1. NF-κB
    Neuronal Signaling
    GPCR/G Protein
    Autophagy
    Apoptosis
  2. NF-κB
    Opioid Receptor
    Autophagy
    Apoptosis
  3. Sinomenine hydrochloride

Sinomenine hydrochloride (Synonyms: Cucoline hydrochloride)

Cat. No.: HY-15122A Purity: 99.46%
Handling Instructions

Sinomenine hydrochloride (Cucoline hydrochloride), an alkaloid extracted from Sinomenium acutum, is a blocker of the NF-κB activation. Sinomenine also is an activator of μ-opioid receptor.

For research use only. We do not sell to patients.

Sinomenine hydrochloride Chemical Structure

Sinomenine hydrochloride Chemical Structure

CAS No. : 6080-33-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
100 mg USD 60 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Sinomenine hydrochloride:

Top Publications Citing Use of Products

Publications Citing Use of MCE Sinomenine hydrochloride

    Sinomenine hydrochloride purchased from MCE. Usage Cited in: Free Radic Biol Med. 2018 Jun 2;124:205-213.

    Total and phosphorylation levels of p65 in MCF7 and MDAMB231 after Sinomenine hydrochloride (SH) treatment at 24 h.

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    • Biological Activity

    • Protocol

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    • References

    • Customer Review

    Description

    Sinomenine hydrochloride (Cucoline hydrochloride), an alkaloid extracted from Sinomenium acutum, is a blocker of the NF-κB activation[1]. Sinomenine also is an activator of μ-opioid receptor[2].

    IC50 & Target[1][2]

    NF-κB

     

    μ-opioid receptor

     

    In Vitro

    Cell viability is gradually decreased with increasing Sinomenine hydrochloride concentration. The migration ability of MDA-MB-231 cells is significantly weakened by 0.25, 0.5, and 1 mM of Sinomenine hydrochloride treatment. The wound-healing assay reveals that 0.25 and 0.5 mM Sinomenine hydrochloride significantly suppress the healing of the wound. When the MDA-MB-231 cells are treated with 0.5 mM Sinomenine hydrochloride, the healing progress is about 50%, but in the group treated with 0.25 mM Sinomenine hydrochloride and the untreated control, the healing is about 80% and nearly 95%, respectively. The IB assay following inhibitor of NF-κB (IκB) antibody IP shows that the binding of NF-κB to IκB is inhibited by Sinomenine hydrochloride treatment in a dose-dependent manne[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Sinomenine hydrochloride (i.p.) produces antinociception in the hot plate and tail flick tests in male rats at 40 mg/kg, but not at lower doses (10 or 20 mg/kg). At 10 to 40 mg/kg Sinomenine hydrochloride does not produce any observable side effect such as sedation, allergy or motor impairments. At 80 mg/kg, Sinomenine hydrochloride is mildly sedative in rats. Sinomenine hydrochloride at 80 mg/kg i.p. does not produce any observable side effects in mice. I.p or p.o. Sinomenine hydrochloride at 40 or 80 mg/kg dose-dependently reduces mechanical hypersensitivity in nerve injured mice. I.p. Sinomenine hydrochloride at 40 mg/kg, but not lower doses or vehicle, significantly decreases mechanical and cold allodynia for up to 240 min without producing motor deficits or sedation[3]. At doses of 10 to 40 mg/kg, Sinomenine hydrochloride dose-dependently increases the paw withdrawal threshold. In non-chronic constriction injury (CCI) healthy rats, Sinomenine hydrochloride at the dose range of 10 to 40 mg/kg does not change the immobility behavior in the forced swimming test[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    365.85

    Formula

    C₁₉H₂₄ClNO₄

    CAS No.

    6080-33-7

    SMILES

    OC(C1=C2C[[email protected]@]3([H])[[email protected]](C=C4OC)([H])[[email protected]@]1(CCN3C)CC4=O)=C(C=C2)OC.Cl

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (273.34 mM; Need ultrasonic and warming)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7334 mL 13.6668 mL 27.3336 mL
    5 mM 0.5467 mL 2.7334 mL 5.4667 mL
    10 mM 0.2733 mL 1.3667 mL 2.7334 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (6.83 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (6.83 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (6.83 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Cell Assay
    [1]

    The MDA-MB-231 human triple negative and 4T1 mouse breast cancer cell lines are used in this study. For the experiments, the cells are grown in 24-well plates at 3.5×104 cells/well. Following incubation for 24 or 48 h in medium containing different concentrations of Sinomenine hydrochloride, proliferation of the cells are detected with Cell Counting Kit-8 solution according to the manufacturer's instructions[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [4]

    Male Sprague-Dawley rats weighing of 250 to 300 g are used in this experiment. For the duration of action of acute Sinomenine hydrochloride study, different doses of Sinomenine hydrochloride (10 to 40 mg/kg) are administered 1 day after surgery and then paw withdrawal threshold is measured every 30 min for 4 hours. For the study involving daily Sinomenine hydrochloride treatment, mechanical hyperalgesia measure is performed 3 h after daily drug treatment. For antagonist studies, antagonists are given 10 min prior to 40 mg/kg Sinomenine hydrochloride administration[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Keywords:

    SinomenineCucolineNF-κBOpioid ReceptorAutophagyApoptosisNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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    Product Name:
    Sinomenine hydrochloride
    Cat. No.:
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