BAY-2402234

Name: BAY-2402234
Brand: MedChemExpress
SKU: HY-112645
Rating: 5.0 (11 reviews)

Cat. No.: HY-112645 Pureza: 99.90%: Ficha de datos
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BAY-2402234 is a selective dihydroorotate dehydrogenase (DHODH) inhibitor for the treatment of myeloid malignancies.

Para uso exclusivo en investigación. No vendemos a pacientes.

No. CAS : 2225819-06-5

Tamaño	Stock	Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
1 mg	En stock	Estimated Time of Arrival: December 31
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

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Revisión del cliente

Based on 11 publication(s) in Google Scholar

11 Publications Citing Use of MCE BAY-2402234

In Vivo Efficacy Study

Bio/Physico-chemical Assay

: BAY-2402234 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 4;16(1):9664. [Abstract]; BAY-2402234 (2-5 mg/kg; i.g.; once daily for 7 d) resulted in a halt in body weight increase of C57BL/6J mice post-PHx.

: BAY-2402234 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 4;16(1):9664. [Abstract]; The DHODH inhibitor BAY-2402234 (2 mg/kg; i.g.; once daily for 7 d) blocked the full restoration of the liver in C57BL/6J mice post-PHx, as indicated by the liver-to-body weight ratio (LBWR).

: BAY-2402234 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 4;16(1):9664. [Abstract]; Assessment of proliferation using western blot of MCM2, PCNA, and pHH3 in ～35% PHx mice with or without BAY-2402234 (2 mg/kg; i.g.; once daily for 14 d).

: BAY-2402234 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 4;16(1):9664. [Abstract]; BAY-2402234 (2 mg/kg; i.g.; once daily for 7 d) considerably suppressed DHODH-dependent respiration and DHODH activity in mice subjected to ～ 35% PHx.

: BAY-2402234 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 4;16(1):9664. [Abstract]; BAY-2402234 (2 mg/kg; i.g.; once daily for 3 d) considerably lowered the orotate-to-DHO ratio in mice subjected to ～ 35% PHx.

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Revisión del cliente

Descripciòn

BAY-2402234 is a selective dihydroorotate dehydrogenase (DHODH) inhibitor for the treatment of myeloid malignancies.

IC₅₀ & Target

DHODH^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
L02	IC₅₀	1.1 nM Compound: BAY; BAY240223	Cytotoxicity against human L02 cells assessed as cell growth inhibition incubated for 96 hrs by MTT assay Cytotoxicity against human L02 cells assessed as cell growth inhibition incubated for 96 hrs by MTT assay	[PMID: 34905371]
Raji	IC₅₀	0.4 nM Compound: BAY; BAY240223	Antiproliferative activity against human Raji cells assessed as cell growth inhibition incubated for 96 hrs by MTT assay Antiproliferative activity against human Raji cells assessed as cell growth inhibition incubated for 96 hrs by MTT assay	[PMID: 34905371]
THP-1	EC₅₀	0.0024 μM Compound: BAY-2402234	Induction of cell differentiation in human THP-1 cells assessed as CD14 expression after 3 days by flow cytometric analysis Induction of cell differentiation in human THP-1 cells assessed as CD14 expression after 3 days by flow cytometric analysis	[PMID: 33844533]
THP-1	EC₅₀	0.0034 μM Compound: BAY-2402234	Induction of apoptosis in human THP-1 cells after 3 days by Annexin-V-FITC staining based flow cytometry Induction of apoptosis in human THP-1 cells after 3 days by Annexin-V-FITC staining based flow cytometry	[PMID: 33844533]

In Vitro

BAY-2402234 is a selective low-nanomolar inhibitor of human DHODH enzymatic activity. In vitro, it potently inhibits proliferation of AML cell lines in the sub-nanomolar to low-nanomolar range. BAY-2402234 induces differentiation of AML cell lines also in a sub-nanomolar to low-nanomolar range, demonstrating the anticipated mode of action in cellular mechanistic assays^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

BAY-2402234 exhibits strong in vivo anti-tumor efficacy in monotherapy in several subcutaneous and disseminated AML xenografts as well as AML patient-derived xenograft (PDX) models. Target engagement of the novel DHODH inhibitor BAY-2402234 can be observed by increase of tumoral and plasma dihydroorotate levels after treatment with the inhibitor. Consistent with the in vitro data BAY-2402234 induces AML differentiation in vivo as detected by upregulation of differentiation cell surface markers in xenograft and PDX models after treatment with the inhibitor. Furthermore, differentiation-associated transcriptomic changes are evident following a single administration of BAY-2402234 in vivo^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ensayo clínico

Peso molecular

520.84

Fòrmula

C₂₁H₁₈ClF₅N₄O₄

No. CAS

2225819-06-5

Appearance

Solid

Color

White to light yellow

SMILES

FC1=C(NC(C2=CC(F)=C(N3N=C(CO)N(CC)C3=O)C=C2O[C@@H](C)C(F)(F)F)=O)C(Cl)=CC=C1

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvente y solubilidad

In Vitro:

DMSO : 125 mg/mL (240.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9200 mL	9.5999 mL	19.1998 mL
5 mM	0.3840 mL	1.9200 mL	3.8400 mL
10 mM	0.1920 mL	0.9600 mL	1.9200 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 99.90%

Select Batch:

Ficha de datos (278 KB) SDS (396 KB)

COA (255 KB) HNMR (291 KB) RP-HPLC (247 KB) MS (68 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Andreas Janzer, et al. Abstract DDT02-04: BAY 2402234: A novel, selective dihydroorotate dehydrogenase (DHODH) inhibitor for the treatment of myeloid malignancies. AACR Annual Meeting 2018; April 14-18, 2018; Chicago, IL.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9200 mL	9.5999 mL	19.1998 mL	47.9994 mL
	5 mM	0.3840 mL	1.9200 mL	3.8400 mL	9.5999 mL
	10 mM	0.1920 mL	0.9600 mL	1.9200 mL	4.7999 mL
	15 mM	0.1280 mL	0.6400 mL	1.2800 mL	3.2000 mL
	20 mM	0.0960 mL	0.4800 mL	0.9600 mL	2.4000 mL
	25 mM	0.0768 mL	0.3840 mL	0.7680 mL	1.9200 mL
	30 mM	0.0640 mL	0.3200 mL	0.6400 mL	1.6000 mL
	40 mM	0.0480 mL	0.2400 mL	0.4800 mL	1.2000 mL
	50 mM	0.0384 mL	0.1920 mL	0.3840 mL	0.9600 mL
	60 mM	0.0320 mL	0.1600 mL	0.3200 mL	0.8000 mL
	80 mM	0.0240 mL	0.1200 mL	0.2400 mL	0.6000 mL
	100 mM	0.0192 mL	0.0960 mL	0.1920 mL	0.4800 mL

BAY-2402234 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BAY-24022342225819-06-5BAY2402234BAY 2402234Dihydroorotate DehydrogenaseDNA/RNA SynthesisDHODHInhibitorinhibitorinhibit

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BAY-2402234

Revisión del cliente

BAY-2402234 Related Antibodies

11 Publications Citing Use of MCE BAY-2402234

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas DNA/RNA Synthesis:

Actividad biológica

Pureza y Documentación

Referencias

Revisión del cliente

BAY-2402234 Related Antibodies

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

BAY-2402234 Related Classifications

Keywords:

Productos vistos recientemente:

Revisión del cliente

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