2. Apoptosis
  3. TNF Receptor
  4. N-Formyl-Met-Leu-Phe

N-Formyl-Met-Leu-Phe  (Synonyms: fMLP; N-Formyl-MLF)

Cat. No.: HY-P0224 Pureté: 99.90%
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N-Formyl-Met-Leu-Phe (fMLP; N-Formyl-MLF) is a chemotactic peptide and a specific ligand of N-formyl peptide receptor (FPR). N-Formyl-Met-Leu-Ph is reported to inhibit TNF-alpha secretion.

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Synthèse de peptides personnalisée

CAS No. : 59880-97-6

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Based on 16 publication(s) in Google Scholar

N-Formyl-Met-Leu-Phe Related Antibodies

Top Publications Citing Use of Products

    N-Formyl-Met-Leu-Phe purchased from MedChemExpress. Usage Cited in: ACS Nano. 2024 Feb 9.  [Abstract]

    fMLP (10 nM; 8 h). Fluorescence signals in the lower chamber with or without TNF-α and fMLP (Tf) were detected at designed intervals.

    N-Formyl-Met-Leu-Phe purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2022 May;19(5):602-618.  [Abstract]

    BMDMs from WT mice were pretreated with fMLP (10 μM) for 1 hour, then stimulated with heat-killed C. albicans (MOI=2) for 3 hours, followed by real-time PCR analysis of indicated gene expression.

    N-Formyl-Met-Leu-Phe purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2022 May;19(5):602-618.  [Abstract]

    BMDMs from WT mice were pretreated with fMLP (10 μM) for 1 hour, then stimulated with heat-killed C. albicans (MOI=2) for the indicated times, followed by immunoblot analysis of indicated protein expression.

    N-Formyl-Met-Leu-Phe purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2022 May;19(5):602-618.  [Abstract]

    fMLP (0.5 μg/g/50 μL; ip). WT mice were intravenous injected with live C. albicans, and were injected with DMSO or fMLP 12 hours after fungal injection. Observe the weight loss of the mice.

    N-Formyl-Met-Leu-Phe purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2022 May;19(5):602-618.  [Abstract]

    fMLP (0.5 μg/g/50 μL; ip). WT mice were intravenous injected with live C. albicans, and were injected with DMSO or fMLP 12 hours after fungal injection. Cells infiltrated in the kidneys were analyzed by flow cytometry.

    Voir tous les produits spécifiques à Isoform TNF Receptor:

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    TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF10B/DR5/CD262 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

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    Description

    N-Formyl-Met-Leu-Phe (fMLP; N-Formyl-MLF) is a chemotactic peptide and a specific ligand of N-formyl peptide receptor (FPR). N-Formyl-Met-Leu-Ph is reported to inhibit TNF-alpha secretion.

    IC50 & Target

    TNF-alpha[1]
    Cellular Effect
    Cell Line Type Value Description References
    HL-60 EC50
    0.01 μM
    Compound: fMLF
    Agonist activity at FPR1 expressed in human HL60 cells assessed as induction of intracellular calcium flux by FLIPR3 calcium assay
    Agonist activity at FPR1 expressed in human HL60 cells assessed as induction of intracellular calcium flux by FLIPR3 calcium assay
    		[PMID: 19639995]
    HL-60 EC50
    1.9 μM
    Compound: fMLF
    Agonist activity at FPRL2 expressed in human HL60 cells assessed as induction of intracellular calcium flux by FLIPR3 calcium assay
    Agonist activity at FPRL2 expressed in human HL60 cells assessed as induction of intracellular calcium flux by FLIPR3 calcium assay
    		[PMID: 19639995]
    HL-60 EC50
    20.4 μM
    Compound: fMLF
    Agonist activity at FPRL1 expressed in human HL60 cells assessed as induction of intracellular calcium flux by FLIPR3 calcium assay
    Agonist activity at FPRL1 expressed in human HL60 cells assessed as induction of intracellular calcium flux by FLIPR3 calcium assay
    		[PMID: 19639995]
    In Vitro

    Binding of N-Formyl-Met-Leu-Phe to its specific cell surface receptor, N-formyl peptide receptor (FPR), triggers different cascades of biochemical events, eventually leading to cellular activation. FPR is a chemoattractant receptor belonging to the G protein-coupled receptor family. N-Formyl-Met-Leu-Phe promotes osteoblastic commitment and suppresses adipogenic commitment under osteoblastic differentiation conditions. N-Formyl-Met-Leu-Phe stimulates osteogenesis is associated with increased expression of osteogenic markers and mineralization. N-Formyl-Met-Leu-Phe inhibits expression of peroxisome proliferator-activated receptor-γ1. N-Formyl-Met-Leu-Phe-stimulated osteogenic differentiation is mediated via FPR1-phospholipase C/phospholipase D-Ca2+-calmodulin-dependent kinase II-ERK-CREB signaling pathways[1]. N-Formyl-Met-Leu-Phe, a bacterial-derived peptide, induced proinflammatory cytokine gene expression in human peripheral blood monocytes. Bacterial products LPS and N-Formyl-Met-Leu-Phe synergistically induce inflammatory response via multiple signaling pathways. TLR4, IKKβ-IκBα, and NF-κB signaling pathways are involved in the synergistic induction of TNF-α via p65 nuclear translocation-dependent mechanisms[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    N-Formyl-Met-Leu-Phe Related Antibodies
    In Vivo

    N-Formyl-Met-Leu-Phe promotes bone formation in zebrafish and rabbits. Extensive skeletal development is evident at 5 dpf in over 80% of N-Formyl-Met-Leu-Phe-treated zebrafish. Treatment with N-Formyl-Met-Leu-Phe results in increased expression of Runx2. Bone marrow spaces are widely formed, and connective tissue covering bone is dense, like periosteum, in N-Formyl-Met-Leu-Phe-treated calvaria[1]. N-Formyl-Met-Leu-Phe mediate release of calprotectin from PMN in vitro. It induces release of calprotectin from PMN in a dose dependent manner. A minimum of 10% of total PMN calprotectin is retained at concentrations of 0.1-10.0 nM of N-Formyl-Met-Leu-Phe[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Masse moléculaire

    437.55

    Formule

    C21H31N3O5S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    Formyl-Met-Leu-Phe
    Sequence Shortening

    Formyl-MLF
    Livraison

    Room temperature in continental US; may vary elsewhere.
    Stockage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvant et solubilité
    In Vitro: 

    DMSO : 133.33 mg/mL (304.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2855 mL 11.4273 mL 22.8545 mL
    5 mM 0.4571 mL 2.2855 mL 4.5709 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Pureté et documentation

    Purity: 99.90%

    SDS (394 KB)

    COA (249 KB) RP-HPLC (249 KB) MS (213 KB) Elemental Analysis Report (109 KB)

    Instruction de manipulation (2659 KB)
    Références
    Test cellulaire
    [2]

    Cells are cotransfected with either a dominant negative form of IκBα or a dominant negative form of IKKβ together with the NF-κB-dependent luciferase reporter plasmid. The plasmid pCMVβ is used as a control for transfection efficiency and this is monitored via the expression of β-galactosidase. Cells are transiently transfected with plasmids using DEAE-dextran. The transfected cells are cultivated for 48 h before a 6-h incubation in medium ±N-Formyl-Met-Leu-Phe, LPS, or N-Formyl-Met-Leu-Phe/LPS. Luciferase activity is determined by using the luciferase assay kit and a Monolight 3010 luminometer[2].

    MCE n'a pas confirmé de manière indépendante l'exactitude de ces méthodes. Ils sont pour référence seulement.
    Administration animale
    [2]

    Mice: N-Formyl-Met-Leu-Phe is prepared in sterile PBS. Under the anesthesia, mice are intranasally treated with LPS (0.3 mg/kg) or N-Formyl-Met-Leu-Phe (0.5 mg/kg) or N-Formyl-Met-Leu-Phe and LPS in 50 μL of sterile PBS (control), BAL is performed by cannulating the trachea with sterilized PBS, and cells from BAL fluid are stained with Wright-Giemsa stain after cytocentrifuge. For TNF-α protein release, BAL fluid is collected and secreted TNF-α is measured by ELISA as described above[2].

    MCE n'a pas confirmé de manière indépendante l'exactitude de ces méthodes. Ils sont pour référence seulement.
    Références

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.2855 mL 11.4273 mL 22.8545 mL 57.1363 mL
    5 mM 0.4571 mL 2.2855 mL 4.5709 mL 11.4273 mL
    10 mM 0.2285 mL 1.1427 mL 2.2855 mL 5.7136 mL
    15 mM 0.1524 mL 0.7618 mL 1.5236 mL 3.8091 mL
    20 mM 0.1143 mL 0.5714 mL 1.1427 mL 2.8568 mL
    25 mM 0.0914 mL 0.4571 mL 0.9142 mL 2.2855 mL
    30 mM 0.0762 mL 0.3809 mL 0.7618 mL 1.9045 mL
    40 mM 0.0571 mL 0.2857 mL 0.5714 mL 1.4284 mL
    50 mM 0.0457 mL 0.2285 mL 0.4571 mL 1.1427 mL
    60 mM 0.0381 mL 0.1905 mL 0.3809 mL 0.9523 mL
    80 mM 0.0286 mL 0.1428 mL 0.2857 mL 0.7142 mL
    100 mM 0.0229 mL 0.1143 mL 0.2285 mL 0.5714 mL
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    N-Formyl-Met-Leu-Phe Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    N-Formyl-Met-Leu-Phe59880-97-6fMLP N-Formyl-MLFTNF ReceptorTumor Necrosis Factor ReceptorTNFRInhibitorinhibitorinhibit

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