1. Apoptosis
  2. Ferroptosis
  3. L-Buthionine-(S,R)-sulfoximine

L-Buthionine-(S,R)-sulfoximine (Synonyms: L-Buthionine sulfoximine; L-BSO)

Cat. No.: HY-106376A Purity: ≥98.0%
Handling Instructions

L-Buthionine-(S,R)-sulfoximine is a cell-permeable, potent, fast acting and irreversible inhibitor of g-glutamylcysteine synthetase and depletes cellular glutathione levels. The IC50 value of L-Buthionine-(S,R)-sulfoximine on melanoma, breast and ovarian tumor specimens are 1.9 μM, 8.6 μM, and 29 μM, respectively.

For research use only. We do not sell to patients.

L-Buthionine-(S,R)-sulfoximine Chemical Structure

L-Buthionine-(S,R)-sulfoximine Chemical Structure

CAS No. : 83730-53-4

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Solution
10 mM * 1 mL in Water USD 66 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
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Estimated Time of Arrival: December 31
Solid
50 mg USD 60 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

Other Forms of L-Buthionine-(S,R)-sulfoximine:

Top Publications Citing Use of Products

1 Publications Citing Use of MCE L-Buthionine-(S,R)-sulfoximine

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

L-Buthionine-(S,R)-sulfoximine is a cell-permeable, potent, fast acting and irreversible inhibitor of g-glutamylcysteine synthetase and depletes cellular glutathione levels. The IC50 value of L-Buthionine-(S,R)-sulfoximine on melanoma, breast and ovarian tumor specimens are 1.9 μM, 8.6 μM, and 29 μM, respectively.

IC50 & Target

γ-glutamylcysteine synthetase[1].

In Vitro

L-Buthionine-(S,R)-sulfoximine (BSO: 50 μM) treatment for 48 hr results in a 95% decrease in ZAZ and M14 melanoma cell line GSH levels, and a 60% decrease in GST enzyme activity. GST-π protein and mRNA levels are significantly reduced in both cell lines[1]. L-Buthionine-(S,R)-sulfoximine (BSO) induces oxidative stress in a cell by irreversibly inhibiting g-glutamylcysteine synthetase, an essential enzyme for the synthesis of glutathione (GSH)[2].
L-Buthionine-(S,R)-sulfoximine (BSO) induces ferroptosis in cancer cells[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

BSO causes an elevated frequency of DNA deletions during mouse development. BSO treatment reduced GSH concentration in mouse fetuses by 55% and 70% at 2 mM and 20 mM BSO doses, respectively, compared to untreated mice. Co-treatment with 2 mM BSO and 20 mM NAC depleted GSH to a similar extent as 2 mM BSO, consistent with the function of BSO to inhibit the g-GCS enzyme indispensable for GSH synthesis. Like GSH, cysteine levels dropped following BSO treatment[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

222.31

Formula

C₈H₁₈N₂O₃S

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (224.91 mM; Need ultrasonic)

DMSO : < 1 mg/mL (ultrasonic) (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4982 mL 22.4911 mL 44.9822 mL
5 mM 0.8996 mL 4.4982 mL 8.9964 mL
10 mM 0.4498 mL 2.2491 mL 4.4982 mL
*Please refer to the solubility information to select the appropriate solvent.
References

Purity: ≥98.0%

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L-Buthionine-(S,R)-sulfoximine
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HY-106376A
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