2. Anti-infection Immunology/Inflammation Autophagy
  3. Parasite Toll-like Receptor (TLR) SARS-CoV Autophagy
  4. Hydroxychloroquine sulfate

Hydroxychloroquine sulfate  (Synonyms: HCQ sulfate)

Cat. No.: HY-B1370 Purity: 99.95%
COA
SDS
Handling Instructions Technical Support

Hydroxychloroquine sulfate (HCQ sulfate) is a synthetic antimalarial agent which can also inhibit Toll-like receptor 7/9 (TLR7/9) signaling. Hydroxychloroquine sulfate is efficiently inhibits SARS-CoV-2 infection in vitro.

For research use only. We do not sell to patients.

CAS No. : 747-36-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in Water In-stock
Solid
50 mg In-stock
100 mg In-stock
200 mg In-stock
500 mg In-stock
1 g In-stock
5 g In-stock
10 g In-stock
50 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 87 publication(s) in Google Scholar

Other Forms of Hydroxychloroquine sulfate:

Hydroxychloroquine sulfate Related Antibodies

Top Publications Citing Use of Products

87 Publications Citing Use of MCE Hydroxychloroquine sulfate

Bio/Physico-chemical Assay
Cell Proliferation/Viability Assay
Cell Imaging/Staining
WB
In Vivo Efficacy Study

    Hydroxychloroquine sulfate purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2024 Oct 31;23(1):243.  [Abstract]

    The combenefit analysis was performed to identify the synergistic interaction of drug combinations (cDDP + venetoclax, cDDP + HCQ (50-100 μM, 72 h), or cDDP + venetoclax + HCQ (0-50 μM, 72 h)) in H446/cDDP or H196/cDDP cells.

    Hydroxychloroquine sulfate purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2024 Oct 31;23(1):243.  [Abstract]

    CCK-8 assay was used to detect the relative cell viability of H446/cDDP under drug combinations (cDDP + venetoclax, cDDP + HCQ (0-50 μM, 0-72 h), or cDDP + venetoclax + HCQ (0-50 μM, 0-72 h)) indicated above.

    Hydroxychloroquine sulfate purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2024 Oct 31;23(1):243.  [Abstract]

    The colony formation assay demonstrated and analyzed the effectiveness of HCQ (0-50 μM) within 14 days.

    Hydroxychloroquine sulfate purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2024 Oct 31;23(1):243.  [Abstract]

    Western blot analysis showed the expression levels of p62, LC3 I, and LC3 II in H446/cDDP cells after HCQ (0-50 μM, 0-72 h) treatment.

    Hydroxychloroquine sulfate purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2024 Oct 31;23(1):243.  [Abstract]

    Combinations of cDDP/VP-16/PTX, venetoclax and HCQ (15 mg/kg, once every two days, ip, 2-3 weeks) shrink the tumor volume of chemoresistant SCLC in PDX mice model.

    Hydroxychloroquine sulfate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Jun 14;13(1):3419.  [Abstract]

    Following treatment with the autophagy inhibitor hydroxychloroquine (HCQ, 60 mg/kg, once every other day, ip,18 days), Ursofalk no longer enhanced PD-1 antibody-mediated LLC tumor suppression.

    Hydroxychloroquine sulfate purchased from MedChemExpress. Usage Cited in: Autophagy. 2023 May;19(5):1533-1550.  [Abstract]

    293T cells were transfected with the indicated plasmids above for 8 h and then either treated with Hydroxychloroquine sulfate (CQ; 20 μM) or not. 12 h later, cell lysates were analyzed with western blotting.

    Hydroxychloroquine sulfate purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2021 Dec;11(12):3966-3982.  [Abstract]

    Representative photos of SH-SY5Y xenografted tumors. Mice were intraperitoneally treated with saline (Ctrl), magnolol (35 mg/kg), Hydroxychloroquine sulfate (HCQ; 50 mg/kg), Ac220 (1.5 mg/kg), magnolol þ HCQ, magnolol þ Ac220 for 23 days.

    View All Parasite Isoform Specific Products:

    View All Isoforms
    Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

    View All Toll-like Receptor (TLR) Isoform Specific Products:

    View All Isoforms
    TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Hydroxychloroquine sulfate (HCQ sulfate) is a synthetic antimalarial agent which can also inhibit Toll-like receptor 7/9 (TLR7/9) signaling. Hydroxychloroquine sulfate is efficiently inhibits SARS-CoV-2 infection in vitro[1][2][3].

    IC50 & Target

    TLR7

     

    Plasmodium

     

    TLR9

     

    Cellular Effect
    Cell Line Type Value Description References
    Vero C1008 CC50
    > 40 μM
    Compound: Hydroxychloroquine sulfate
    Cytotoxicity (CC50) determination in Vero E6 cells measured by fluorescence (OD590nm)
    Cytotoxicity (CC50) determination in Vero E6 cells measured by fluorescence (OD590nm)
    		10.1101/2020.04.03.023846
    Vero C1008 CC50
    >> 50 μM
    Compound: Hydroxychloroquine Sulfate
    CC50 determination at MOI 0.004 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    CC50 determination at MOI 0.004 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    		10.1101/2020.03.25.008482
    Vero C1008 CC50
    >> 50 μM
    Compound: Hydroxychloroquine Sulfate
    CC50 determination at MOI 0.01 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    CC50 determination at MOI 0.01 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    		10.1101/2020.03.25.008482
    Vero C1008 EC50
    4.17 μM
    Compound: Hydroxychloroquine sulfate
    Antiviral efficacy against SARS-CoV-2 (strain BavPat1) in Vero E6 cells assessed by inhibition of viral RNA replication measured by RT-PCR after 2 days
    Antiviral efficacy against SARS-CoV-2 (strain BavPat1) in Vero E6 cells assessed by inhibition of viral RNA replication measured by RT-PCR after 2 days
    		10.1101/2020.04.03.023846
    Vero C1008 IC50
    11.17 μM
    Compound: Hydroxychloroquine Sulfate
    IC50 determination at MOI 0.01 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in SARS-CoV-2 infected Vero E6 cells
    IC50 determination at MOI 0.01 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in SARS-CoV-2 infected Vero E6 cells
    		10.1101/2020.03.25.008482
    Vero C1008 IC50
    9.21 μM
    Compound: Hydroxychloroquine Sulfate
    IC50 determination at MOI 0.004 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in SARS-CoV-2 infected Vero E6 cells
    IC50 determination at MOI 0.004 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in SARS-CoV-2 infected Vero E6 cells
    		10.1101/2020.03.25.008482
    In Vitro

    Hydroxychloroquine sulfate is a synthetic antimalarial drug derived from 4-aminoquinoline; it has been used for several decades for the treatment of some rheumatic diseases such as rheumatoid arthritis (RA)[1]. Five micromolar Hydroxychloroquine sulfate or chloroquine also has no measurable effect on intracellular pH, although these concentrations can inhibit TLR9 or 7 signaling induced by DNA or RNA ligands[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Hydroxychloroquine sulfate Related Antibodies
    In Vivo

    Hydroxychloroquine sulfate is prescribed for the treatment of lupus, and both Hydroxychloroquine sulfate and its analog chloroquine inhibit TLR7 and 9 signaling[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    433.95

    Formula

    C18H28ClN3O5S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CCN(CCO)CCCC(NC1=CC=NC2=CC(Cl)=CC=C12)C.O=S(O)(O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    H2O : 125 mg/mL (288.05 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.3044 mL 11.5221 mL 23.0441 mL
    5 mM 0.4609 mL 2.3044 mL 4.6088 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (230.44 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.99%

    SDS (396 KB)

    COA (246 KB) HNMR (188 KB) LCMS (404 KB)

    Handling Instructions (2659 KB)
    References
    Animal Administration
    [2]

    MRL/lpr mice are dosed orally five times a week with 20 or 60 mg/kg E6446 or 60 mg/kg Hydroxychloroquine sulfate beginning at 5 weeks of age. CB 4564 is administered at 50 mg/kg i.p. every 10 days. A serum sample is taken immediately before the beginning of treatment to monitor changes in autoreactive antibodies. Subsequently, serum samples are collected approximately monthly and analyzed for anti-dsDNA by ELISA after 1:500 dilution. Body weights and urine samples are taken at the same interval, and proteinuria is assessed. Anti-nuclear antibodies (ANA) are assessed using commercially available HEp2 slide kits, with serum diluted to 1:100 in kit buffer. ANA scores are read blinded[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 2.3044 mL 11.5221 mL 23.0441 mL 57.6103 mL
    5 mM 0.4609 mL 2.3044 mL 4.6088 mL 11.5221 mL
    10 mM 0.2304 mL 1.1522 mL 2.3044 mL 5.7610 mL
    15 mM 0.1536 mL 0.7681 mL 1.5363 mL 3.8407 mL
    20 mM 0.1152 mL 0.5761 mL 1.1522 mL 2.8805 mL
    25 mM 0.0922 mL 0.4609 mL 0.9218 mL 2.3044 mL
    30 mM 0.0768 mL 0.3841 mL 0.7681 mL 1.9203 mL
    40 mM 0.0576 mL 0.2881 mL 0.5761 mL 1.4403 mL
    50 mM 0.0461 mL 0.2304 mL 0.4609 mL 1.1522 mL
    60 mM 0.0384 mL 0.1920 mL 0.3841 mL 0.9602 mL
    80 mM 0.0288 mL 0.1440 mL 0.2881 mL 0.7201 mL
    100 mM 0.0230 mL 0.1152 mL 0.2304 mL 0.5761 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Hydroxychloroquine sulfate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Hydroxychloroquine747-36-4HCQParasiteToll-like Receptor (TLR)SARS-CoVAutophagySARS coronavirusInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Hydroxychloroquine sulfate
    Cat. No.:
    HY-B1370
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (396 KB) Français - FR (396 KB) Deutsch - DE (396 KB) Norwegian - NO (396 KB) Español - ES (396 KB) Swedish - SV (396 KB) Italian - IT (396 KB) Korean - KR (396 KB) Portuguese - PT (396 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.