C-176

Name: C-176
Brand: MedChemExpress
SKU: HY-112906
Rating: 5.0 (110 reviews)

製品番号: HY-112906 純度: 99.45%: Data Sheet SDS COA 取扱説明書
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C-176 is a selective and blood-brain barrier permeable STING inhibitor. C-176 covalently targets transmembrane cysteine residue 91 and thereby blocking activation-induced palmitoylation of STING.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 314054-00-7

容量	価格（税別）	在庫状況	数量

>無料サンプル (0.1 - 0.2 mg)		今すぐ申し込む
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 39	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 39	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$36	在庫あり	Estimated Time of Arrival: December 31
10 mg	$50	在庫あり	Estimated Time of Arrival: December 31
25 mg	$100	在庫あり	Estimated Time of Arrival: December 31
50 mg	$170	在庫あり	Estimated Time of Arrival: December 31
100 mg	$250	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 110 publication(s) in Google Scholar

顧客検証

: C-176 purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2020 May 25;17(1):165. [Abstract]; Western blotting indicated that the level of TBK1 phosphorylation is significantly increased after SAH. Compared with SAH + vehicle group, the phosphorylation of TBK1 is remarkably inhibited by C-176.

: C-176 purchased from MedChemExpress. Usage Cited in: Aging Cell. 2020 Aug;19(8):e13186. [Abstract]; The BMDMs from the young and aged mice are pretreated with C-176 (20 µM) or STING siRNA followed by stimulation with mtDNA (100 ng/ml). The C-176 treatment effectively inhibits STING activation in both young and aged BMDMs post-mtDNA stimulation, as shown by the decreased protein levels of P-STING and P-TBK1.

: C-176 purchased from MedChemExpress. Usage Cited in: Aging Cell. 2020 Aug;19(8):e13186. [Abstract]; The BMDMs from the young and aged mice are pretreated with C-176 (20 µM) or STING siRNA followed by stimulation with mtDNA (100 ng/ml). STING inhibition by C-176 blocks the over-activation of NLRP3 signaling in the BMDMs from the young and aged mice post-mtDNA stimulation, as shown by decreased levels of NLRP3 and Cleaved caspase-1 expression.

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製品説明

IC₅₀ & Target

STING^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HCC1143	IC₅₀	9.5 μM Compound: 1	Cytotoxicity against human HCC1143 cells assessed as reduction in cell viability incubated for 3 days by cell-titer-Glo reagent based assay Cytotoxicity against human HCC1143 cells assessed as reduction in cell viability incubated for 3 days by cell-titer-Glo reagent based assay	[PMID: 26116180]
HCC1806	IC₅₀	6.2 μM Compound: 1	Cytotoxicity against human HCC1806 cells assessed as reduction in cell viability incubated for 3 days by cell-titer-Glo reagent based assay Cytotoxicity against human HCC1806 cells assessed as reduction in cell viability incubated for 3 days by cell-titer-Glo reagent based assay	[PMID: 26116180]
HCC38	IC₅₀	8.7 μM Compound: 1	Cytotoxicity against human HCC38 cells assessed as reduction in cell viability incubated for 3 days by cell-titer-Glo reagent based assay Cytotoxicity against human HCC38 cells assessed as reduction in cell viability incubated for 3 days by cell-titer-Glo reagent based assay	[PMID: 26116180]
RAW264.7	IC₅₀	1.14 μM Compound: C-176	Anti inflammatory activity in LPS-induced mouse RAW264.7 cells assessed as inhibition of NO production preincubated with compound for 2 hrs followed by LPS stimulation and measured after 24 hrs by griess reagent based assay Anti inflammatory activity in LPS-induced mouse RAW264.7 cells assessed as inhibition of NO production preincubated with compound for 2 hrs followed by LPS stimulation and measured after 24 hrs by griess reagent based assay	[PMID: 36228412]

体外実験

C-176 strongly reduces STING-mediated, but not RIG-I- or TBK1-mediated, IFNβ reporter activity. Pretreatment with C-176 markedly reduce the CMA-mediated induction of serum levels of type I IFNs and IL-6^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

C-176 (750/375 nmol C-176 per mouse in 200 μL corn oil) significantly reduces the CMA-mediated induction of serum levels of type I IFNs and IL-6., without significant toxicity^[1].
C-176 results in a significant reduction in serum levels of type I IFNs and in a strong suppression of inflammatory parameters in the heart, with no evident signs of overt toxicity Trex1^/ mice^[1].
C-176 demonstrates marked amelioration of various signs of systemic inflammation in Trex1^/ mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	WT type mice.
Dosage:	750/375 nmol C-176 per mouse in 200 μL corn oil (~1.34/0.67 mg/mL).
Administration:	Intraperitoneally, once.
Result:	Significantly reduced Serum levels of type I IFNs and IL-6.

分子量

358.09

分子式

C₁₁H₇IN₂O₄

CAS 番号

314054-00-7

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=CC=C([N+]([O-])=O)O1)NC2=CC=C(I)C=C2

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 62.5 mg/mL (174.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 2.5 mg/mL (6.98 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7926 mL	13.9630 mL	27.9259 mL
5 mM	0.5585 mL	2.7926 mL	5.5852 mL
10 mM	0.2793 mL	1.3963 mL	2.7926 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 1.67 mg/mL (4.66 mM); Suspended solution; Need ultrasonic and warming

This protocol yields a suspended solution of 1.67 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.25 mg/mL (0.70 mM); Clear solution

This protocol yields a clear solution of ≥ 0.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (2.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.25 mg/mL (0.70 mM); Clear solution

This protocol yields a clear solution of ≥ 0.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (2.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 4

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: 0.25 mg/mL (0.70 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (2.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.45%

Select Batch:

データシート (275 KB) SDS (393 KB)

COA (247 KB) HNMR (257 KB) LCMS (101 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Haag SM, et al. Targeting STING with covalent small-molecule inhibitors. Nature. 2018 Jul;559(7713):269-273. [Content Brief]

[2]. Zhang LM, et al. STING mediates neuroinflammatory response by activating NLRP3-related pyroptosis in severe traumatic brain injury. J Neurochem. 2022 Sep;162(5):444-462. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	2.7926 mL	13.9630 mL	27.9259 mL	69.8149 mL
Ethanol / DMSO	5 mM	0.5585 mL	2.7926 mL	5.5852 mL	13.9630 mL
DMSO	10 mM	0.2793 mL	1.3963 mL	2.7926 mL	6.9815 mL
	15 mM	0.1862 mL	0.9309 mL	1.8617 mL	4.6543 mL
	20 mM	0.1396 mL	0.6981 mL	1.3963 mL	3.4907 mL
	25 mM	0.1117 mL	0.5585 mL	1.1170 mL	2.7926 mL
	30 mM	0.0931 mL	0.4654 mL	0.9309 mL	2.3272 mL
	40 mM	0.0698 mL	0.3491 mL	0.6981 mL	1.7454 mL
	50 mM	0.0559 mL	0.2793 mL	0.5585 mL	1.3963 mL
	60 mM	0.0465 mL	0.2327 mL	0.4654 mL	1.1636 mL
	80 mM	0.0349 mL	0.1745 mL	0.3491 mL	0.8727 mL
	100 mM	0.0279 mL	0.1396 mL	0.2793 mL	0.6981 mL

C-176 Related Classifications

Inflammation/Immunology

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

C-176314054-00-7C176C 176STINGStimulator of Interferon GenesTMEM173MITAERISMPYSInhibitorinhibitorinhibit

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C-176

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C-176 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

C-176 Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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