ALE-0540

Cat. No.: HY-124637 Pureté: 98.24%: Fiche technique
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ALE-0540, a nonpeptidic small molecule, is a nerve growth factor receptor antagonist. ALE-0540 inhibits the binding of NGF to tyrosine kinase (Trk) A or both p75 and TrkA with IC₅₀ values of 5.88 μM and 3.72 μM, respectively. ALE-0540 can be used to examine mechanisms leading to the development of agents for the study of pain.

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ALE-0540 Chemical Structure

CAS No. : 234779-34-1

Size	Stock	Quantité
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
50 mg	Obtenir un devis
100 mg	Obtenir un devis

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Recommandations en vedette

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Voir tous les produits spécifiques à Isoform Trk Receptor:

Voir toutes les isoformes

TrkA TrkB TrkC Trk

Activité biologique
Pureté et documentation
Références
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Description

IC₅₀ & Target

IC50: 5.88 μM (the binding of NGF to tyrosine kinase (Trk) A and TrkA); 3.72 μM (the binding of NGF to p75 and TrkA)^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
PC-12	EC₅₀	22 3 Compound: 1; ALE-0540	Inhibition of NGF-induced TrkA auto-phosphorylation in rat PC12 cells incubated for 15 mins by chemiluminescence based method Inhibition of NGF-induced TrkA auto-phosphorylation in rat PC12 cells incubated for 15 mins by chemiluminescence based method	[PMID: 27779399]
PC-12	EC₅₀	22 3 Compound: 1; ALE-0540	Inhibition of NGF-induced TrkA auto-phosphorylation in rat PC12 cells incubated for 15 mins by chemiluminescence based method Inhibition of NGF-induced TrkA auto-phosphorylation in rat PC12 cells incubated for 15 mins by chemiluminescence based method	[PMID: 27779399]
PC-12	EC₅₀	22 3 Compound: 1; ALE-0540	Inhibition of NGF-induced TrkA auto-phosphorylation in rat PC12 cells incubated for 15 mins by chemiluminescence based method Inhibition of NGF-induced TrkA auto-phosphorylation in rat PC12 cells incubated for 15 mins by chemiluminescence based method	[PMID: 27779399]
PC-12	IC₅₀	3.72 3 Compound: 1	Competitive binding against Nerve growth factor to p75-NGF receptor in PC12 cells Competitive binding against Nerve growth factor to p75-NGF receptor in PC12 cells	[PMID: 11931608]
PC-12	IC₅₀	3.72 3 Compound: 1	Competitive binding against Nerve growth factor to p75-NGF receptor in PC12 cells Competitive binding against Nerve growth factor to p75-NGF receptor in PC12 cells	[PMID: 11931608]
PC-12	IC₅₀	5.88 3 Compound: 1; ALE-0540	Inhibition of binding of [125I]-NGF to TrkA in rat PC12 cells incubated for 2 hrs by gamma counting method Inhibition of binding of [125I]-NGF to TrkA in rat PC12 cells incubated for 2 hrs by gamma counting method	[PMID: 27779399]
PC-12	IC₅₀	3.72 3 Compound: 1	Inhibition of NGF-stimulated Trk A in PC12 cells Inhibition of NGF-stimulated Trk A in PC12 cells	[PMID: 11931608]
PC-12	IC₅₀	5.88 3 Compound: 1; ALE-0540	Inhibition of binding of [125I]-NGF to TrkA in rat PC12 cells incubated for 2 hrs by gamma counting method Inhibition of binding of [125I]-NGF to TrkA in rat PC12 cells incubated for 2 hrs by gamma counting method	[PMID: 27779399]
PC-12	IC₅₀	5.88 3 Compound: 1; ALE-0540	Inhibition of binding of [125I]-NGF to TrkA in rat PC12 cells incubated for 2 hrs by gamma counting method Inhibition of binding of [125I]-NGF to TrkA in rat PC12 cells incubated for 2 hrs by gamma counting method	[PMID: 27779399]

Masse moléculaire

301.25

Formule

C₁₄H₁₁N₃O₅

CAS No.

234779-34-1

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1N(NCCO)C(C2=CC([N+]([O-])=O)=CC3=CC=CC1=C23)=O

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvant et solubilité

In Vitro:

DMSO : 125 mg/mL (414.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3195 mL	16.5975 mL	33.1950 mL
5 mM	0.6639 mL	3.3195 mL	6.6390 mL
10 mM	0.3320 mL	1.6598 mL	3.3195 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Working solution concentration: mg/mL

Pureté et documentation

Purity: 98.24%

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Fiche technique (274 KB) SDS (251 KB)

COA (252 KB) HNMR (272 KB) LCMS (96 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Owolabi JB, et al. Characterization of antiallodynic actions of ALE-0540, a novel nerve growth factor receptor antagonist, in the rat. J Pharmacol Exp Ther. 1999 Jun;289(3):1271-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.3195 mL	16.5975 mL	33.1950 mL	82.9876 mL
	5 mM	0.6639 mL	3.3195 mL	6.6390 mL	16.5975 mL
	10 mM	0.3320 mL	1.6598 mL	3.3195 mL	8.2988 mL
	15 mM	0.2213 mL	1.1065 mL	2.2130 mL	5.5325 mL
	20 mM	0.1660 mL	0.8299 mL	1.6598 mL	4.1494 mL
	25 mM	0.1328 mL	0.6639 mL	1.3278 mL	3.3195 mL
	30 mM	0.1107 mL	0.5533 mL	1.1065 mL	2.7663 mL
	40 mM	0.0830 mL	0.4149 mL	0.8299 mL	2.0747 mL
	50 mM	0.0664 mL	0.3320 mL	0.6639 mL	1.6598 mL
	60 mM	0.0553 mL	0.2766 mL	0.5533 mL	1.3831 mL
	80 mM	0.0415 mL	0.2075 mL	0.4149 mL	1.0373 mL
	100 mM	0.0332 mL	0.1660 mL	0.3320 mL	0.8299 mL

ALE-0540 Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ALE-0540234779-34-1ALE0540ALE 0540Trk ReceptorTropomyosin related kinase receptornonpeptidicnerve growth factor receptor antagonistp75TrkApainInhibitorinhibitorinhibit

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ALE-0540

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ALE-0540 Related Antibodies

Recommandations en vedette

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Voir tous les produits spécifiques à Isoform Trk Receptor:

Activité biologique

Pureté et documentation

Références

Avis client

Calculateur de molarité

Calculateur de dilution

Complete Stock Solution Preparation Table

ALE-0540 Related Classifications

Keywords:

Vos produits récemment consultés:

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