(S)-(-)-Bay-K-8644
Based on 9 publication(s) in Google Scholar
(S)-(-)-Bay-K-8644 is an agonist of L-type Ca2+ channel. (S)-(-)-Bay-K-8644 activates Ba2+ currents (IBa) (EC50=32 nM).
For research use only. We do not sell to patients.
- Purity : 99.22%
- CAS No.: 98625-26-4
- Formula: C16H15F3N2O4
- Molecular Weight:356.30
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) (S)-(-)-Bay-K-8644
More- Cell Discov. 2024 Nov 12;10(1):114. [Abstract]
- Cell Death Discov. 2025 Oct 27;11(1):492. [Abstract]
- Free Radic Biol Med. 2024 Mar:213:150-163. [Abstract]
- Cell Rep. 2024 Dec 10;43(12):115074. [Abstract]
- Br J Pharmacol. 2025 May 13. [Abstract]
- Life Sci. 2019 Mar 15:221:135-142. [Abstract]
- Eur J Pharmacol. 2020 Nov 5:886:173513. [Abstract]
- Int J Mol Sci. 2023 Nov 27;24(23):16806. [Abstract]
- Exp Cell Res. 2025 Apr 1;447(1):114515. [Abstract]
All Calcium Channel Isoforms
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Biological Activity
Description
IC50 & Target
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L-type calcium channel |
In Vitro
(±)-Bay K 8644, a conventional racemic mixture of Bay K 8644, is widely used as an L-type Ca2+ channel agonist. Each optical isomer possesses opposite effects on IBa (R(+)-Bay K 8644 as an antagonist and (S)-(-)-Bay-K-8644 as an agonist. (S)-(-)-Bay-K-8644 can prevent the inhibitory actions of two distinct cyclic nucleotide pathways on IBa in gastric myocytes of the guinea pig antrum[1]. The Ca2+ channel activity is enhanced by 3–30 μM (S)-(-)-Bay-K-8644 an agonist of L-type Ca2+ channels[2]. The interactions of two Ca2+ channel activators (S)-(-)-Bay-K-8644 and FPL 64176 is examined on smooth muscle L-type Ca2+ channels. FPL 64176 (300 nM) causes a sustained contraction of rat tail artery strips. This contractile response is inhibited by approximately 70% by (S)-(-)-Bay-K-8644 (EC50=14 nM). (S)-(-)-Bay-K-8644 (100 nM) increases whole-cell Ca2+ currents in A7r5 smooth muscle cells but effectively blocks further stimulation by 1 μM FPL 64176[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 98625-26-4
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Appearance Solid
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Molecular Weight 356.30
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Formula C16H15F3N2O4
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Color Light yellow to yellow
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SMILES
CC1=C([N+]([O-])=O)[C@@H](C2=C(C(F)(F)F)C=CC=C2)C(C(OC)=O)=C(C)N1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (9)
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Journal Impact Factor
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Most Recent
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Cell Discov
Cancer cells sense solid stress to enhance metastasis by CKAP4 phase separation-mediated microtubule branching. [Abstract]2024 Nov 12;10(1):114. PMID: 39528501 -
Cell Death Discov
Amlodipine exerts inhibitory effects against glioma stem cells through degrading EGFR and down-regulating its downstream pro-survival pathways. [Abstract]2025 Oct 27;11(1):492. PMID: 41145413 -
Free Radic Biol Med
Quercetin induces ferroptosis in gastric cancer cells by targeting SLC1A5 and regulating the p-Camk2/p-DRP1 and NRF2/GPX4 Axes. [Abstract]2024 Mar:213:150-163. PMID: 38190923 -
Cell Rep
Adiponectin receptor 1-mediated basolateral amygdala-prelimbic cortex circuit regulates methamphetamine-associated memory. [Abstract]2024 Dec 10;43(12):115074. PMID: 39661515 -
Br J Pharmacol
Contributions of synaptic energetic dysfunction by microtubule dynamics and microtubule-based mitochondrial transport disorder to morphine tolerance. [Abstract]2025 May 13. PMID: 40361281 -
Life Sci
2019 Mar 15:221:135-142. PMID: 30731142 -
Eur J Pharmacol
L-type calcium channel blockers decrease the iron overload-mediated oxidative stress in renal epithelial cells by reducing iron accumulation. [Abstract]2020 Nov 5:886:173513. PMID: 32898550 -
Int J Mol Sci
AsHC 360 Exposure Influence on Epileptiform Discharges in Hippocampus of Infantile Male Rats In Vitro. [Abstract]2023 Nov 27;24(23):16806. PMID: 38069126 -
Exp Cell Res
Investigating the effects of TRPV4 and Cav1.2 channels in 3D culture for promoting the differentiation of BMSCs at various stages. [Abstract]2025 Apr 1;447(1):114515. PMID: 40073957
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (280.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (7.02 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.84 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Zhu HL, et al. Antagonistic actions of S(-)-Bay K 8644 on cyclic nucleotide-induced inhibition of voltage-dependent Ba(2+) currents in guinea pig gastric antrum. Naunyn Schmiedebergs Arch Pharmacol. 2008 Dec;378(6):609-15. [Content Brief]
[2]. Mironov SL, et al. L-type Ca2+ channels in inspiratory neurones of mice and their modulation by hypoxia. J Physiol. 1998 Oct 1;512 ( Pt 1):75-87. [Content Brief]
[3]. Rampe D, et al. Functional interactions between two Ca2+ channel activators, (S)-Bay K 8644 and FPL 64176, in smooth muscle. Mol Pharmacol. 1992 Apr;41(4):599-602. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8066 mL | 14.0331 mL | 28.0662 mL | 70.1656 mL |
| 5 mM | 0.5613 mL | 2.8066 mL | 5.6132 mL | 14.0331 mL | |
| 10 mM | 0.2807 mL | 1.4033 mL | 2.8066 mL | 7.0166 mL | |
| 15 mM | 0.1871 mL | 0.9355 mL | 1.8711 mL | 4.6777 mL | |
| 20 mM | 0.1403 mL | 0.7017 mL | 1.4033 mL | 3.5083 mL | |
| 25 mM | 0.1123 mL | 0.5613 mL | 1.1226 mL | 2.8066 mL | |
| 30 mM | 0.0936 mL | 0.4678 mL | 0.9355 mL | 2.3389 mL | |
| 40 mM | 0.0702 mL | 0.3508 mL | 0.7017 mL | 1.7541 mL | |
| 50 mM | 0.0561 mL | 0.2807 mL | 0.5613 mL | 1.4033 mL | |
| 60 mM | 0.0468 mL | 0.2339 mL | 0.4678 mL | 1.1694 mL | |
| 80 mM | 0.0351 mL | 0.1754 mL | 0.3508 mL | 0.8771 mL | |
| 100 mM | 0.0281 mL | 0.1403 mL | 0.2807 mL | 0.7017 mL |
(S)-(-)-Bay-K-8644 Related Classifications
HY-15124 Related Classifications
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