1. NF-κB
    Neuronal Signaling
    GPCR/G Protein
  2. NF-κB
    Opioid Receptor
  3. Sinomenine hydrochloride

Sinomenine hydrochloride (Synonyms: Cucoline hydrochloride)

Cat. No.: HY-15122A Purity: >98.0%
Handling Instructions

Sinomenine hydrochloride is a blocker of the NF-κB activation and also an activator of μ-opioid receptor.

For research use only. We do not sell to patients.

Sinomenine hydrochloride Chemical Structure

Sinomenine hydrochloride Chemical Structure

CAS No. : 6080-33-7

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10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
100 mg USD 60 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Sinomenine hydrochloride purchased from MCE. Usage Cited in: Free Radic Biol Med. 2018 Jun 2;124:205-213.

    Total and phosphorylation levels of p65 in MCF7 and MDAMB231 after Sinomenine hydrochloride (SH) treatment at 24 h.

    View All NF-κB Isoform Specific Products:

    • Biological Activity

    • Protocol

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    Sinomenine hydrochloride is a blocker of the NF-κB activation and also an activator of μ-opioid receptor.

    IC50 & Target



    μ-opioid receptor


    In Vitro

    Cell viability is gradually decreased with increasing Sinomenine hydrochloride concentration. The migration ability of MDA-MB-231 cells is significantly weakened by 0.25, 0.5, and 1 mM of Sinomenine hydrochloride treatment. The wound-healing assay reveals that 0.25 and 0.5 mM Sinomenine hydrochloride significantly suppress the healing of the wound. When the MDA-MB-231 cells are treated with 0.5 mM Sinomenine hydrochloride, the healing progress is about 50%, but in the group treated with 0.25 mM Sinomenine hydrochloride and the untreated control, the healing is about 80% and nearly 95%, respectively. The IB assay following inhibitor of NF-κB (IκB) antibody IP shows that the binding of NF-κB to IκB is inhibited by Sinomenine hydrochloride treatment in a dose-dependent manne[1].

    In Vivo

    I.p. Sinomenine hydrochloride produces antinociception in the hot plate and tail flick tests in male rats at 40 mg/kg, but not at lower doses (10 or 20 mg/kg). At 10 to 40 mg/kg Sinomenine hydrochloride does not produce any observable side effect such as sedation, allergy or motor impairments. At 80 mg/kg, Sinomenine hydrochloride is mildly sedative in rats. Sinomenine hydrochloride at 80 mg/kg i.p. does not produce any observable side effects in mice. I.p or p.o. Sinomenine hydrochloride at 40 or 80 mg/kg dose-dependently reduces mechanical hypersensitivity in nerve injured mice. I.p. Sinomenine hydrochloride at 40 mg/kg, but not lower doses or vehicle, significantly decreases mechanical and cold allodynia for up to 240 min without producing motor deficits or sedation[2]. At doses of 10 to 40 mg/kg, Sinomenine hydrochloride dose-dependently increases the paw withdrawal threshold. In non-chronic constriction injury (CCI) healthy rats, Sinomenine hydrochloride at the dose range of 10 to 40 mg/kg does not change the immobility behavior in the forced swimming test[3].

    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (273.34 mM; Need ultrasonic and warming)

    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7334 mL 13.6668 mL 27.3336 mL
    5 mM 0.5467 mL 2.7334 mL 5.4667 mL
    10 mM 0.2733 mL 1.3667 mL 2.7334 mL
    *Please refer to the solubility information to select the appropriate solvent.
    Cell Assay

    The MDA-MB-231 human triple negative and 4T1 mouse breast cancer cell lines are used in this study. For the experiments, the cells are grown in 24-well plates at 3.5×104 cells/well. Following incubation for 24 or 48 h in medium containing different concentrations of Sinomenine hydrochloride, proliferation of the cells are detected with Cell Counting Kit-8 solution according to the manufacturer's instructions[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration

    Male Sprague-Dawley rats weighing of 250 to 300 g are used in this experiment. For the duration of action of acute Sinomenine hydrochloride study, different doses of Sinomenine hydrochloride (10 to 40 mg/kg) are administered 1 day after surgery and then paw withdrawal threshold is measured every 30 min for 4 hours. For the study involving daily Sinomenine hydrochloride treatment, mechanical hyperalgesia measure is performed 3 h after daily drug treatment. For antagonist studies, antagonists are given 10 min prior to 40 mg/kg Sinomenine hydrochloride administration[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight




    CAS No.



    OC(C1=C2C[[email protected]@]3([H])[[email protected]](C=C4OC)([H])[[email protected]@]1(CCN3C)CC4=O)=C(C=C2)OC.Cl


    Room temperature in continental US; may vary elsewhere

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    Product Name:
    Sinomenine hydrochloride
    Cat. No.:

    Sinomenine hydrochloride

    Cat. No.: HY-15122A