Sinomenine hydrochloride
Based on 23 publication(s) in Google Scholar
Sinomenine hydrochloride (Cucoline hydrochloride), an alkaloid extracted from Sinomenium acutum, is a blocker of the NF-κB activation. Sinomenine also is an activator of μ-opioid receptor.
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- Pureté: 99.89%
- CAS No.: 6080-33-7
- Formule: C19H24ClNO4
- Masse moléculaire:365.85
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Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
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Publications Citing Use of MedChemExpress (MCE) Sinomenine hydrochloride
More- Acta Pharm Sin B. 2021 Nov;11(11):3465-3480. [Abstract]
- Phytomedicine. 2025 May:140:156484. [Abstract]
- Phytother Res. 2023 Aug;37(8):3323-3341. [Abstract]
- Free Radic Biol Med. 2018 Aug 20:124:205-213. [Abstract]
- Anal Chem. 2025 Dec 19. [Abstract]
- PLoS Biol. 2024 Jun 27;22(6):e3002672. [Abstract]
- Life Sci. 2026 Mar 1:388:124199. [Abstract]
- Drug Des Devel Ther. 2024 Aug 6:18:3523-3545. [Abstract]
- Eur J Pharmacol. 2026 Jun 11:1030:178976. [Abstract]
- Eur J Pharmacol. 2026 May 10:1023:178881. [Abstract]
- Int Immunopharmacol. 2026 Oct 1:186:117153.
- Arab J Chem. 2026:19;14522025.
- World J Stem Cells. 2024 May 26;16(5):486-498. [Abstract]
- J Dairy Sci. 2025 Nov 7:S0022-0302(25)00911-7. [Abstract]
- J Inflamm Res. 2023 Oct 20:16:4777-4791. [Abstract]
- FASEB J. 2026 Jul 15;40(13):e72090.
- Naunyn Schmiedebergs Arch Pharmacol. 2025 May 17. [Abstract]
- Immun Inflamm Dis. 2024 Jun;12(6):e1271. [Abstract]
- Chem Biol Drug Des. 2023 Sep;102(3):434-443. [Abstract]
- Tissue Cell. 2026 Jul 9;104(Pt 1):103766.
- Exp Ther Med. 2021 Jun;21(6):647. [Abstract]
- Heliyon. 2024 Jun 24;10(13):e33314. [Abstract]
- bioRxiv. 2024 Apr 3:2023.06.02.542933. [Abstract]
Customer Validation & Images
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Cell Proliferation/Viability Assay
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Flow Cytometry
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RT-PCR
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WB
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Histological Imaging/Staining
Voir tous les produits spécifiques à Isoform Opioid Receptor
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Activité biologique
Description
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NF-κB |
μ Opioid Receptor/MOR |
In Vitro
Cell viability is gradually decreased with increasing Sinomenine hydrochloride concentration. The migration ability of MDA-MB-231 cells is significantly weakened by 0.25, 0.5, and 1 mM of Sinomenine hydrochloride treatment. The wound-healing assay reveals that 0.25 and 0.5 mM Sinomenine hydrochloride significantly suppress the healing of the wound. When the MDA-MB-231 cells are treated with 0.5 mM Sinomenine hydrochloride, the healing progress is about 50%, but in the group treated with 0.25 mM Sinomenine hydrochloride and the untreated control, the healing is about 80% and nearly 95%, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Essai clinique
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 6080-33-7
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Appearance Solid
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Masse moléculaire 365.85
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Formule C19H24ClNO4
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Color White to off-white
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SMILES
OC(C1=C2C[C@@]3([H])[C@](C=C4OC)([H])[C@@]1(CCN3C)CC4=O)=C(C=C2)OC.Cl
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Synonyms
Cucoline hydrochloride
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)
Publications (23)
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Journal Impact Factor
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Most Recent
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Acta Pharm Sin B
Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway. [Abstract]2021 Nov;11(11):3465-3480. PMID: 34900530 -
Phytomedicine
Fangchinoline suppresses nasopharyngeal carcinoma progression by inhibiting SQLE to regulate the PI3K/AKT pathway dysregulation. [Abstract]2025 May:140:156484. PMID: 40090046 -
Phytother Res
Cognitive protection of sinomenine in type 2 diabetes mellitus through regulating the EGF/Nrf2/HO-1 signaling, the microbiota-gut-brain axis, and hippocampal neuron ferroptosis. [Abstract]2023 Aug;37(8):3323-3341. PMID: 37036428 -
Free Radic Biol Med
Cold atmospheric plasma conveys selectivity on triple negative breast cancer cells both in vitro and in vivo. [Abstract]2018 Aug 20:124:205-213. PMID: 29870749
Sinomenine hydrochloride purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2018 Aug 20:124:205-213. [Abstract]
Total and phosphorylation levels of p65 in MCF7 and MDAMB231 after Sinomenine hydrochloride (SH) treatment at 24 h.
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Anal Chem
Noninvasive Dynamic Cell Viability Monitoring Based on Diffraction Height Fingerprint Spectra. [Abstract]2025 Dec 19. PMID: 41417584 -
PLoS Biol
2024 Jun 27;22(6):e3002672. PMID: 38935621 -
Life Sci
Sinomenine ameliorates myocardial ischemia/reperfusion injury by inhibiting mitochondrial oxidative stress-mediated PANoptosis and ferroptosis via α7nAChR. [Abstract]2026 Mar 1:388:124199. PMID: 41520699 -
Drug Des Devel Ther
Sinomenine Alleviates Rheumatoid Arthritis by Suppressing the PI3K-Akt Signaling Pathway, as Demonstrated Through Network Pharmacology, Molecular Docking, and Experimental Validation. [Abstract]2024 Aug 6:18:3523-3545. PMID: 39135759 -
Eur J Pharmacol
Sinomenine alleviates ulcerative colitis by targeting FXR to regulate arachidonic acid metabolism and Th17/Treg homeostasis. [Abstract]2026 Jun 11:1030:178976. PMID: 42269904 -
Eur J Pharmacol
Sinomenine modulates autoantigen-specific immune responses by inhibiting dendritic cell activation in experimental arthritis. [Abstract]2026 May 10:1023:178881. PMID: 42000020 -
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World J Stem Cells
Sinomenine increases osteogenesis in mice with ovariectomy-induced bone loss by modulating autophagy. [Abstract]2024 May 26;16(5):486-498. PMID: 38817333 -
J Dairy Sci
Sinomenine hydrochloride ameliorates fatty acid-induced bovine mammary epithelial cells' oxidative stress and inflammation via enhancing autophagy activity. [Abstract]2025 Nov 7:S0022-0302(25)00911-7. PMID: 41207443 -
J Inflamm Res
Sinomenine Ameliorates IL-1β-Induced Intervertebral Disc Degeneration in Rats Through Suppressing Inflammation and Oxidative Stress via Keap1/Nrf2/NF-κB Signaling Pathways. [Abstract]2023 Oct 20:16:4777-4791. PMID: 37881650
Sinomenine hydrochloride purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2023 Oct 20:16:4777-4791. [Abstract]
Effect of Sinomenine (SN) (1, 2, 4, 8, 16 μM) on the activity of NPCs.
Sinomenine hydrochloride purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2023 Oct 20:16:4777-4791. [Abstract]
Pretreatment with Sinomenine (SN) (4 μM) for 8 hours decreased ROS content and apoptosis.
Sinomenine hydrochloride purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2023 Oct 20:16:4777-4791. [Abstract]
Pre-treatment with Sinomenine (SN) (4 μM) for 8 hours reversed IL-1β induced the expression of DCN and COL2A1 by RT-PCR.
Sinomenine hydrochloride purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2023 Oct 20:16:4777-4791. [Abstract]
Pre-treatment with Sinomenine (SN) (4 μM) for 8 hours reversed IL-1β induced the expression of DCN and COL2A1 by Western Blot.
Sinomenine hydrochloride purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2023 Oct 20:16:4777-4791. [Abstract]
HE staining showed that Sinomenine (SN) improves IDD by suppressing the inflammatory response.
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Naunyn Schmiedebergs Arch Pharmacol
Sinomenine alleviates gouty inflammation by inhibiting macrophage M1 polarization and neutrophil extracellular trap formation. [Abstract]2025 May 17. PMID: 40381009 -
Immun Inflamm Dis
High-dose sinomenine attenuates ischemia/reperfusion-induced hepatic inflammation and oxidative stress in rats with diabetes mellitus. [Abstract]2024 Jun;12(6):e1271. PMID: 38888355 -
Chem Biol Drug Des
Sinomenine attenuates lipopolysaccharide-induced inflammation and apoptosis of WI-38 cells by reducing GSTM1 expression. [Abstract]2023 Sep;102(3):434-443. PMID: 36303295 -
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Exp Ther Med
Sinomenine activation of Nrf2 signaling prevents inflammation and cerebral injury in a mouse model of ischemic stroke. [Abstract]2021 Jun;21(6):647. PMID: 33968178 -
Heliyon
Sinomenine ameliorates bleomycin-induced pulmonary fibrosis by inhibiting the differentiation of fibroblast into myofibroblast. [Abstract]2024 Jun 24;10(13):e33314. PMID: 39050413 -
bioRxiv
An efficient behavioral screening platform classifies natural products and other chemical cues according to their chemosensory valence in C. elegans. [Abstract]2024 Apr 3:2023.06.02.542933. PMID: 37333363
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (273.34 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 16.67 mg/mL (45.57 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.69 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.69 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 25 mg/mL (68.33 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocole
Cell Assay[1]
The MDA-MB-231 human triple negative and 4T1 mouse breast cancer cell lines are used in this study. For the experiments, the cells are grown in 24-well plates at 3.5×104 cells/well. Following incubation for 24 or 48 h in medium containing different concentrations of Sinomenine hydrochloride, proliferation of the cells are detected with Cell Counting Kit-8 solution according to the manufacturer's instructions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration[4]
Male Sprague-Dawley rats weighing of 250 to 300 g are used in this experiment. For the duration of action of acute Sinomenine hydrochloride study, different doses of Sinomenine hydrochloride (10 to 40 mg/kg) are administered 1 day after surgery and then paw withdrawal threshold is measured every 30 min for 4 hours. For the study involving daily Sinomenine hydrochloride treatment, mechanical hyperalgesia measure is performed 3 h after daily drug treatment. For antagonist studies, antagonists are given 10 min prior to 40 mg/kg Sinomenine hydrochloride administration[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureté et documentation
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Fiche technique (284 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Song L, et al. Sinomenine inhibits breast cancer cell invasion and migration by suppressing NF-κB activation mediated by IL-4/miR-324-5p/CUEDC2 axis. Biochem Biophys Res Commun. 2015 Aug 28;464(3):705-10. [Content Brief]
[2]. Wang MH, et al. Activation of opioid mu-receptor by sinomenine in cell and mice.Neurosci Lett. 2008 Oct 10;443(3):209-12. [Content Brief]
[3]. Gao T, et al. Analgesic effect of sinomenine in rodents after inflammation and nerve injury. Eur J Pharmacol. 2013 Dec 5;721(1-3):5-11. [Content Brief]
[4]. Zhu Q, et al. Antinociceptive effects of sinomenine in a rat model of neuropathic pain. Sci Rep. 2014 Dec 1;4:7270. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.7334 mL | 13.6668 mL | 27.3336 mL | 68.3340 mL |
| 5 mM | 0.5467 mL | 2.7334 mL | 5.4667 mL | 13.6668 mL | |
| 10 mM | 0.2733 mL | 1.3667 mL | 2.7334 mL | 6.8334 mL | |
| 15 mM | 0.1822 mL | 0.9111 mL | 1.8222 mL | 4.5556 mL | |
| 20 mM | 0.1367 mL | 0.6833 mL | 1.3667 mL | 3.4167 mL | |
| 25 mM | 0.1093 mL | 0.5467 mL | 1.0933 mL | 2.7334 mL | |
| 30 mM | 0.0911 mL | 0.4556 mL | 0.9111 mL | 2.2778 mL | |
| 40 mM | 0.0683 mL | 0.3417 mL | 0.6833 mL | 1.7084 mL | |
| DMSO | 50 mM | 0.0547 mL | 0.2733 mL | 0.5467 mL | 1.3667 mL |
| 60 mM | 0.0456 mL | 0.2278 mL | 0.4556 mL | 1.1389 mL | |
| 80 mM | 0.0342 mL | 0.1708 mL | 0.3417 mL | 0.8542 mL | |
| 100 mM | 0.0273 mL | 0.1367 mL | 0.2733 mL | 0.6833 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Sinomenine hydrochloride Related Classifications
HY-15122A Related Classifications
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