1. Neuronal Signaling
    Metabolic Enzyme/Protease
    Apoptosis
  2. Monoamine Oxidase
    COMT
    Apoptosis
  3. Rosmarinic acid

Rosmarinic acid (Synonyms: Labiatenic acid)

Cat. No.: HY-N0529 Purity: 99.29%
Handling Instructions

Rosmarinic acid is a widespread phenolic ester compound in the plants. Rosmarinic acid inhibits MAO-A, MAO-B and COMT enzymes with IC50s of 50.1, 184.6 and 26.7 μM, respectively.

For research use only. We do not sell to patients.

Rosmarinic acid Chemical Structure

Rosmarinic acid Chemical Structure

CAS No. : 20283-92-5

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10 mM * 1 mL in DMSO USD 73 In-stock
Estimated Time of Arrival: December 31
50 mg USD 66 In-stock
Estimated Time of Arrival: December 31
100 mg USD 108 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Rosmarinic acid is a widespread phenolic ester compound in the plants. Rosmarinic acid inhibits MAO-A, MAO-B and COMT enzymes with IC50s of 50.1, 184.6 and 26.7 μM, respectively.

IC50 & Target

IC50: 50.1 μM (MAO-A), 184.6 μM (MAO-B), 26.7 μM (COMT)[1]

In Vitro

Rosmarinic acid (RA) shows an in vitro multifunctional profile characterized by antioxidant effects, and monoamine oxidases (MAO-A and MAO-B) and catechol-O-methyl transferase (COMT) inhibition. Rosmarinic acid shows antioxidant effects against hydroxyl (HO(•)) and nitric oxide (NO) radicals (IC50 of 29.4 and 140 μM, respectively), and inhibition of lipid peroxidation (IC50 of 19.6 μM)[1]. Rosmarinic acid (RA) exerts a significant cytoprotective effect by scavenging intracellular ROS induced by UVB. In H2O2-treated cells, 2.5 μM Rosmarinic acid scavenges 60% of intracellular ROS compared to 77% of intracellular ROS scavenging effect in N-acetyl-L-cysteine (NAC)[2].

In Vivo

Rosmarinic acid (RA) is a widespread phenolic ester compound in the plants, particularly those in the Labiatae family of herbs, such as Rosmarinus officinali, Salvia miltiorrhiza, and Prunella vulgaris. Rosmarinic acid suppresses colonic inflammation in dextran sulphate sodium (DSS)-induced mice via dual inhibition of NF-κB and STAT3 activation. In the DSS-induced colitis model, Treatment with Rosmarinic acid (30, 60 mg/kg, p.o.) markedly attenuates the production of cytokines[3].

Clinical Trial
Molecular Weight

360.31

Formula

C₁₈H₁₆O₈

CAS No.

20283-92-5

SMILES

OC([[email protected]](OC(/C=C/C1=CC(O)=C(O)C=C1)=O)CC2=CC(O)=C(O)C=C2)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (277.54 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7754 mL 13.8769 mL 27.7539 mL
5 mM 0.5551 mL 2.7754 mL 5.5508 mL
10 mM 0.2775 mL 1.3877 mL 2.7754 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (6.94 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (6.94 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.94 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[2]

Human keratinocytes (HaCaT cells) are treated with Rosmarinic acid (0.625, 1.25, 2.5, or 5 μM) and exposed to UVB radiation 1 h later. They are then incubated at 37°C for 48 h. At this time, MTT is added to each well to obtain a total reaction volume of 200 μL. After 4 h incubation, the supernatant is removed by aspiration. The formazan crystals in each well are dissolved in dimethyl sulfoxide (DMSO; 150 μL), and the absorbance at 540 nm is measured on a scanning multi-well spectrophotometer[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[3]

Mice[3]
Experimental colitis is induced by giving mice drinking water ad libitum containing 5% (w/v) DSS for 7 days. Mice of each of the groups are monitored carefully every day to confirm that they have consumed an approximately equal volume of water containing DSS. For each experiment, the mice are divided into five experimental groups (n = 10/group). The first group is kept as the vehicle-treated control, and the second group is given drinking water with DSS only during the experimental period. The other three groups consist of mice receiving 5% DSS who are administrated 5-ASA (75 mg/kg/day p.o.) or Rosmarinic acid (30 or 60 mg/kg/day p.o.) daily for 7 days. Control groups are given the vehicle daily for 7 days as appropriate. Administration of each drug is initiated simultaneously with the DSS treatment.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.29%

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Keywords:

Rosmarinic acidLabiatenic acidMonoamine OxidaseCOMTApoptosisMAOCatechol-O-methyltransferaseInhibitorinhibitorinhibit

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Rosmarinic acid
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