1. Membrane Transporter/Ion Channel
  2. Na+/K+ ATPase

Digoxin (Synonyms: 12β-Hydroxydigitoxin)

Cat. No.: HY-B1049 Purity: 98.46%
Handling Instructions

Digoxin is a potent inhibitor of Na+/K+-ATPase, clinically used to treat arrhythmia and heart failure.

For research use only. We do not sell to patients.

Digoxin Chemical Structure

Digoxin Chemical Structure

CAS No. : 20830-75-5

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
100 mg USD 60 In-stock
Estimated Time of Arrival: December 31
500 mg USD 120 In-stock
Estimated Time of Arrival: December 31
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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References


Digoxin is a potent inhibitor of Na+/K+-ATPase, clinically used to treat arrhythmia and heart failure.

IC50 & Target

Na+/K+ ATPase[1]

In Vitro

Digoxin, a sodium-potassium ATPase inhibitor, has a negative Z score of −26.67, suggesting a function for the sodium-potassium ATPase in CHIKV infection. Relative to DMSO-treated cells, treatment of U-2 OS cells with Digoxin results in a dose-dependent decrease in CHIKV infection with a half-maximal effective concentration (EC50) of 48.8 nM. Digoxin treatment similarly decreases CHIKV infection of primary human synovial fibroblasts (HSFs) and Vero African green monkey kidney cells with EC50s of 43.9 nM and 67.3 nM, respectively. Digoxin treatment significantly diminishes CHIKV infection in these cell types, with EC50s of 16.2 µM in ST2 cells and 23.2 µM in C2C12 cells, values 330 and 475 times the EC50 of Digoxin in U-2 OS cells. Cell viability is only modestly impaired at 24 h posttreatment with 1 µM Digoxin, a dose 20 times the Digoxin EC50 for CHIKV antiviral activity in these cells[1].

Clinical Trial
Solvent & Solubility
In Vitro: 

DMSO : ≥ 28 mg/mL (35.85 mM)

*"≥" means soluble, but saturation unknown.

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2805 mL 6.4025 mL 12.8051 mL
5 mM 0.2561 mL 1.2805 mL 2.5610 mL
10 mM 0.1281 mL 0.6403 mL 1.2805 mL
*Please refer to the solubility information to select the appropriate solvent.
Cell Assay

U-2 OS cells seeded in 60-mm-diameter dishes are incubated with DMSO, 10 µM STS as an inducer of apoptosis, or increasing concentrations of Digoxin at 37°C for 6 h. Cells are washed with fluorescence-activated cell sorter (FACS) buffer (PBS with 2% FBS) and stained with PI. Cell staining is quantified using a BD LSRII flow cytometer and FlowJo software. Alternatively, U-2 OS cells seeded in 96-well plates are incubated with DMSO, STS, or increasing concentrations of Digoxin at 37°C for 6 or 24 h. PrestoBlue reagent is added to supernatants of compound-treated cells, and cells are incubated at 37°C for 30 min. Fluorescence as a surrogate for cell viability is quantified using a Synergy H1 plate reader[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight







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4°C, protect from light


Room temperature in continental US; may vary elsewhere

Purity: 98.46%

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Cat. No.: HY-B1049