Carbonic anhydrase, Bovine erythrocytes  (Synonyms: EC 4.2.1.1)

Carbonic anhydrase, Bovine erythrocytes (EC 4.2.1.1) is ubiquitous zinc-containing metalloenzyme present in prokaryotes and eukaryotes. Carbonic anhydrase can catalyze reversible conversion of carbon dioxide to bicarbonate and protons. Carbonic anhydrase can be used for the research of cancer, glaucoma, obesity and epilepsy^[1][2].

Product Information
Molecular Weight:29.0 kDa (Theoretical)
Optimal pH: 7.0-7.5
Isoelectric Point: 6.40 (Theoretical)
Inhibitors:Monovalent anions, Sulfonates, sulfonamides, Imidazole

Instructions
Dissolve lyophilized powder in ice-cold water at a concentration of 0.1 mg/mL and store in an ice bath until use.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9001-03-0

Solid

White to off-white

[Carbonic anhydrase, Bovine erythrocytes]

4.2.1.1

≥3000 U/mg soild

The Unit is determined by the electrometric method of Wilbur and Anderson in which the time required (in seconds) for a saturated CO2 solution to lower the pH of 0.02 M Tris-HCl buffer from 8.3 to 6.3 at 0°C is determined. The time without enzyme is recorded as T0; with enzyme, T. A unit of activity = 2(T0 - T) divided by T.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Kanchan Aggarwal, et al. In Situ Photoregulation of Carbonic Anhydrase Activity Using Azobenzenesulfonamides. Biochemistry. 2019 Jan 8;58(1):48-53.  [Content Brief]

  [2]. Vladimir Amirkhanov, et al. Pharmacophores Modeling in Terms of Prediction of Theoretical Physicochemical Properties and Verification by EXPERIMENTAL correlations of Carbacylamidophosphates (CAPh) and Sulfanylamidophosphates (SAPh) Tested as New Carbonic Anhydrase Inhibitors. Mini Rev Med Chem. 2019;19(12):1015-1027.  [Content Brief]