pH Receptor Probe (solution) 

pH Receptor Probe (solution) is a pH-sensitive cyanine derivative and a environmental sensitive fluorophore. pH Receptor Probe has a pH-sensitive maximal absorption at 644 nm and emission at 664 nm. pH Receptor Probe has been used as a pH sensor of lysosomal compartments^[1].
Solvent and concentration: DMSO: 10 mM

pH Receptor Probe technology utilizes the general ability of GPCRs to be internalized into the endosomal pathway of a cell in response to agonist ligands. The pH Receptor Probe dye is fluorescent in acidic environments, but nonfluorescent at neutral pH. When pH Receptor Probe is bound to a receptor on the extracellular surface of the cell, it is essentially nonfluorescent. On internalization into a cell, it displays a significant increase in fluorescence^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

628.76

C₃₁H₃₆N₂O₈S₂

312961-83-4

Liquid

Dark blue to black

O=C(CCCCCN1C2=CC=C(S(=O)(O)=O)C=C2C(C)(C)/C1=C\C=C\C=C\C3=NC4=C(C3(C)C)C=C(S(=O)(O)=O)C=C4)O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Patrick MJ, et al. Intracellular pH measurements using perfluorocarbon nanoemulsions. J Am Chem Soc. 2013 Dec 11;135(49):18445-57.  [Content Brief]

  [2]. Elaine J Adie, et al. CypHer 5: a generic approach for measuring the activation and trafficking of G protein-coupled receptors in live cells. Assay Drug Dev Technol. 2003 Apr;1(2):251-9.  [Content Brief]