucleoside Antimetabolite/Analog


Cat. No.: HY-B1468 Purity: >98.0%
Handling Instructions

8-Azaguanine is a purine analogue which shows antineoplastic activity.

For research use only. We do not sell to patients.

8-Azaguanine Chemical Structure

8-Azaguanine Chemical Structure

CAS No. : 134-58-7

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
100 mg USD 50 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References


8-Azaguanine is a purine analogue which shows antineoplastic activity.

In Vitro

8-Azaguanine is a purine analogue which shows antineoplastic activity. The results show that cell viability decreases after incubation with 8-Azaguanine, in a dose and time dependent manner. After 24 h of treatment with 8-Azaguanine the IC50 for CEM cell line is about 100 μM while for MOLT3 cell line the IC50 is about 10 μM. CEM and MOLT3 cells surface expression of CD26 increases after treatment with 8-Azaguanine in a concentrations dependent manner[1].

Solvent & Solubility
In Vitro: 

DMSO : 6 mg/mL (39.45 mM; Need ultrasonic and warming)

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.5742 mL 32.8709 mL 65.7419 mL
5 mM 1.3148 mL 6.5742 mL 13.1484 mL
10 mM 0.6574 mL 3.2871 mL 6.5742 mL
*Please refer to the solubility information to select the appropriate solvent.
Cell Assay

Detection of apoptosis and necrosis is analyzed by Annexin V and propidium iodide staining. After incubation in the absence or in the presence of 8-Azaguanine, cells are washed (centrifuged at 300×g during 5 min) and incubated for 10 min at 4°C in 440 μL Annexin buffer, containing 5 μL Annexin V and 5 μL propidium iodide. Then cells are washed and re-suspended in phosphate buffered saline (PBS) until analysis[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight








Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month

Room temperature in continental US; may vary elsewhere

Purity: >98.0%

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Cat. No.: HY-B1468