1. Neuronal Signaling
  2. Amyloid-β

β-Amyloid (1-42), rat TFA 

Cat. No.: HY-P1388A Purity: 95.17%
Handling Instructions

β-Amyloid (1-42), rat TFA is a 42-aa peptide, shows cytotoxic effect on acute hippocampal slices, and used in the research of Alzheimer's disease.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

β-Amyloid (1-42), rat TFA Chemical Structure

β-Amyloid (1-42), rat TFA Chemical Structure

Size Price Stock Quantity
500 μg USD 200 In-stock
Estimated Time of Arrival: December 31
1 mg USD 320 In-stock
Estimated Time of Arrival: December 31
5 mg USD 1200 In-stock
Estimated Time of Arrival: December 31
10 mg   Get quote  
50 mg   Get quote  

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Other Forms of β-Amyloid (1-42), rat TFA:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

β-Amyloid (1-42), rat TFA is a 42-aa peptide, shows cytotoxic effect on acute hippocampal slices, and used in the research of Alzheimer's disease.

IC50 & Target

Amyloid-β[1]

In Vitro

β-Amyloid (1-42), rat shows cytotoxic effect on the hippocampal slices at 20 μM[1]. β-Amyloid (1-42), rat causes morphological changes in NGF-induced PC12 cells, induces formed cell processes to retract in differentiated cells and affect the expression of exons 2/3 in both undifferentiated and differentiated cells[2].

Solvent & Solubility
In Vitro: 

10 mM in H2O

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.2207 mL 1.1033 mL 2.2065 mL
5 mM 0.0441 mL 0.2207 mL 0.4413 mL
10 mM 0.0221 mL 0.1103 mL 0.2207 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

After treating the hippocampal slices with 20 μM β-Amyloid (1-42) for 4 h, supernatant is replaced by fresh H-ASCF/3 (0.9 mL/chamber) to which 0.1 mL MTT stock solution (5 mg/mL H-ACSF/3) is added (MTT final concentration: 0.5 mg/mL). The chamber is left to rest for 15 min without carboxygenation. To stop further reduction of MTT, the medium (H-ACSF/3) is removed. The slices are transferred into 96-well plate, then pure DMSO (100 μL/slice/well) is added for dissolving formazane from the slices. (30 min in a 96-well plate). Then 70 μL DMSO solution from each slice (well) is transferred into another 96-well plate. The optical density (OD) of the dissolved formazane is measured at 550 and 620 nm[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

4532.04

Formula

C₁₉₉H₃₀₇N₅₃O₅₉S.C₂HF₃O₂

Storage
Powder -80°C 2 years
  -20°C 1 year
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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This equation is commonly abbreviated as: C1V1 = C2V2

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× = ×
C1   V1   C2   V2

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Product Name:
β-Amyloid (1-42), rat TFA
Cat. No.:
HY-P1388A
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β-Amyloid (1-42), rat TFA

Cat. No.: HY-P1388A