2. Metabolic Enzyme/Protease Autophagy
  3. HIF/HIF Prolyl-Hydroxylase Autophagy
  4. DMOG

DMOG  (Synonyms: Dimethyloxallyl Glycine)

製品番号: HY-15893 純度: 99.93%
COA 取扱説明書 Technical Support

DMOG (Dimethyloxallyl Glycine) is a cell permeable and competitive inhibitor of HIF-PH, which results in HIF-1α stabilisation and accmulation?in vitro and in vivo. DMOG is an α-ketoglutarate analogue and inhibits α-KG-dependent hydroxylases. DMOG?acts as a pro-angiogenic agent and plays a protective role in experimental model of colitis and diarrhoea via HIF-1 related signal. DMOG induces cell autophagy.

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CAS 番号 : 89464-63-1

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>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
50 mg $79 在庫あり
100 mg $125 在庫あり
200 mg $200 在庫あり
500 mg $400 在庫あり
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カスタマーレビュー

Based on 76 publication(s) in Google Scholar

Other Forms of DMOG:

DMOG 関連抗体

Top Publications Citing Use of Products

顧客検証

Histological Imaging/Staining
WB
RT-PCR

    DMOG purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May 24:e2414504.  [Abstract]

    NRCMs were administered with 1 mM DMOG for 24 h, and the protein levels of NEU1 as well as HIF1α protein expression were measured by western blot.

    DMOG purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May 20:e17128.  [Abstract]

    Male PCMKO mice were administered with AAV-PCSK9DY, followed by HFD feeding for 12 weeks and injected with HIF-1 stabilizer DMOG (8 mg per mouse) or saline for the last 3 weeks (n = 8). BMDMs of PCMKO mice were collected for examination. Representative images and collagen quantification of the aortic roots stained with Masson staining were shown. Scale bars: 250 μm.

    DMOG purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May 24:e2414504.  [Abstract]

    NRCMs were administered with 1 μM DOX and/or 1 mM DMOG for 24 h, and the mRNA levels of NEU1 were measured by RT-qPCR (n = 3).

    DMOG purchased from MedChemExpress. Usage Cited in: Front Immunol. 2018 Jul 23:9:1667.  [Abstract]

    1 mM DMOG treatment for 8 h was used to upregulate the HIF-1α protein level in NR8383 cells.

    DMOG purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2015 Jan 16;290(3):1389-94.  [Abstract]

    The hypoxia induced the expression of NEK8. Cells are treated with CoCl2 (100 μM) or DMOG (100 μM) for 6 h or cultured for 12 h in hypoxia (1.0% oxygen). HIF-1α and NEK8 are analyzed by immunoblotting, GAPDH is employed as a loading control.

    HIF/HIF Prolyl-Hydroxylase アイソフォーム固有の製品をすべて表示:

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    HIF-1α HIF

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    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    DMOG (Dimethyloxallyl Glycine) is a cell permeable and competitive inhibitor of HIF-PH, which results in HIF-1α stabilisation and accmulation?in vitro and in vivo[1]. DMOG is an α-ketoglutarate analogue and inhibits α-KG-dependent hydroxylases. DMOG?acts as a pro-angiogenic agent and plays a protective role in experimental model of colitis and diarrhoea via HIF-1 related signal[2][4]. DMOG induces cell autophagy[5].

    IC50 & Target

    HIF-1α prolyl hydroxylase[1]
    体外実験

    DMOG efficiently suppresses hydroxyproline synthesis in intact cells, but shows only weakly active in the microsomal system[1].?DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DMOG 関連抗体
    体内実験

    DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice[2]. Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    175.14

    分子式

    C6H9NO5
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(OC)CNC(C(OC)=O)=O
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 125 mg/mL (713.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 50 mg/mL (285.49 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.7097 mL 28.5486 mL 57.0972 mL
    5 mM 1.1419 mL 5.7097 mL 11.4194 mL
    10 mM 0.5710 mL 2.8549 mL 5.7097 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    一般には略語で表示されます：C1V1 = C2V2

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    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 150 mg/mL (856.46 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.93%

    COA (244 KB) HNMR (132 KB) RP-HPLC (255 KB) MS (72 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [3]

    To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    動物実験
    [2]

    Two groups of mice (C57Bl6) are used. One group (n=11) receives dimethyloxalylglycine (DMOG) i.p. (8 mg in 0.5 mL saline) on days 1, 3, 5, 7 and 9 while the animals in the other group are injected with sterile saline (0.5 mL) at the same intervals (n=6). A third group is treated with DMOG without ligation (n=4) and four unoperated mice serve as controls. After 11 days mice are terminally anaesthetized and the extensor digitorum longus (EDL) and tibialis anterior (TA) muscles excised.

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 5.7097 mL 28.5486 mL 57.0972 mL 142.7429 mL
    5 mM 1.1419 mL 5.7097 mL 11.4194 mL 28.5486 mL
    10 mM 0.5710 mL 2.8549 mL 5.7097 mL 14.2743 mL
    15 mM 0.3806 mL 1.9032 mL 3.8065 mL 9.5162 mL
    20 mM 0.2855 mL 1.4274 mL 2.8549 mL 7.1371 mL
    25 mM 0.2284 mL 1.1419 mL 2.2839 mL 5.7097 mL
    30 mM 0.1903 mL 0.9516 mL 1.9032 mL 4.7581 mL
    40 mM 0.1427 mL 0.7137 mL 1.4274 mL 3.5686 mL
    50 mM 0.1142 mL 0.5710 mL 1.1419 mL 2.8549 mL
    60 mM 0.0952 mL 0.4758 mL 0.9516 mL 2.3790 mL
    80 mM 0.0714 mL 0.3569 mL 0.7137 mL 1.7843 mL
    100 mM 0.0571 mL 0.2855 mL 0.5710 mL 1.4274 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    DMOG Related Classifications

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    • Dilution Calculator

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DMOG89464-63-1Dimethyloxallyl GlycineHIF/HIF Prolyl-HydroxylaseAutophagyHypoxia-inducible factorsHIFsHIF-PHdiarrhoeastabilisationaccmulationpro-angiogenicInhibitorinhibitorinhibit

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