Fluorescent Dye
Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.
Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.
Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.
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Fluorescent Dye Related Products (4888)
Related Products (4888)
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Antibodies (4)
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5(6)-Aminofluorescein
0 ImagesSynonyms: 5(6)-AFM; Fluoresceinamine mixed isomers5(6)-Aminofluorescein (5(6)-AFM) is an amino-containing fluorescein dye primarily used for biomolecular labeling, fluorescence imaging, and as a precursor for the synthesis of fluorescent probes (Ex/Em = 495/515 nm).
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Ricinus Communis Agglutinin I (Biotinylated)
0 ImagesCat. No.: HY-NP0186Synonyms: RCA I (Biotinylated)Ricinus Communis Agglutinin I (Biotinylated) (RCA I (Biotinylated)) is a lectin and histochemical stain that binds to glycosylated residues of laminin A, B, and M chains. Ricinus Communis Agglutinin I (Biotinylated) acts as a sensitive probe for the localization of laminin heavy chains, and can also be used to purify laminin from skeletal muscle via lectin affinity chromatography. Ricinus Communis Agglutinin I (Biotinylated) is applicable to studies of intracerebrally transplanted tumors.
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Cy7 maleimide
0 ImagesCat. No.: HY-D2498Cy7 maleimide is a fluorescent dye containing a Maleimide (HY-W007324) functional group. Maleimide can be used to label thiol groups on antibodies, proteins and peptides.
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2-Hydroxy nile red trifluoromethanesulfonate
0 Images2-Hydroxy nile red trifluoromethanesulfonate is a fluorescent probe, that enters into oligodeoxyribonucleic acid as a nucleotide replacement. 2-Hydroxy nile red trifluoromethanesulfonate can be used as a DNA probe for detecting the polar changes in tumor microenvironments.
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6-FITC
0 Images6-FITC (6-Fluorescein Isothiocyanate) is a fluorescein isomer and also serves as a fluorescent label for biomolecules (FITC isomers have similar excitation/emission wavelengths, which are 495 nm/519 nm in fluorescence detection).
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Indocyanine green (Standard)
0 ImagesSynonyms: Foxgreen (Standard); IC Green (Standard); Cardiogreen (Standard)Indocyanine green (Standard) is the analytical standard of Indocyanine green. This product is intended for research and analytical applications. Indocyanine green (Foxgreen) is a low toxicic fluorescent agent that has been widely used in medical diagnostics, such as determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography.
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Cy5 HA (MW 500000)
0 ImagesCat. No.: HY-D2647Cy5 HA (MW 500000) is a hyaluronic acid labeled with CY5 (HY-D0821). Cy5 HA (MW 500000) is widely used for cell imaging and in vivo imaging of shallow tissue (Ex/Em = 633/670 nm).
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Leucocrystal violet
0 ImagesLeucocrystal violet is a cationic triarylmethane dye. Leucocrystal violet generates a colored purple product in the presence of hydrogen peroxide and horseradish peroxidase, or when oxidized by periodate under the catalysis of horseradish peroxidase. Leucocrystal violet serves as a chromogenic reagent for the determination of submicrogram-level hydrogen peroxide, with no interference from tryptophan, persulfate or chromate. Leucocrystal violet reacts with blood to produce a purple/violet color in the presence of hydrogen peroxide; it forms a very weak fluorophore when acting with whole blood in an unbuffered solution, with a maximum absorption wavelength of 630 nm and a maximum fluorescence emission wavelength of 665 nm upon excitation at 630 nm. Leucocrystal violet is widely used for staining blood residues on porous and non-porous materials.
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FITC-lysine-dextran (MW 10kDa)
0 ImagesCat. No.: HY-172164A
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LysoFP-NO2
0 ImagesLysoFP-NO2 is a turn-on fluorescent probe for carbon monoxide (CO) that localizes to the lysosome. In the presence of lysosomal CO, lysoFP-NO2 is transformed into lysoFP-NH2, which is highly fluorescent. LysoFP-NO2 is selective for CO over various reactive nitrogen, oxygen, and sulfur species. It displays excitation/emission maxima of 440/528 nm, respectively, and is not cytotoxic to HepG2 cells for up to five hours when used at a concentration of 30 μM.
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C12-NBD-ceramide
0 ImagesC12-NBD-ceramide is a fluorescent analogue of ceramide, it can be used as a substrate in ceramidase assays.
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TAMRA amine, 5-isomer hydrochloride
0 ImagesCat. No.: HY-W998707CAS No.: 2158336-48-0TAMRA amine, 5-isomer hydrochloride is a rhodamine dye with excitation/emission maximum 553/575 nm. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc.
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para-iodoHoechst 33258
0 Imagespara-iodoHoechst 33258 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
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FITC-Lithocholic acid 3-sulfate
0 ImagesCat. No.: HY-113238FSynonyms: FITC-Sulfolithocholic acidFITC-Lithocholic acid 3-sulfate (FITC-Sulfolithocholic acid) is a FITC-labeled Lithocholic acid 3-sulfate, which is a sulfated biliary metabolite. Lithocholic acid 3-sulfate selectively inhibits Th17 cell differentiation by targeting RORγt. Lithocholic acid 3-sulfate can be used for the research of pathogenesis of cholestatic liver diseases.
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CellTracker Orange CMRA Dye (5/6-mixture)
0 ImagesCellTracker Orange CMRA Dye is a fluorescent dye. CellTracker Orange CMRA Dye can be used for cell imaging and monitoring the movement and location of cells.
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Rhodamine B (Standard)
0 ImagesSynonyms: Basic Violet 10 (Standard); Brilliant Pink B (Standard); Rhodamine O (Standard); Tetraethylrhodamine (Standard)Rhodamine B (Standard) is the analytical standard of Rhodamine B. This product is intended for research and analytical applications. Rhodamine B is a staining fluorescent dye, commonly used for dyeing textiles, paper, soap, leather, and agents.
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Doxorubicin
0 ImagesCat. No.: HY-15142ACAS No.: 23214-92-8Synonyms: HydroxydaunorubicinDoxorubicin (Hydroxydaunorubicin), a cytotoxic anthracycline antibiotic, is an anti-cancer chemotherapy agent. Doxorubicin is a potent human DNA topoisomerase I and topoisomerase II inhibitor with IC50s of 0.8 μM and 2.67 μM, respectively. Doxorubicin reduces basal phosphorylation of AMPK and its downstream target acetyl-CoA carboxylase. Doxorubicin induces apoptosis and autophagy.
