2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Benzo[a]pyrene

Benzo[a]pyrene  (Synonyms: 3,4-Benzopyrene)

Cat. No.: HY-107377 Purity: 99.79%
COA Handling Instructions

Benzo[a]pyrene shows lung carcinogenicity in animal models, and it is frequently used in chemoprevention studies.

For research use only. We do not sell to patients.

Benzo[a]pyrene Chemical Structure

Benzo[a]pyrene Chemical Structure

CAS No. : 50-32-8

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Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
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Solid
5 mg USD 38 In-stock
10 mg USD 60 In-stock
25 mg USD 120 In-stock
50 mg USD 200 In-stock
100 mg USD 320 In-stock
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Based on 2 publication(s) in Google Scholar

Benzo[a]pyrene Related Antibodies

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Description

Benzo[a]pyrene shows lung carcinogenicity in animal models, and it is frequently used in chemoprevention studies.

In Vivo

Statistically significant decrease is observed at 7 weeks in females receiving 1.0 mg Benzo[a]pyrene (B[a]P) compare with the vehicle group. As lung tumorigenesis induced by Benzo[a]pyrene is dose dependent in female A/J mice. The incidence of hyperplasia values in females treating with 0.25, 0.50, and 1.0 mg Benzo[a]pyrene are significantly higher than in the vehicle-treated group. The incidence of adenoma in females receiving 1.0 mg Benzo[a]pyrene is significantly higher than in the vehicle group. The multiplicity of hyperplasia in females receiving 0.50 or 1.0 mg Benzo[a]pyrene is significantly higher than in the vehicle group. The multiplicity of adenoma in the group treated with 1.0 mg is also significantly higher than in the vehicle group. The incidences of hyperplasia and adenoma in female A/J mice are significantly increased by Benzo[a]pyrene in a dose-dependent manner[1].
Benzo[a]pyrene induces an average of 9.38±1.75 tumors with an average tumor load of 19.53±3.81 mm3 (P<0.05 compare to control). Benzo[a]pyrene administration significantly (P<0.05) decreases cAMP levels in tumors with adjacent lung tissues. The expression level of PDE4D gene is also increased by Benzo[a]pyrene administration[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

252.31

Appearance

Solid

Formula

C20H12
CAS No.
SMILES

C12=C(C=C3)C=C(C=CC=C4)C4=C1C=CC5=CC=CC3=C25
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 25 mg/mL (99.08 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9634 mL 19.8169 mL 39.6338 mL
5 mM 0.7927 mL 3.9634 mL 7.9268 mL
10 mM 0.3963 mL 1.9817 mL 3.9634 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  1% CMC-Na/saline water

    Solubility: 5 mg/mL (19.82 mM); Suspended solution; Need ultrasonic and warming and heat to 50°C

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 1.67 mg/mL (6.62 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 1.67 mg/mL (6.62 mM); Suspended solution; Need ultrasonic

  • 4.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 1.67 mg/mL (6.62 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.79%

COA (253 KB) HNMR (134 KB) RP-HPLC (253 KB)

Handling Instructions (2659 KB)
References
Animal Administration
[2]

Female A/J mice are randomized into eight groups (n=8): (i) control; (ii)Benzo[a]pyrene (B(a)P)+vehicle (methocel); (iii) Benzo[a]pyrene+roflumilast 1 mg/kg; (iv) Benzo[a]pyrene+roflumilast 5 mg/kg; (v) Benzo[a]pyrene+aerozolie phosphate-buffer saline (PBS); (vi) Benzo[a]pyrene+aerosolize budesonide 2.25 mg/mL; (vii) Benzo[a]pyrene+aerosolized budesonide 2.25 mg/mL+roflumilast 1 mg/kg; and (viii) Benzo[a]pyrene+aerosolize budesonide 2.25 mg/mL+roflumilast 5 mg/kg groups. A single dose of Benzo[a]pyrene in corn oil is given intraperitoneally once at 100 mg/kg body weight. Roflumilast (1 or 5 mg/kg) is started 2 weeks after Benzo[a]pyrene. It is continued for 26 weeks (3 days/week) via oral gavage. Mice in the Benzo[a]pyrene+vehicle group are treated with an equal volume of methocel as solvent control. Aerosolizing budesonide is administrated by inhaling route as an aerosol at a dose of 2.25 mg/mL for 2 min per application at 2 weeks after Benzo[a]pyrene. It is continued for 26 weeks (5 days/week). PBS is also used as solvent control by inhaling route after Benzo[a]pyrene administration in the Benzo[a]pyrene+PBS group. Mice are killed at 28 weeks after exposure to Benzo[a]pyrene. Their lungs are excised and stored at -70 °C[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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Benzo[a]pyrene Related Classifications

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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

Benzo[a]pyrene50-32-83,4-BenzopyreneEndogenous MetaboliteInhibitorinhibitorinhibit

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