DMOG (GMP) is the GMP level of DMOG (HY-15893). GMP small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. DMOG (GMP) is a HIF-1α stabilizer. DMOG (GMP) promotes the osteogenic, angiogenic, and chondrogenic differentiation of stem cells by stabilizing the expression of HIF-1α. DMOG (GMP) can enhance the osteogenic and angiogenic differentiation potential of stem cells, thereby improving bone regeneration in bone defects. DMOG (GMP) can be used in the research of bone defect repair, vascularized bone regeneration, and the treatment of bone-related diseases (such as osteoporosis and femoral head necrosis) .
For research use only. We do not sell to patients.
- CAS No.: 89464-63-1
- Formula: C6H9NO5
- Molecular Weight:175.14
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-15893
DMOGHIF-PH Inhibitor -
HY-15893R
DMOG (Standard)HIF-PH Inhibitor -
HY-13426
RoxadustatHIF-PH Inhibitor -
HY-15836
BAY 87-2243HIF-1 Inhibitor -
HY-10231
PX-478HIF-1α Inhibitor -
HY-13671
LW6Apoptosis Inducer -
HY-N0055
Chlorogenic acidAntioxidant -
HY-15127
IsotretinoinAnti-acne Agent -
HY-12754
ML228HIF Activator -
HY-15468
IOX2HIF/HIF Prolyl-Hydroxylase Inhibitor -
HY-15641
FG-2216HIF-PHD2 Inhibitor -
HY-12867
PT-2385HIF-2α Inhibitor
Biological Activity
Description
In Vitro
DMOG efficiently suppresses hydroxyproline synthesis in intact cells, but shows only weakly active in the microsomal system[1]. DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 89464-63-1
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Molecular Weight 175.14
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Formula C6H9NO5
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SMILES
O=C(OC)CNC(C(OC)=O)=O
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Synonyms
Dimethyloxallyl Glycine (GMP)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Mesenchymal stromal/stem cell chondrogenic differentiation
MSC chondrogenic differentiation is commonly induced by culturing bone marrow-derived mesenchymal stromal/stem cells as high-density three-dimensional pellets or micromass aggregates in defined chondrogenic medium containing TGF-β family stimulation; the readout is formation of cartilage-like extracellular matrix, especially sulfated proteoglycans, aggrecan, and type II collagen. The assay detects chondrogenesis by pellet enlargement, metachromatic or Alcian blue/Safranin O staining of proteoglycan-rich matrix, immunodetection of type II collagen and aggrecan, and gene-expression changes in cartilage matrix markers; hypertrophic or fibrocartilaginous drift can be assessed by collagen X and collagen I readouts when included.
Purity & Documentation
References
[1]. Singh A, et al. Hypoxia-inducible factor (HIF) prolyl hydroxylase inhibitors induce autophagy and have a protective effect in an in-vitro ischaemia model.Sci Rep. 2020 Jan 31;10(1):1597. [Content Brief]
[2]. Milkiewicz M, et al. Inhibition of endogenous HIF inactivation induces angiogenesis in ischaemic skeletal muscles of mice. J Physiol. 2004 Oct 1;560(Pt 1):21-6. [Content Brief]
[3]. Baader E, et al. Inhibition of prolyl 4-hydroxylase by oxalyl amino acid derivatives in vitro, in isolated microsomes and in embryonic chicken tissues. Biochem J. 1994 Jun 1;300 (Pt 2):525-30. [Content Brief]
[4]. Li X, et al. Up-regulation of hypoxia-inducible factor-1α enhanced the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats. Acta Biochim Biophys Sin (Shanghai). 2014 Feb;46(2):112-8. [Content Brief]
[5]. Schultz K, et al. Prolyl hydroxylase 2 deficiency limits proliferation of vascular smooth muscle cells by hypoxia-inducible factor-1{alpha}-dependent mechanisms. Am J Physiol Lung Cell Mol Physiol. 2009 Jun;296(6):L921-7. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DMOG (GMP) Related Classifications
HY-15893G Related Classifications
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